Isolation of Rat Male Accessory Glands Autoantigens by Affinity Chromatography*

Pesoa, Susana; Pistoresi-Palencia, María C.; Galmarini, Mirian; Vottero-Cima, E; Riera, Clelia M.

doi:10.1111/j.1600-0897.1983.tb00255.x

Cited by 9 publications

(5 citation statements)

References 14 publications

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“…It should be noted, however, that the antisera used in most studies have been generated in a foreign species and that the components identified may not be antigens in terms of the host. Nevertheless, some putative autoantigens have been identified that are produced by the epididymis (Vernon et al, 1982) and the male accessory sex glands (Pesoa et al, 1983). Also, most studies have not ruled out the possibility of contributions by sperm to male excurrent duct fluids used for immunization or assays, but one study has demonstrated that epididymal fluid from immature rats and from seminal vesicles, which have not been in contact with sperm, contains components also detected on ejaculated sperm from mature rats (Dravland and Joshi, 1981).…”

Section: Antisera Have Been Used To Study the Role Of Epididymal Secrmentioning

confidence: 97%

Immunodissection of sperm surface modifications during epididymal maturation

et al. 1985

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Mammalian spermatozoa undergo changes in morphology, composition, and function during transit through the epididymis. These changes correlate with acquisition by sperm of the ability to fertilize ova. It has been found that sperm from the cauda epididymidis, but not those from the caput epididymidis, are able to bind to the zona pellucida. This would imply a modification in sperm surface characteristics. Biochemical and immunological studies have demonstrated changes in sperm surface composition during epididymal maturation. These changes involve addition of epididymal secretory products to the sperm surface, loss or alteration of existing sperm surface molecules, and possibly the unmasking of preexisting molecules or epitopes. Several laboratories have studied the epididymal secretory proteins in the rat, but a consensus has not been reached on the identification, characterization, source, and sperm surface association of these proteins. Monoclonal antibodies are beginning to be used to characterize sperm surface components and sperm maturation antigens. They are proving to be valuable tools for the dissection of epididymal maturation when used in conjunction with biochemical and physiological approaches.

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Section: Antisera Have Been Used To Study the Role Of Epididymal Secrmentioning

confidence: 97%

Immunodissection of sperm surface modifications during epididymal maturation

et al. 1985

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“…The present investi gations were initiated preparing from RAG a homo geneous preparation of the antigen under study [7] for both chemical and immunological research.…”

Section: Discussionmentioning

confidence: 99%

“…The purified antigen was obtained as described previously by immunoaffinity chromatography, using the autologous anti-RAG serum as ligand [7]. This material was chemically modified as de scribed for MRAG.…”

Section: Antigensmentioning

confidence: 99%

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Characterization of an Antigen from Rat Male Accessory Glands

Pesoa¹,

Galmarini²,

Pistoresi-Palencia³

et al. 1986

Int Arch Allergy Immunol

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An isolated soluble antigen involved in the autoimmune response against rat male accessory glands migrated as a double band when it was submitted to analytical disc polyacrylamide gel electrophoresis and as a homogeneous molecular species in SDS electrophoresis. The purified antigen had a molecular weight of approximately 78 K by SDS electrophoresis and 80 K by gel filtration chromatography. The antigen was identified as a protein and displays androgen dependency. The purified fraction was pathogenically active in microgram doses and induced humoral and delayed-type hypersensitivity responses. Besides, lesions were observed in the target organ. The cellular infiltration was located in prostate glands according to the localization of antigen by immunofluorescence findings.

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“…Chemical modification does not change the specificity but plays an adjuvant role (10). The autologous antigen involved in that response, as purified by affinity chromatography, appears late in the ontogeny and is not present in females (12). Further reports appeared describing the involvement of the immunoregulatory mechanisms in this phenomenon (13)(14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%