Isolation of polycyclic aromatic hydrocarbons (PAHs)-degrading Mycobacterium spp. and the degradation in soil

Zeng, Jun; Lin, Xiangui; Zhang, Jing; Li, Xuanzhen

doi:10.1016/j.jhazmat.2010.07.085

Cited by 98 publications

(40 citation statements)

References 45 publications

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“…Although both PHE and ANT have three rings, ANT has higher Log Kow (4.68) in the cultivation medium than PHE (4.50), which resulted in the lower degradation efficiency; while the lowest degradation efficiency of FLT among the three PAHs is attributed to its four aromatic rings and highest Log Kow (5.16). Similar results were observed in other studies [35][36][37][38][39].…”

Section: Discussionsupporting

confidence: 92%

“…The degradation rates of PAHs obtained in the present study were comparable with previous reports [37], but considerably higher than those reported by Zeng et al [39], and lower than those in the systems supplemented with pure cultivated bacteria and isolated microbial consortia [36,38]. The soil environment is complex and the difference in degradation rates may be attributed to differences in the soil properties, experimental conditions, and the concentration and composition of contaminants in the tested system.…”

Section: Discussionsupporting

confidence: 84%

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Bacteria capable of degrading anthracene, phenanthrene, and fluoranthene as revealed by DNA based stable-isotope probing in a forest soil

Song

Jiang

Zhang

et al. 2016

Journal of Hazardous Materials

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h i g h l i g h t s• Investigate PAHs degraders in forest carbon-rich soils via DNA-SIP.• Rhodanobacter is identified to metabolite anthracene for the first time.• The first fluoranthene degrader belongs to Acidobacteria.• Different functions of PAHs degraders in forest soils from contaminated soils. a r t i c l e i n f o a b s t r a c tInformation on microorganisms possessing the ability to metabolize different polycyclic aromatic hydrocarbons (PAHs) in complex environments helps in understanding PAHs behavior in natural environment and developing bioremediation strategies. In the present study, stable-isotope probing (SIP) was applied to investigate degraders of PAHs in a forest soil with the addition of individually 13 C-labeled phenanthrene, anthracene, and fluoranthene. Three distinct phylotypes were identified as the active phenanthrene-, anthracene-and fluoranthene-degrading bacteria. The putative phenanthrene degraders were classified as belonging to the genus Sphingomona. For anthracene, bacteria of the genus Rhodanobacter were the putative degraders, and in the microcosm amended with fluoranthene, the putative degraders were identified as belonging to the phylum Acidobacteria. Our results from DNA-SIP are the first to directly link Rhodanobacter-and Acidobacteria-related bacteria with anthracene and fluoranthene degradation, respectively. The results also illustrate the specificity and diversity of three-and four-ring PAHs degraders in forest soil, contributes to our understanding on natural PAHs biodegradation processes, and also proves the feasibility and practicality of DNA-based SIP for linking functions with identity especially uncultured microorganisms in complex microbial biota.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 84%

Bacteria capable of degrading anthracene, phenanthrene, and fluoranthene as revealed by DNA based stable-isotope probing in a forest soil

Song

Jiang

Zhang

et al. 2016

Journal of Hazardous Materials

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“…Similar to other low molecular weight (LMW) PAHs such as naphthalene and phenanthrene, anthracene can be degraded by a wide variety of microorganisms. Isolated bacteria capable of anthracene metabolism include organisms from both Gram-positive (Dean-Ross et al, 2001;Khan et al, 2002;Zeinali et al, 2007;Zhang et al, 2009;Zeng et al, 2010;Ling et al, 2011) and Gram-negative genera (Story et al, 2004;Jacques et al, 2005;Baboshin et al, 2008;Arulazhagan and Vasudevan, 2011;Jin et al, 2012), and several fungi have been described that are capable of transforming anthracene (Wu et al, 2010;Acevedo et al, 2011). However, only recently have culture-independent methods been applied to directly link uncultivated or uncharacterized bacteria to the metabolism of anthracene in contaminated environments.…”

