Isolation of Two Novel WNT Genes, WNT14 and WNT15, One of Which (WNT15) Is Closely Linked to WNT3 on Human Chromosome 17q21

Bergstein, Ivan; Eisenberg, Leonard M.; Bhalerao, Jayant; Jenkins, Nancy A.; Copeland, Neal G.; Osborne, Michael P.; Bowcock, Anne M.; Brown, Anthony M.

doi:10.1006/geno.1997.5041

Cited by 51 publications

(26 citation statements)

References 43 publications

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“…Wnts 4, 5B, 7B and 11 are all expressed during kidney development, but of these only Wnt11 can result in transformation (Christiansen et al, 1996) and b-catenin accumulation (Ku and Melton, 1993). Curiously, human Wnt3 and Wnt15 have both been mapped to within 125 kb on human chromosome 17q21 (Bergstein et al, 1997), a region identi®ed as a familial Wilms' tumour gene, FWT1 (Rahman et al, 1998), although their expression in kidney has not been investigated. Wnt3 does appear to signal via the b-catenin pathway and result in transformation (Shimizu et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Wnt-4 regulation by the Wilms' tumour suppressor gene, WT1

Sim

Smith

et al. 2002

Oncogene

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The Wilms' tumour suppressor gene, WT1, encodes multiple nuclear protein isoforms, all containing four C-terminal zinc ®nger motifs. WT1 proteins can both activate and repress putative target genes in vitro, although the in vivo relevance of these putative target genes is often unveri®ed. WT1 mutations can result in Wilms' tumour and the Denys-Drash Syndrome (DDS) of infantile nephropathy, XY pseudohermaphroditism and predisposition to Wilms' tumour. We have established stable transfectants of the mouse mesonephric cell line, M15, which express WT1 harbouring a common DDS point mutation (R394W). A comparison of the expression pro®les of M15 and transfectant C2A was performed using Nylon-based arrays. Very few genes showed di erential expression. However Wnt-4, a member of the Wnt gene family of secreted glycoproteins, was downregulated in C2A and other similar clones. Doxycycline induction of WT1-A or WT1-D expression in HEK293 stable transfectants also elicited an elevation in Wnt4 expression. Wnt4 is critical for the mesenchyme-to-epithelial transition during kidney development, making it an attractive putative WT1 target. We have mapped human Wnt-4 gene to chromosome 1p35-36, a region of frequent LOH in WT, have characterized the genomic structure of the human Wnt-4 gene and isolated 9 kb of immediate promoter. While several potential WT1 binding sites exist within this promoter, reporter analysis does not strongly support the direct regulation of Wnt4 by WT1. We propose that Wnt-4 regulation by WT1 occurs at a more distant promoter or enhancer site, or is indirect.

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Section: Discussionmentioning

confidence: 99%

Wnt-4 regulation by the Wilms' tumour suppressor gene, WT1

Sim

Smith

et al. 2002

Oncogene

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“…WNT14 partial clone previously reported by another group (22) corresponds to codon 221-343 of WNT14 complete sequence determined in this study (Fig. 2B).…”

Section: Amino Acid Sequence Of Wnt3a and Wnt14mentioning

confidence: 80%

“…Although chicken wnt14 cDNA and human WNT14 partial cDNA are isolated by another group (22), expression profiles were not reported yet. Human WNT14 was moderately expressed in skeletal muscle and heart, and weakly expressed in placenta, adult lung, pancreas, spleen, ovary, fetal brain and fetal lung (Fig.…”

Section: Discussionmentioning

confidence: 98%

“…Human WNT14 gene fragments, corresponding to chicken wnt14 (22), were identified on the same human genome draft sequence that contained the WNT3A gene. Although two additional WNT14 exons were identified on the human genome draft sequence AL360269.3 with the Tblastn program by using chicken wnt14 amino-acid sequence as a query sequence, putative exon 1 of the WNT14 gene was not identified.…”

Section: Isolation Of Wnt14 Cdnasmentioning

confidence: 99%

“…WNT14 and WNT3A genes, consisting of 4 exons, were clustered in the head to head manner with an interval of about 58.0 kb, and were mapped to human chromosome 1q42 region by fluorescence in situ hybridization (FISH). WNT3 and WNT15, clustered in human chromosome 17q21 region (22), are related genes of WNT3A and WNT14, respectively. WNT3A-WNT14 gene cluster and WNT3-WNT15 gene cluster might be generated due to duplication of ancestral gene cluster, just like WNT10A-WNT6 gene cluster and WNT10B-WNT1 gene cluster.…”

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confidence: 99%

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Molecular Cloning and Characterization of WNT3A and WNT14 Clustered in Human Chromosome 1q42 Region

Saitoh¹,

Hirai

Katoh³

2001

Biochemical and Biophysical Research Communications

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Phylogenetic analysis of theWnt gene family and discovery of an arthropodWnt-10 orthologue

Jockusch

Ober

2000

J. Exp. Zool.

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Wnt genes encode a conserved family of secreted signaling proteins that play many roles in arthropod and vertebrate development. We have investigated both the phylogenetic history and molecular evolution of this gene family. We have identified a novel Wnt gene in a diversity of arthropods that it is likely an orthologue of the vertebrate Wnt‐10 group. Wnt‐10 is one of only two cases in which orthology between protostome and deuterostome genes could be consistently assigned based on our analyses. Despite difficulties in assessing orthologies, all of our trees suggest that the most recent common ancestor of protostomes and deuterostomes possessed more than the five Wnt genes known from either arthropods or nematodes. This suggests that Wnt gene loss has occurred during protostome evolution. In addition, we examined the rate of amino acid evolution in the two arthropod/deuterostome orthology groups we identified. We found little rate variation across taxa, with the exception that Drosophila Wnt‐1 is evolving more rapidly than all vertebrate and most arthropod orthologues. J. Exp. Zool. (Mol. Dev. Evol.) 288:105–119, 2000. © 2000 Wiley‐Liss, Inc.

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Isolation of Two Novel WNT Genes, WNT14 and WNT15, One of Which (WNT15) Is Closely Linked to WNT3 on Human Chromosome 17q21

Cited by 51 publications

References 43 publications

Wnt-4 regulation by the Wilms' tumour suppressor gene, WT1

Wnt-4 regulation by the Wilms' tumour suppressor gene, WT1

Molecular Cloning and Characterization of WNT3A and WNT14 Clustered in Human Chromosome 1q42 Region

Phylogenetic analysis of theWnt gene family and discovery of an arthropodWnt-10 orthologue

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