Tanah pasca galian C merupakan tanah yang bermasalah karena rendahnya unsur hara dan C-organik. Penelitian ini bertujuan untuk mengetahui pengaruh inokulasi FMA dan penambahan bahan organik dalam meningkatkan pertumbuhan dan serapan hara P tanaman jagung pada tanah pasca penambangan galian C. Perlakuan disusun secara faktorial 3×4 dalam Rancangan Acak Kelompok (RAK) dengan 3 kelompok. Faktor pertama, inokulasi FMA dengan 3 taraf, yaitu: tanpa FMA (0 spora), 250 spora, dan 500 spora. Faktor kedua, penambahan bahan organik dengan 4 taraf, yaitu: tanpa bahan organik (0%), 20%, 40%,dan 60% dari volume tanah. Data diuji dengan analisis ragam kemudian dilanjutkan dengan uji nilai tengah menggunakan BNT pada á=5%. Hasil penelitian menunjukkan bahwa inokulasi FMA meningkatkan infeksi akar dan pertumbuhan tanaman jagung melalui volume akar. Hasil infeksi akar meningkat setelah diberikan FMA (250 spora dan 500 spora), sedangkan volume akar tertinggi adalah pada inokulasi dosis 250 spora. Penambahan bahan organik meningkatkan infeksi akar dan serapan haraP tanaman jagung. Hasil infeksi akar tertinggi pada perlakuan tanpa bahan organik (0%) dan dosis 60% bahan organik. Sedangkan hasil serapan hara P tertinggi ditunjukkan pada dosis 40% bahan organik. Interaksi FMA dan bahan organik terjadi pada tinggi tanaman 2 MST. Hasil tinggi tanaman terbaik ditunjukkan pada kombinasi FMA 250 spora dengan tanpa bahan organik yaitu 59,97 cm

Abstract. Dermiyati, Suharjo R, Telaumbanua M, Ilmiasari Y, Yosita R, Annisa RM, Sari AW, Andayani AP, Yulianti DM. 2019. Population of phosphate solubilizing bacteria in the liquid organic fertilizer created from palm oil bunches and pineapple rhizome. Biodiversitas 20: 3315-3321. Palm oil bunches and pineapple rhizomes are abundant in Indonesia, especially in Lampung Province due to widespread agro-industrial company and they are potential to be used as sources for liquid organic fertilizer. This study was aimed to reveal microbial population within liquid organic fertilizer (LF) which was developed from palm oil bunches (PB) and pineapple rhizome (PR) and their role as phosphate solubilizing bacteria (PSB). The capability to solubilize phosphate was recorded from the clear zone area around the bacterial colony which was cultivated on Pikovskaya’s agar medium. The LF was prepared in three kinds of conditions, namely aerobic, anaerobic, and facultative an aerobic. Isolation was performed every 3 days until 24 days after incubations. The results showed that microbial population in the LF from PR was significantly higher than those developed from PB. Totally, 791 bacterial isolates were obtained from the LF; 490 isolates were achieved from PR (153 isolates from aerobic, 188 isolates from facultative anaerobic, 149 from anaerobic conditions) and 301 isolates were collected from PB (96 isolates from aerobic, 112 isolates from facultative anaerobic, 93 isolates from anaerobic conditions). In the case of isolates that were gained from PB, 166 isolates (55.15%) showed capability to degrade phosphate and 135 isolates (44.85%) did not show any capability as PSB. As for isolates obtained from PR, 269 isolates (54.90%) had capability to solubilize phosphate, meanwhile, the other 221 isolates (45.10%) did not show any capability to solubilize phosphate. Among166 isolates of PSB from PB, 60 isolates had very low capability, 55 isolates had low capability, 34 isolates had medium capability, 10 isolates had high capability and only 7 isolates had very high capability. Meanwhile, from 269 isolates of PSB obtained from PR, 135 isolates had very low capability to solubilize phosphate, 84 isolates had low capability to solubilize phosphate, 32 had medium capability to solubilize phosphate, 14 isolates had high capability to solubilize phosphate and 4 isolates produced very high capability as PSB. In total, 11 chosen isolates were selected from LF developed from both PB (7 isolates) and PR (4 isolates). Therefore, palm oil bunches and pineapple rhizomes contained PSB that can be used as LF.

