Isolation of porcine adenovirus from the respiratory tract of pigs in Japan.

Hirahara, Tadashi; Yasuhara, Hisao; Matsui, Osamu; Yamanaka, Morimasa; Tanaka, Masayuki; Fukuyama, Shin-ichi; Izumida, Akihiro; Yoshiki, Kenichi; Kodama, Kazuo; Nakai, M.; Sasaki, Norimasa

doi:10.1292/jvms1939.52.407

Cited by 27 publications

(19 citation statements)

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“…AF289262). PAdV5 was isolated for the first time in Japan from the respiratory tract of pigs, and the two reference strains (HNF-61 and HNF-70) were isolated from the same herd (19). To the best of our knowledge, this is the first time that PAdV5 has been identified in environmental samples and also as a common and probably subclinical infection of pigs in our area.…”

Section: Discussionmentioning

confidence: 70%

Identification of Human and Animal Adenoviruses and Polyomaviruses for Determination of Sources of Fecal Contamination in the Environment

Hundesa

Motes

Bofill-Mas

et al. 2006

Appl Environ Microbiol

150

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The Adenoviridae and Polyomaviridae families comprise a wide diversity of viruses which may be excreted for long periods in feces or urine. In this study, a preliminary analysis of the prevalence in the environment and the potential usefulness as source-tracking tools of human and animal adenoviruses and polyomaviruses has been developed. Molecular assays based on PCR specifically targeting human adenoviruses (HAdV), porcine adenoviruses (PAdV), bovine adenoviruses (BAdV), and bovine polyomaviruses (BPyV) were applied to environmental samples including urban sewage, slaughterhouse, and river water samples. PAdV and BPyV were detected in a very high percentage of samples potentially affected by either porcine or bovine fecal contamination, respectively. However, BAdV were detected in only one sample, showing a lower prevalence than BPyV in the wastewater samples analyzed. The 22 slaughterhouse samples with fecal contamination of animal origin showed negative results for the presence of HAdV. The river water samples analyzed were positive for the presence of both human and animal adenoviruses and polyomaviruses, indicating the existence of diverse sources of contamination. The identities of the viruses detected were confirmed by analyses of the amplified sequences. All BPyV isolates showed a 97% similarity in nucleotide sequences. This is the first time that PAdV5, BAdV6, and BPyV have been reported to occur in environmental samples. Human and porcine adenoviruses and human and bovine polyomaviruses are proposed as tools for evaluating the presence of viral contamination and for tracking the origin of fecal/urine contamination in environmental samples.

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Section: Discussionmentioning

confidence: 70%

Identification of Human and Animal Adenoviruses and Polyomaviruses for Determination of Sources of Fecal Contamination in the Environment

Hundesa

Motes

Bofill-Mas

et al. 2006

Appl Environ Microbiol

150

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“…Several strains have been isolated from pigs and are currently classified into five serotypes (Derbyshire et al, 1975 ;Hirahara et al, 1990). The proposed use of porcine adenoviruses (PAVs) as viral vaccine vectors (Tuboly et al, 1993) for the induction of mucosal immune responses against enteric viral antigens in pigs has generated great interest in understanding the molecular biology of these viruses.…”

Section: Introductionmentioning

confidence: 99%

“…The proposed use of porcine adenoviruses (PAVs) as viral vaccine vectors (Tuboly et al, 1993) for the induction of mucosal immune responses against enteric viral antigens in pigs has generated great interest in understanding the molecular biology of these viruses. PAV-3 was chosen as a candidate vector based on its low virulence and the ability to grow to high titres in cell culture (Derbyshire et al, 1975 ;Hirahara et al, 1990). The prototype strain (6618) of this virus was first isolated from a rectal swab collected from a healthy piglet (Clarke et al, 1967), and experimental infections of piglets with PAV-3 are subclinical or associated with transient diarrhoea (Derbyshire et al, 1975).…”

Section: Introductionmentioning

confidence: 99%

Development of porcine adenovirus-3 as an expression vector.

Reddy

Idamakanti

Hyun

et al. 1999

Journal of General Virology

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Porcine adenovirus-3 (PAV-3) was developed as an expression vector using homologous recombination in Escherichia coli BJ 5183. As a prerequisite, the complete genome of PAV-3 was first introduced as a PacI restriction fragment into a bacterial plasmid. The plasmid, when PacI restricted and transfected into swine testicular cells, produces an infectious virus. The potential of this procedure was demonstrated by the construction of several PAV-3 recombinants. Part of the E3 region, which is nonessential for virus replication under cell culture conditions, was identified and deleted from the virus genome. The gene for glycoprotein D (gD) of pseudorabies virus (PRV), which elicits PRV-neutralizing antibodies in pigs, was cloned and expressed from the E3 region of PAV-3. A 50 kDa polypeptide was identified in recombinant PAV-3-infected cell lysates by immunoprecipitation assays using gD-specific monoclonal antibodies. In another experiment, a region between the right inverted terminal repeat and the promoter of the E4 region was used to clone and express the chloramphenicol acetyltransferase (CAT) gene under the control of SV40 immediate early promoter. CAT gene expression was observed irrespective of the orientation of the CAT gene. These results indicate that the helper-independent recombinant PAV-3 could be used as an expression vector and has potential as a recombinant vaccine vector in pigs.

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“…Cross-neu tralization studies indicate the existence of at least five serotypes of PAV [3,4], of which PAV type 3 (PAV-3) replicated to the highest titres in cell culture [5]. The prototype strain of this virus was isolated from a rectal swab collected from a healthy piglet [6], and experimental infections There are few reports on molecular studies with PAV.…”

Section: Introductionmentioning

confidence: 99%

Restriction Endonuclease Analysis and Molecular Cloning of Porcine Adenovirus Type 3

Reddy¹,

Nagy²,

Derbyshire³

1993

Intervirology

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The 6618 strain of porcine adenovirus type 3 was cultivated in swine testis cells, and viral DNA was extracted from the infected cells by a modified Hirt procedure. The DNA was digested by each of 13 restriction endonucleases, and the number of cleavage sites which were identified varied from 1 to 17. The size of the porcine adenovirus type 3 genome, estimated from the sizes of the restriction enzyme fragments, was approximately 35 kb. Fragments representing the entire genome were cloned. Physical maps were constructed based on the results of restriction endonuclease analyses and hybridization and oriented with respect to the genome of bovine adenovirus type 3.

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Isolation of porcine adenovirus from the respiratory tract of pigs in Japan.

Cited by 27 publications

References 0 publications

Identification of Human and Animal Adenoviruses and Polyomaviruses for Determination of Sources of Fecal Contamination in the Environment

Identification of Human and Animal Adenoviruses and Polyomaviruses for Determination of Sources of Fecal Contamination in the Environment

Development of porcine adenovirus-3 as an expression vector.

Restriction Endonuclease Analysis and Molecular Cloning of Porcine Adenovirus Type 3

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