Isolation of novel sequences targeting highly variable viral protein hemagglutinin

Abstract

Graphical abstract

Microbial Cells Harboring a Mitochondrial Gene Are Capable of CO2 Capture

“…PCR product was ethanol precipitated and dried. Double digestion with Xba I and BamH I was subsequently conducted for 5 h. pYES2/CT-ADH2 Vector 13 was triple digested with Bam H I, Xho I and Xba I 14 , and then ligated with double digested PCR product at 6 °C overnight. Ligation mixture was electroporated to E. coli ORIGAMI cells (Novagen, Madison, WI, USA) at 12.5 kV/cm, followed by plating to nitrogen fixation media (M9 media with NH 4 Cl removed, and supplemented with 1% sucrose as carbon source) 15 .…”

“…Mass Spectrometer: CO 2 reference gas was calibrated via USGS24 (δ 13 C PDB = −16‰) through two point corrections, and the results were corrected by USGS24 and IAEA600 (δ 13 C PDB = −27.5‰). N 2 reference gas was calibrated via IAEA N1 (δ 15 N air = 0.4‰), and the results were corrected by IAEA N1 and USGS43 (δ 14 N air = 8.44‰).…”