Isotachophoresis and isotachophoresis-zone electrophoresis of food additives on a chip with column-coupling separation channels

Bodor, Róbert; Žúborová, Mária; Ölvecká, Eva; Madajová, V.; Masár, Marián; Kaniansky, Dušan; Stanislawski, Bernd

doi:10.1002/1615-9314(20010901)24:9<802::aid-jssc802>3.0.co;2-1

Cited by 71 publications

(47 citation statements)

References 23 publications

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“…Similarly, an acid (e.g., hydrogen chloride for acid stacking) is used for preconcentrating cationic analytes (Weiss et al 2001). ITP is a commonly used preconcentration method that inserts a large volume of sample with a low concentration between a leading and a terminating electrolyte zone, followed by sharpening the sample zone through the electric field boundaries (Bodor et al 2001). tITP is a simplified form of ITP by adding leading electrolyte (LE) and terminating electrolyte (TE) into the sample buffer and the BGE, respectively (Jeong et al 2005).…”

Section: Dynamic Preconcentration Based On Velocity Differencesmentioning

confidence: 99%

Sample preconcentration in microfluidic devices

Lin

Hsu

Lee

2010

Microfluid Nanofluid

112

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Microfluidic systems have attracted considerable attention and have experienced rapid growth in the past two decades due to advantages associated with miniaturization, integration, and automation. Poor detection sensitivities mainly attributed to the small dimensions of these lab-on-a-chip (LOC) devices; however, sometimes can greatly hinder their practical applications in detecting low-abundance analytes, particularly those in bio-samples. Although off-chip sample pretreatment strategies can be used to address this problem prior to analysis, they may introduce contaminants or lead to an undesirable loss of some original sample volume. Moreover, they are often time-consuming and labor-intensive. Toward the goals of automation, improvement in analytical efficiency, and reductions in sample loss and contamination, many on-chip sample preconcentration techniques based on different working principles for improving the detection sensitivity have been developed and implemented in microchips. The aim of this article is to review recent works in microchipbased sample preconcentration techniques and give detailed discussions about these techniques. We start with a brief introduction regarding the importance of preconcentration techniques in microfluidics and the classification of these techniques based on their concentration mechanisms, followed by in-depth discussions of about these techniques. Finally, personal perspectives on microfluidic-based sample preconcentration will be provided. These advancements in microfluidic sample preconcentration techniques may provide promising strategies for improving the detection sensitivities of LOC devices in many practical applications.

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Section: Dynamic Preconcentration Based On Velocity Differencesmentioning

confidence: 99%

Sample preconcentration in microfluidic devices

Lin

Hsu

Lee

2010

Microfluid Nanofluid

112

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“…Prest and co-workers [75,76] have produced isotachophoresis-chips in silicon rubber and demonstrated the determination of metal cations. Kaniansky and Grass [77][78][79][80][81][82][83][84] with co-workers have reported devices manufactured in poly(methylmethacrylate) (PMMA), which may be employed for isotachophoresis and the coupling of isotachophoresis with zone electrophoresis. The determination of different inorganic and organic ions in samples such as natural water, wine, fruit juices and urine has been reported.…”

Section: Figure 5 Schematic Drawing Of a C 4 Dmentioning

confidence: 99%

Conductimetric and potentiometric detection in conventional and microchip capillary electrophoresis

2002

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Potentiometric detection is rarely used in separation methods but is promising for certain classes of analytes which can only with difficulty be quantified by more standard methods. Conductimetric detection of ions is very versatile and has recently received renewed interest spurned by the introduction of the capacitively coupled contactless configuration. Both are useful and complementary alternatives to the established optical detection methods, and to the more widely known electrochemical method of amperometry. The simplicity of the electrochemical methods makes them particularly attractive for microfabricated devices, but relatively little work has to date been carried out with regard to potentiometric and conductimetric detection.

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“…On the other hand, however, they may adversely affect overall fluctuations of the migration velocities of the separated constituents and, consequently, reproducibilities of their migration times and quantitations. In a general sense, the separations carried out without EOF and HDF offer a better performance in these respects [44][45][46][47][48][49]. Therefore, preferring this transport concept, we performed the ITP-CZE separations in a hydrodynamically closed separation compartment with suppressed EOF.…”

Section: Transport Processes and Itp-cze Separations On The CC Chipmentioning

confidence: 99%

“…A transfer of the CC-CE separation technology to a chip format is a subject of our current research interest [44][45][46][47][48][49]. For example, in this context we showed that the ITP-CZE separations on a poly(methylmethacrylate) CC-CE chip with on-column conductivity detectors provide low ppb concentration detectabilities for some anionic trace constituents that need to be determined in water (nitrite, fluoride and phosphate) also in samples containing anionic macroconstituents (chloride, sulfate, nitrate) at 10 4 higher concentrations [46].…”

Section: Introductionmentioning

confidence: 99%

Determination of bromate in drinking water by zone electrophoresis-isotachophoresis on a column-coupling chip with conductivity detection

et al. 2002

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The use of capillary zone electrophoresis (CZE) on-line coupled with isotachophoresis (ITP) sample pretreatment (ITP-CZE) on a poly(methylmethacrylate) chip, provided with two separation channels in the column-coupling (CC) arrangement and on-column conductivity detection sensors, to the determination of bromate in drinking water was investigated. Hydrodynamic and electroosmotic flows of the solution in the separation compartment of the chip were suppressed and electrophoresis was a dominant transport process in the ITP-CZE separations. A high sample load capacity, linked with the use of ITP in this combination, made possible loading of the samples by a 9.2 microL sample injection channel of the chip. In addition, bromate was concentrated by a factor of 10(3) or more in the ITP stage of the separation and, therefore, its transfer to the CZE stage characterized negligible injection dispersion. This, along with a favorable electric conductivity of the carrier electrolyte solution, contributed to a 20 nmol/L (2.5 ppb) limit of detection for bromate in the CZE stage. Sample cleanup, integrated into the ITP stage, effectively complemented such a detection sensitivity and bromate could be quantified in drinking water matrices when its concentration was 80 nmol/L (10 ppb) or slightly less while the concentrations of anionic macroconstituent (chloride, sulfate, nitrate) in the loaded sample corresponding to a 2 mmol/L (70 ppm) concentration of chloride were still tolerable. The samples containing macroconstituents at higher concentrations required appropriate dilutions and, consequently, bromate in these samples could be directly determined only at proportionally higher concentrations.

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Isotachophoresis and isotachophoresis-zone electrophoresis of food additives on a chip with column-coupling separation channels

Cited by 71 publications

References 23 publications

Sample preconcentration in microfluidic devices

Sample preconcentration in microfluidic devices

Conductimetric and potentiometric detection in conventional and microchip capillary electrophoresis

Determination of bromate in drinking water by zone electrophoresis-isotachophoresis on a column-coupling chip with conductivity detection

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