Isolation of Thermophilic Lignin Degrading Bacteria from Oil-Palm Empty Fruit Bunch (EFB) Compost

Lai, Chenhuan; Chua, Han Bing; Danquah, Michael K.; Saptoro, Agus

doi:10.1088/1757-899x/206/1/012016

Cited by 22 publications

(11 citation statements)

References 22 publications

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“…Gram-positive bacterial isolates were higher than gram-negative bacterial isolates. These results are in line with Lai et al (2017) who also reported that the results of isolation of oil palm empty fruit bunches compost were dominated by gram-positive bacteria (8 bacterial isolates) followed by gram-negative bacteria (2 bacterial isolates). According to Mistou et al (2016), gram-positive bacteria have cell walls that contain thick peptidoglycan while gram-negative bacteria have thin peptidoglycan content.…”

Section: Resultssupporting

confidence: 91%

“…Also, Harith et al (2014) reported that 24 bacterial isolates were recovered from oil palm industry waste. Besides, Lai et al (2017) obtained 34 bacterial isolates that were successfully isolated from compost oil palm empty fruit bunches. The abundance of bacteria can also be obtained through extract suspension of oil palm empty fruit bunches in the form of a local microorganism solution.…”

Section: Introductionmentioning

confidence: 99%

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Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions

et al. 2020

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Abstract. Dermiyati, Suharjo R, Telaumbanua M, Yosita R, Sari AW, Andayani AP. 2020. Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions. Biodiversitas 21: 4213-4220. Increased production of palm oil causes an increase in oil palm empty fruit bunches waste, while the utilization of the waste has not been carried out optimally. Oil palm empty fruit bunches contain high cellulose content which promotes the development of various types of bacteria with different characteristics. This study was aimed to determine the abundance and the characterization of bacteria that were isolated from extract suspension of oil palm empty fruit bunches in the form of a local microorganism solution under aerobic, anaerobic, and facultative anaerobic conditions. For isolation, the oil palm empty fruit extract suspension was inoculated on media Plate Count Agar Peptone and bacterial abundance was determined. Various bacterial characterization test included bacterial morphology test in the form of color and shape of macroscopic isolates, gram test using KOH 3%, fermentative oxidative test, soft rot test on potato tubers, hypersensitivity test on tobacco plant leaves, and hypo virulent test which was indicated by the presence of symptoms of disease in cucumber sprouts and indicated in the disease severity index (DSI) was carried out. The results of local microorganism solution isolation from oil palm empty fruit bunches obtained as many as 220 bacterial isolates consisting of 84 isolates in aerobic condition, 68 isolates in anaerobic condition, and 68 isolates in facultative anaerobic condition. The highest bacterial abundance in aerobic conditions was equal to 285.60 x 1010 CFU mL-1. Each bacterial isolate had different characteristics. Most of the characteristics of bacteria were turbid white (34.55%), round (69.55%), gram-positive (76.36%), fermentative (89.55%), softrot negative (71.82%), hypersensitive negative (97.27%), and virulent (74.55%).

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions

et al. 2020

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“…These strains showed very low MnP activity (data not shown), while LiP activity was not observed (Chang et al 2014). In contrast to the results obtained in present study, Lai and co-worker worked on thermophilic lignin-degrading bacteria and reported that out of ten isolates CLMT 18 (0.7475 U/ml) and CLMT 34 (0.8178 U/ml) showed the maximum laccase activity, while CLMT5 (1.8759 U/ml), CLMT 18 (1.6684 U/ ml) and CLMT 20 (1.5322 U/ml) showed maximum MnP activity (Lai et al 2017). …”

Section: Periodic Monitoring Of Modified Media Degradation By Axenic supporting

confidence: 64%

Extraction of ligninolytic enzymes from novel Klebsiella pneumoniae strains and its application in wastewater treatment