Limbah domestik (greywater) sebagian besar dibuang langsung dalam badan air tanpa adanya pengolahan karena biaya yang mahal, penerapan yang sulit dan teknologi yang belum terjangkau masyarakat, sehingga dapat mencemari lingkungan. Pengolahan air limbah domestik yang efisien, murah, mudah dan ramah lingkungan harus dikembangkan. Fitoremediasi merupakan salah satu pengolahan air limbah dengan menggunakan agen biologi gulma air. Penelitian ini bertujuan untuk mengetahui efektifitas fitoremediasi menggunakan kombinasi beberapa jenis gulma air Eceng gondok (Eichhornia crassipes (Mart)), Kiambang (Salvinia molesta) dan Kayu apu (Pistia Stratiotes L.) dalam memperbaiki kualitas air limbah domestik. Rancangan percobaan yang digunakan perlakuan faktorial 8 x 2 dalam Rancangan Acak Kelompok dengan 3 ulangan. Faktor pertama adalah jenis gulma dengan 8 level yaitu tunggal, kombinasi 2 gulma dan kombinasi 3 gulma Faktor kedua adalah waktu pengamatan dengan 2 level yaitu 4 hari dan 8 hari. Parameter yang diukur adalah pH, COD, BOD, TSS, amonia dan minyak lemak. Hasil penelitian menunjukkan bahwa gulma Eceng gondok, Kiambang, Kayu apu perlakuan tunggal, kombinasi 2 jenis gulma dan kombinasi 3 jenis gulma sangat efektif dan sama baiknya dalam menaikan pH dan menurunkan COD, BOD, TSS, amonia dan minyak lemak air limbah domestik pada hari ke-4 maupun hari ke-8 sampai memenuhi baku mutu yang disyaratkan oleh kementrian Lingkungan Hidup dan Kehutanan No. 68 Tahun 2016.

This research aimed to evaluate the residual Cu and Zn in tropical soil over 21 years after amendment with industrial waste, lime, and compost. Soil samples were collected from a well-maintained experimental field amended one time with industrial waste high in Cu and Zn set in 1998 at about 1.5–20 years after amendment. Treatments were arranged in a randomized block design with a metal-wares industrial waste at rates 0, 15, and 60 Mg·ha−1, lime at 0 and 5 Mg·ha−1, and compost at 0 and 5 Mg·ha−1. Soil samples were also taken vertically in the exact plot centers at depths of 0–75 cm of plots not amended with lime and compost and also from topsoils (0–15 cm) at 90 points with distances 50 cm between each other in each of the respective plots 21 years after amendment. Significant increases in Cu and Zn caused by waste were observed over 21 years. The increase in soil pH by lime was observed over 10 years but its effect in decreasing Cu and Zn was observed only at 1.5 years, particularly in topsoils. However, the effect of lime in lowering Cu and Zn was observed over 20 years when waste and/or compost were also given. Similarly, the effect of compost was absent over 20 years but significant when waste and/or lime were also given. The relative concentration of Cu and Zn in the 60 Mg waste ha−1 plots decreased over 20 years to 17–53% for Cu and to 12–33% for Zn; their concentrations were, in general, lower with lime and/or compost addition. The relative concentrations of Cu and Zn significantly decreased over 21 years in plots with 15 or 60 Mg·ha−1 and increased in the control plots. Heavy metals moved in soil significantly through soil tillage and erosion and partially by leaching.

The field and laboratory experimental studies were conducted to determine the phosphorus (P) adsorption behavior employing Langmuir Method and yield of sugarcane as affected by compost and inorganic fertilizer applications, and to determine the correlations between the Langmuir parameters and the availability of P in soil or harvested P by the sugarcane. Both field and lab experiments were arranged in a randomized block design, consisting of five treatments (A= 100% NPK, B=100% compost, C=100% NPK + 50% compost, D= 50% NPK + 100%, compost, and E= no fertilizer).  Each treatment was replicated three times. Field and laboratory studies were carried out in the sugarcane field and in the Laboratory of Soil Science, the University of Lampung for Langmuir P studies, respectively. The results showed that the applications of 100% NPK plus 50% organic fertilizer, and 100% NPK resulted in the highest amount of available-P in the soil, the lowest maximum adsorption of P (Xm) and the lowest relative bonding energy of P (km); as well as the highest effect on the increase of sugarcane production. On the other hand, the treatments of no fertilizer and the 100% organic fertilizer resulted in the highest maximum adsorption of P (Xm) and the lowest sugar production. There were very significant negative correlations between the maximum adsorption of P (Xm) and soil P, harvested P by the sugarcane and stem biomass; while similar trend but no significant correlations between the relative bonding energy of P (km) and soil P, harvested P, leaf biomass and stem biomass were detected.