Isolation of tissue layers in hermatypic corals by N-acetylcysteine: morphological and proteomic examinations

Peng, Shao-En; Luo, Yi-Jyun; Huang, Huai-Ting; Lee, I.-T.; Hou, Lei; Chen, W.-N. U.; Fang, Lee‐Shing; Chen, C.-S.

doi:10.1007/s00338-007-0300-4

Cited by 28 publications

(22 citation statements)

References 20 publications

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“…One group has made some progress along these lines by separating gastrodermal from ectodermal tissue using the reducing agent N-acetylcysteine (NAC) (Peng et al, 2008). The NAC treatment was able to separate tissue layers without inducing protein degradation, however, it is not clear if the RNA was intact following this treatment.…”

Section: Functional Categorymentioning

confidence: 98%

Coral larvae exhibit few measurable transcriptional changes during the onset of coral-dinoflagellate endosymbiosis

Schnitzler

Weis

2010

Marine Genomics

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Section: Functional Categorymentioning

confidence: 98%

Coral larvae exhibit few measurable transcriptional changes during the onset of coral-dinoflagellate endosymbiosis

Schnitzler

Weis

2010

Marine Genomics

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“…After rinsing with FSW, tentacle tips were removed using microscissors (Spring Type, AESCULAP Inc., Center Valley, PA, USA) to prevent interference from nematocytes. The gastroderm was then separated from the epiderm by incubation with 3% N-acetylcysteine (pH 8.2, prepared in ASW) for 1-2 h at RT (Peng et al 2008), and isolated gastroderms were homogenized on ice using a 7-ml glass tissue grinder (Kimble/Kontes, Vineland, NJ, USA) at 20-30 passes in 1 ml homogenization buffer (250 mM sucrose in ASW, pH 8.2). Under such homogenization, gastrodermal cells were broken to release their intracellular LBs, while Symbiodinium cells remained intact.…”

Section: Lb Isolation and Lipid Analysismentioning

confidence: 99%

Diel rhythmicity of lipid-body formation in a coral-Symbiodinium endosymbiosis

et al. 2011

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The biogenesis of intracellular lipid bodies (LBs) is dependent upon the symbiotic status between host corals and their intracellular dinoflagellates (genus Symbiodinium), though aside from this observation, little is known about LB behavior and function in this globally important endosymbiosis. The present research aimed to understand how LB formation and density are regulated in the gastrodermal tissue layer of the reef-building coral Euphyllia glabrescens. After tissue fixation and labeling with osmium tetroxide, LB distribution and density were quantified by imaging analysis of serial cryo-sections, and a diel rhythmicity was observed; the onset of solar irradiation at sunrise initiated an increase in LB density and size, which peaked at sunset. Both LB density and size then decreased to basal levels at night. On a seasonal timescale, LB density was found to be significantly positively correlated with seasonal irradiation, with highest densities found in the summer and lowest in the fall. In terms of LB lipid composition, only the concentration of wax esters, and not triglycerides or sterols, exhibited diel variability. This suggests that the metabolism and accumulation of lipids in LBs is at least partially light dependent. Ultrastructural examinations revealed that the LB wax ester concentration correlated with the number of electron-transparent inclusion bodies. Finally, there was a directional redistribution of the LB population across the gastroderm over the diel cycle. Collectively, these data reveal that coral gastrodermal LBs vary in composition and intracellular location over diel cycles, features which may shed light on their function within this coral-dinoflagellate mutualism.

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“…Alternately, coral soft tissue is chemically induced to detach, in response to removal of divalent cations (Gates and Muscatine 1992;Frank et al 1994;Kopecky and Ostrander 1999;Domart-Coulon et al 2001, 2004aMass et al 2012) or exposure to a reducing agent, such as N-acetylcysteine (Peng et al 2008). Enzymatic digestion has also been widely used to dissociate the tissue into single cells (Gates and Muscatine 1992;Frank et al 1994;Helman et al 2008;Downs et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Scleractinian coral cell proliferation is reduced in primary culture of suspended multicellular aggregates compared to polyps

et al. 2013

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Isolation of tissue layers in hermatypic corals by N-acetylcysteine: morphological and proteomic examinations

Cited by 28 publications

References 20 publications

Coral larvae exhibit few measurable transcriptional changes during the onset of coral-dinoflagellate endosymbiosis

Coral larvae exhibit few measurable transcriptional changes during the onset of coral-dinoflagellate endosymbiosis

Diel rhythmicity of lipid-body formation in a coral-Symbiodinium endosymbiosis

Scleractinian coral cell proliferation is reduced in primary culture of suspended multicellular aggregates compared to polyps

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