Section: Introductionmentioning

confidence: 99%

Biostimulation Reveals Functional Redundancy of Anthracene-Degrading Bacteria in Polycyclic Aromatic Hydrocarbon-Contaminated Soil

Singleton²,

Aitken³

2013

Environmental Engineering Science

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Stable-isotope probing was previously used to identify bacterial anthracene-degraders in untreated soil from a former manufactured gas plant site. However, subsequent pyrosequence analyses of total bacterial communities and quantification of 16S rRNA genes indicated that relative abundances of the predominant anthracene-degrading bacteria (designated Anthracene Group 1) diminished as a result of biological treatment conditions in lab-scale, aerobic bioreactors. This study identified Alphaproteobacterial anthracene-degrading bacteria in bioreactor-treated soil which were dissimilar to those previously identified. The largest group of sequences was from the Alterythrobacter genus while other groups of sequences were associated with bacteria within the order Rhizobiales and the genus Bradyrhizobium. Conditions in the bioreactor enriched for organisms capable of degrading anthracene which were not the same as those identified as dominant degraders in the untreated soil. Further, these data suggest that identification of polycyclic aromatic hydrocarbon-degrading bacteria in contaminated but untreated soil may be a poor indicator of the most active degraders during biological treatment.

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“…So far, bacteria isolated from contaminated sites had been shown to be capable of degrading a range of PAHs (Cerniglia, 1992;Ye et al, 2014;Zeng et al, 2010). In our study, a high efficient pyrene-degrading bacterium PW belonging to genus Ochrobactrum was isolated after enrichment.…”

Section: Effect Of Bioaugmentation On Mineralization Of Pyrenementioning

confidence: 81%

Mineralization of pyrene induced by interaction between Ochrobactrum sp. PW and ryegrass in spiked soil

Liu

Wei

Qiao

et al. 2016

Ecotoxicology and Environmental Safety

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a b s t r a c tThis study was conducted to investigate the capability of pyrene-degrading bacterium Ochrobactrum sp. PW and ryegrass (Lolium multiflorum) grown alone and in combination on the degradation of pyrene in soil. After 60 days of ryegrass growth, plant biomass, pyrene-degrading microbial mass, soil enzyme activity (catalase activity and polyphenol oxidase activity) and residual concentration of pyrene in soils were determined. Higher dissipation rates were observed in PW inoculation treatments: ryegrassþ PW rhizosphere soil (RP-r) and ryegrassþ PW non-rhizosphere soil (RP-nr), than planting of ryegrass alone, rhizosphere (R-r) or non-rhizosphere (R-nr). The inoculation with PW significantly (p o0.05) increased the dry weight of ryegrass root and shoot, nearly 2.8 and 3.3 times higher than ryegrass treatment. The pyrene-degrading microbial mass indicated that a much larger mass of bacteria, actinobacteria were present in RP treatment. The catalase activity in all different treatments were significantly (p o 0.05) higher than in with treatment R-nr, and the polyphenol oxidase activity was also significantly (p o 0.05) increased by inoculation with PW, leading to enhanced mineralization of pyrene from soil. Our results suggest that adding of PAHs-degrading bacteria to soil can enhance remediation of PAHs contaminated soil, while improving plant growth.

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Isolation of polycyclic aromatic hydrocarbons (PAHs)-degrading Mycobacterium spp. and the degradation in soil

Cited by 98 publications

References 45 publications

Bacteria capable of degrading anthracene, phenanthrene, and fluoranthene as revealed by DNA based stable-isotope probing in a forest soil

Bacteria capable of degrading anthracene, phenanthrene, and fluoranthene as revealed by DNA based stable-isotope probing in a forest soil

Biostimulation Reveals Functional Redundancy of Anthracene-Degrading Bacteria in Polycyclic Aromatic Hydrocarbon-Contaminated Soil

Mineralization of pyrene induced by interaction between Ochrobactrum sp. PW and ryegrass in spiked soil

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