Isotonic Solutions

Grosicki, Thaddeus S.; Husa, William J.

doi:10.1002/jps.3030431018

Cited by 26 publications

(8 citation statements)

References 8 publications

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“…[8][9][10][11][12][13][14][15][16] It is clear that most of these methods use a large formulation:blood ratio and long contact time to evaluate hemolysis. It has previously been determined that hemolysis increases with both the formulation:blood ratio 12,17 and contact time, [16][17][18][19][20] so the degree of hemolysis determined by these methods will be larger than the amount occurring following an iv injection.…”

Section: Introductionmentioning

confidence: 99%

Lysis of Human Red Blood Cells. 4. Comparison of in Vitro and in Vivo Hemolysis Data

Krzyzaniak¹,

Núñez²,

Raymond³

et al. 1997

Journal of Pharmaceutical Sciences

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The dynamic in vitro method developed by the authors and other available in vitro methods were used to determine the degree of hemolysis induced by several cosolvent vehicles that have previously been evaluated in vivo. The in vitro data generated for each of these vehicles was compared with the in vivo hemolysis data to assess the ability of the method to estimate in vivo hemolysis. The results show that the in vitro data generated by the dynamic method are in agreement with the in vivo data for each vehicle. Therefore, the potential for formulations to induce intravascular hemolysis after injection can be determined by this dynamic in vitro method. With this information, hemolytically safe formulations can more easily be prepared.

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Section: Introductionmentioning

confidence: 99%

Lysis of Human Red Blood Cells. 4. Comparison of in Vitro and in Vivo Hemolysis Data

Krzyzaniak¹,

Núñez²,

Raymond³

et al. 1997

Journal of Pharmaceutical Sciences

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“…Hemolysis of Human Erythrocytes-The method made use of colorimetric hemoglobin determinations to indicate the degree of hemolysis. It was essentially the same as that employed by Husa et al (9,10) as modified by Hamrnarlund and Van Pevenage (3). The percent of hemolysis found for the 63 compounds studied is listed in Table I1 in addition to the isosmotic concentration used for each and the solution's approximale pH.…”

Section: Experimental and Resultsmentioning

confidence: 92%

Sodium Chloride Equivalents, Cryoscopic Properties, and Hemolytic Effects of Certain Medicinals in Aqueous Solution II: Supplemental Values

Fassett¹,

Fuller²,

Hammarlund³

1969

Journal of Pharmaceutical Sciences

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0 A supplemental table of NaCl equivalents and freezing point depressions at various concentrations for 30 different medicinal substances in aqueous solution is presented. Also given in the table is the isosmotic concentration of each of the materials which can form a solution. The degree of hemolysis of human erythrocytes was determined in 63 different isosmotic solutions and the data are presented in a table to supplement the previously published values.Freezing point depression graphs for two substances-fluphenazine dihydrochloride and methotrimeprazine hydrochloride-are presented because they showed discontinuities caused by some type of association or aggregation in their solutions.Keyphrases 0 Sodium chloride equivalents-drugs 0 Cryoscopic properties4rugs 0 Hemolytic effects-drugs 0 Colorimetric analysis-hemolysis determination

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“…Collection of Blood.-The rabbit and human (Caucasian) blood samples employed in this investigation were obtained in the manner described by Grosicki and Husa (9). Caucasian blood was consistently employed to avoid the osmotic differences found in blood samples from Negro donors (8).…”

Section: Methodsmentioning

confidence: 99%

“…It has been shown, in uitro and in uiuo, that certain chemicals in amounts calculated to be isoosmotic to the red blood cell according to physicochemical data may pass through the erythrocytic membrane and fail to prevent osmotic hemolysis (2)(3)(4)(5)(6)(7)(8)(9). In addition, certain substances may be cytotoxic to the erythrocyte, thus altering the integrity of the membrane and resulting in the escape and/or denaturation of the cell contents.…”

mentioning

confidence: 99%