2018

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The presence of lignin and its derivatives in pulp effluent increases the pollution load on the environment. With an aim of better degradation and decolourization of wastewater, this study proposes the utilization of different ligninolytic enzymes from novel bacterial strains. Four novel bacterial strains of Klebsiella pneumoniae (K. pneumoniae strains NITW715076, NITW715076_1, NITW715076_2 and NITW715076_3) were isolated and identified. The ligninolytic enzymes were characterized by plate assay method. For the optimization of various process parameters of effluent sample, different approaches were used like one factor at a time and statistical optimization through response surface methodology (RSM). Further to validate the above data, enzyme activity, total phenolic concentration, GC-MS analysis and seed germination test were also performed. The ligninolytic enzymes produced were characterized as laccase and Manganese peroxidase (MnP). In lignin degradation and decolourization studies, consortia 1 (K. pneumoniae NITW715076_2 + K. pneumoniae NITW715076_1) (82.31%) was found more effective when compared to axenic culture (K. pneumoniae NITW715076_2) (74.1%). In RSM studies, Laccase and MnP activities were increased by 20% and 18%, respectively, as compared to one factor at a time optimization method. In addition, the enzyme activity for laccase and MnP after prediction by RSM was found 53338 IU/L and 147900 IU/L, respectively. The R 2 values for both the enzymes were found to be significant. Further, GC-MS analysis also showed the degradation of different organic pollutants in effluent. Lastly, the seed germination test using consortia 1 corroborated the evidence of detoxification of industrial effluent. Effluent treated by consortia 1 showed better results in degradation and decolourization of lignin and their derivatives. Therefore, consortia 1 can be used for various industrial purposes like fruit juice clarification, diagnostic purposes and enhanced bioethanol production.

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“…Based on the preliminary screening result, 10 microbial isolates were identified to possess at least one ligninolytic enzyme activity, which may be potential for lignin degradation. Interestingly, three microbial isolates, CH2, CH5 and CH9 showed the ability to decolourise all four synthetic dyes, thus suggesting the isolates may also produce laccase, LiP, and MnP, which are crucial to degrade lignin into simpler substrates (Lai et al, 2017). Three isolates exhibited a dominant decolourisation zone compared to the others, insinuating that the isolates have a high potential to degrade lignin better or faster than other tested microbes in this study.…”

Section: Resultsmentioning

confidence: 62%

Qualitative and Molecular Screening of Potential Ligninolytic Microbes from Termite (Coptotermes curvignathus) Gut

Simol¹,

Chubo²,

King³

et al. 2021

BJRST

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Ligninolytic microbes have great potential in converting high lignin by-products to more utilisable products by decomposing the lignin-rich agricultural and industrial wastes. Thus, the aim of this study are to screen and identify the potential ligninolytic microbes from the termite (Coptotermes curvignathus) gut. The study was conducted at Universiti Putra Malaysia Bintulu Sarawak Campus, Malaysia. Twenty-seven microbes isolated from termite gut obtained from the Microbiology Laboratory, Faculty of Agricultural Science and Forestry, were used for the ligninolytic activity screening. Media with four different ligninolytic indicator dyes (Azure B, phenol red, methylene blue, and Remazol Brilliant Blue) were streaked with microbial isolates and incubated at 37 °C for 48 h. Out of twenty-seven microbe isolates, only three (CH2, CH5, and CH9) isolates showed decolourisation zone indicating the positive presence of ligninolytic activity. The 16S rRNA gene sequence data indicated the isolates are highly homologous to Bacillus spp.

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Isolation of Thermophilic Lignin Degrading Bacteria from Oil-Palm Empty Fruit Bunch (EFB) Compost

Cited by 22 publications

References 22 publications

Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions

Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions

Extraction of ligninolytic enzymes from novel Klebsiella pneumoniae strains and its application in wastewater treatment

Qualitative and Molecular Screening of Potential Ligninolytic Microbes from Termite (Coptotermes curvignathus) Gut

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