Isolation of Stigmasterol and β-Sitosterol from Chloroform Extract 
 of Leaves of Corchorus fascicularis Lam.

Rajput, Ambarsing P.; Rajput, T. A.

doi:10.3923/ijbc.2012.130.135

Cited by 32 publications

(27 citation statements)

References 3 publications

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“…The test for steroid was performed by Liebermann–Burchard reaction as described by Rajput and Rajput ( 2012 ). A few crystals of isolated solid compound were dissolved in chloroform, and a few drops of concentrated sulfuric acid were added to the solution followed by the addition of 3 drops of acetic anhydride.…”

Section: Methodsmentioning

confidence: 99%

Structure elucidation of β-sitosterol with antibacterial activity from the root bark of Malva parviflora

2016

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The powder of root bark of Malva parviflora (Malvaceae) was successively extracted with petroleum ether (b.p. 60-80 °C), chloroform and ethanol. The chloroform extract showed antibacterial activity against Staphylococcus aureus and Escherichia coli, whereas the ethanolic extract showed antibacterial activity against only S. aureus. The chloroform extract, after column chromatographic separation on silica gel using petroleum ether:chloroform (3:1) as eluent, furnished 98 mg of white crystalline compound. The yield of the compound is 0.316 % (w/w). The compound has a melting point of 134-136 °C and the R f value 0.56 in benzene:chloroform:acetone (1:15:1) on silica gel TLC. The compound was characterized as β-sitosterol by physical properties, chemical test, spectral analysis (FTIR, NMR and MS) and comparing the data obtained from the literature.

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Section: Methodsmentioning

confidence: 99%

Structure elucidation of β-sitosterol with antibacterial activity from the root bark of Malva parviflora

2016

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“…Similarly one three proton doublet at 0.83 for H-26 and other three proton doublet at 0.85 assigned to H-27, both attached to C-25. In the 13 Comparison of its physical and spectral data with published values [14,15] confirmed the identity of the compond 3 as Betasitosterol. Table 1 summarizes the results obtained for antimicrobial activity of the Abutilon indicum plant leaves extract.…”

Section: Compoundmentioning

confidence: 69%

Phytochemical Analysis and Antimicrobial Activity of Chloroform Extract of <i>Abutilon indicum</i>

Singh¹

2016

AJAC

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Powdered Abutilon indicum leaves were extracted from chloroform. Thin layer chromatography technique in combination with column chromatography was used to separate the chemically active constituents. The constituents were subjected to IR, NMR, EIMS analysis to identify the compounds contained in the extract. The antimicrobial activity of the plant extract was examined on Bacillus subtils, Klebsiella pneumonia, Pseudomonas aeruginosa, E.coli and the effect was compared with that of chloramphenicol. The comparative results showed that chloroform extract of Abution indicum can serve well as an antimicrobial agent.

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“…The results of the spectroscopic characterization and antimicrobial activities of stigmasterol and βsitosterol from Mangifera indica root are reported in Tables 1 and 2 The DEPT 135 NMR spectrum showed three quaternary carbons, eleven methine, nine methylene, and six methyl carbons [9]. The The 1D NMR, 2D NMR, 1R and melting point and their comparison [11] identified NPJ 68 to be a mixture of stigmasterol and -sitosterol.…”

Section: Resultsmentioning

confidence: 99%

Stigmasterol and β-sitosterol from the Root of Mangifera indica and Their Biological Activities against Some Pathogens

Nna

Tor-Anyiin

Muluh

2019

JOCAMR

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Aim: Mangifera indica is a plant with several phytoconstituents which accounts for its trado-pharmacological uses such as treatment of syphilis, anemia, diabetes, diarrhea, mouth wound, ulcer, leucorrhea, fever, liver diseases, urinary tract infection, asthma, rheumatism, cough, colon cancer and dysentery. The present study was aimed at identifying and characterizing some of the active principles from root of the plant. Places and Duration of Study: The isolation and characterization of the compounds was carried out at the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, Glasgow, United Kingdom between October, 2018 to February, 2019 while the bioassay analysis was done at Ahmadu Bello University, Zaria, Nigeria. Methodology: The root powder was subjected to Soxhlet extraction with ethyl acetate to obtain the crude extract, which was fractionated on column using hexane, and ethyl acetate in increasing ratios. The isolated components were tested for their antimicrobial activities against some plants and animal pathogens at Ahmadu Bello University, Zaria, Nigeria. Results: White crystals were obtained which on spectra analysis (IR, 1H-NMR, 13C-NMR, 2D-NMR) were identified as mixture of stigmasterol and β-sitosterol. The isolated compounds exhibited appreciable antimicrobial activities against some microbes, thus confirming the many ethnomedical uses of the plant. Conclusion: The compounds isolated were identified as stigmasterol and β-sitosterol. The pathogens showed moderate sensitivity towards the isolated compounds in the study.

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Isolation of Stigmasterol and β-Sitosterol from Chloroform Extract of Leaves of Corchorus fascicularis Lam.

Cited by 32 publications

References 3 publications

Structure elucidation of β-sitosterol with antibacterial activity from the root bark of Malva parviflora

Structure elucidation of β-sitosterol with antibacterial activity from the root bark of Malva parviflora

Phytochemical Analysis and Antimicrobial Activity of Chloroform Extract of <i>Abutilon indicum</i>

Stigmasterol and β-sitosterol from the Root of Mangifera indica and Their Biological Activities against Some Pathogens

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Isolation of Stigmasterol and β-Sitosterol from Chloroform Extract of Leaves of Corchorus fascicularis Lam.

Cited by 32 publications

References 3 publications

Structure elucidation of β-sitosterol with antibacterial activity from the root bark of Malva parviflora

Structure elucidation of β-sitosterol with antibacterial activity from the root bark of Malva parviflora

Phytochemical Analysis and Antimicrobial Activity of Chloroform Extract of &lt;i&gt;Abutilon indicum&lt;/i&gt;

Stigmasterol and β-sitosterol from the Root of Mangifera indica and Their Biological Activities against Some Pathogens

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Phytochemical Analysis and Antimicrobial Activity of Chloroform Extract of <i>Abutilon indicum</i>