Isonicotinic Acid Hydrazide as Reagent for Determination of Δ⁴-3-Ketosteroids

Stability Assays of Pharmaceutical Preparations by Quantitative Paper Chromatography

“…The absorption spectra of the isonicotinyl hydrazones of testosterone enanthate and 17diycirouy-progesterone caproate are similar to the spectrum given by Umberger (4) for the hydrazone of testosterone with a maximum a t 380 mp. The absorbances of the eluates can be measured in two different ways, both giving equally reliable results (Table VI).…”

“…absorber which has a chromatographic mobility similar to the steroids being assayed. While a clear separation between the steroid and benzyl benzoate is not obtained, its presence is negated by reacting the steroid with isonicotinic acid hydrazide The use of acidified alcoholic isonicotinic acid hydrazitle solution as a colorimetric reagent and as a paper chromatographic spray reagent for the detection of 4-dehydro-3-keto steroids have been dewribed by a number of investigators (4,7,8). The manner in which it is utilized as a simultaneous eluant and colorimetric reagent as reported here has not, to the authors' knowledge, been described previously.…”

“…The high paper blank a t 380 111p is primarily due t o the absorbance of excess hydrazide in the eluates (4).…”

“…Kushner, et al (4), also observed that many compounds containing a benzylamide moiety possessed pronounced anticonvulsm'. activity and their study of a variety of such substances resulted in the development of N-benzyl-0-chloropropionamide.…”

Application of Reverse Phase Partition Chromatography to the Analysis of Testosterone Propionate in Oil Injectables

“…Umberger (7) used an adsorbent magnesium silicate' to chromatographically separate testosterone propionate and progesterone from their oil solution prior to colorimetric determination of their isonicotinylhydrazones. Tappi et al (8) also used the adsorbent magnesium silicate to separate steroids from olive oil.…”