Itaconic acid production using sago starch hydrolysate by Aspergillus terreus TN484-M1

Dwiarti, Lies; Otsuka, Megumi; Miura, Shigenobu; Yaguchi, Masaaki; Okabe, Mitsuyasu

doi:10.1016/j.biortech.2006.03.016

Cited by 63 publications

(43 citation statements)

References 14 publications

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“…IA, also known as 2-methylidenebutanedioic acid, is an unsaturated dicarboxylic acid with conjugated double bonds (Blumhoff et al, 2013). Owing to its valuable chemical properties, IA has been used as an important raw material for the synthesis of fiber, resin, plastic, rubber, paint, surfactants, ion-exchange resins, and lubricants (Dwiarti et al, 2007). In addition, valuable chemicals derived from fossil fuels, such as acrylic acid, acetone cyanohydrin, maleic anhydride, and sodium tripolyphosphate, could be replaced by IA (Pomogailo et al, 2010;Shang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Itaconic acid production from glycerol using Escherichia coli harboring a random synonymous codon‐substituted 5′‐coding region variant of the cadA gene

Jeon

Cheong

Han

et al. 2016

Biotech & Bioengineering

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Aspergillus terreus cadA, encoding cis-aconitate decarboxylase, is an essential gene for itaconic acid (IA) biosynthesis, but it is primarily expressed as insoluble aggregates in most industrial hosts. This has been a hurdle for the development of recombinant strategies for IA production. Here, we created a library of synonymous codon variants (scv) of the cadA gene containing synonymous codons in the first 10 codons (except ATG) and screened it in Escherichia coli. Among positive clones, E. coli scvCadA_No8 showed more than 95% of expressed CadA in the soluble fraction, and in production runs, produced threefold more IA than wild-type E. coli in Luria-Bertani broth supplemented with 0.5% glucose. In M9 minimal media containing 0.85 g/L citrate and 1% glycerol, E. coli scvCadA_No8 produced 985.6 ± 33.4 mg/L IA during a 72-h culture after induction with isopropyl β-D-1-thiogalactopyranoside. In a 2-L fed-batch fermentation consisting of two stages (growth and nitrogen limitation conditions), we obtained 7.2 g/L IA by using E. coli by introducing only the scv_cadA gene and optimizing culture conditions for IA production. These results could be combined with metabolic engineering and generate an E. coli strain as an industrial IA producer. Biotechnol. Bioeng. 2016;113: 1504-1510. © 2015 Wiley Periodicals, Inc.

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Section: Introductionmentioning

confidence: 99%

Itaconic acid production from glycerol using Escherichia coli harboring a random synonymous codon‐substituted 5′‐coding region variant of the cadA gene

Jeon

Cheong

Han

et al. 2016

Biotech & Bioengineering

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“…Studies showed that the temperature of itaconic acid fermentation occurred in the range of 30 °C to 40 °C (Gyamerah 1995;Dwiarti et al 2007). Table 1 examines the effects of initial glucose concentration and temperature on itaconic acid production by A. terreus CICC 2452.…”

Section: Effects Of the Initial Glucose Concentration And Temperaturementioning

confidence: 99%

Improved Itaconic Acid Production from Undetoxified Enzymatic Hydrolysate of Steam-Exploded Corn Stover using an Aspergillus terreus Mutant Generated by Atmospheric and Room Temperature Plasma

Li¹,

Zheng²,

Lai³

et al. 2016

BioResources

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Itaconic acid production by Aspergillus terreus (A. terreus) was investigated using the undetoxified enzymatic hydrolysate of steamexploded corn stover as the sole carbon source. The fermentation conditions for A. terreus were optimized based on glucose as the carbon source. Unfortunately, wild-type A. terreus did not grow in the undetoxified enzymatic hydrolysate. Therefore, atmospheric and room temperature plasma (ARTP) mutagenesis was applied to obtain A. terreus mutant AT-90. A. terreus mutant AT-90 grew and secreted itaconic acid in the undetoxified enzymatic hydrolysate. The highest itaconic acid concentration (19.30 g/L) with a yield of 36.01% was obtained from the undetoxified enzymatic hydrolysate of 10% (w/v) steam-exploded corn stover. This work demonstrated that the A. terreus mutant generated by ARTP efficiently improved itaconic acid production from lignocellulosebased carbon source.

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“…Proposals for the use of residual biomasses, which involved adding fermentative media enriched with nutrients, and aimed at an optimized use of supplements, obtained rates of productivity ranging from 0.1 to 40.0 g IA L −1 [27,28]. In our study, since there was a need to enhance the viability of the bioconversion, as well as to overcome the scale-up difficulties found in many proposals-caused by the high cost of the supplements-it was decided to avoid the use of supplements, even though it would result in a lower productivity.…”

Section: Introductionmentioning

confidence: 99%

Building block itaconic acid from left-over biomass

Pedroso

Montipó

Mario

et al. 2016

Biomass Conv. Bioref.

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The use of rice husks for the sustainable production of the priority building block itaconic acid was studied under multivariate conditions. With the use of 3.4 % (v v −1 ) H 3 PO 4 , 145°C (48 bar), and 28-min hydrolysis time, 44.4 g sugar L −1 was produced with a yield of 266.4 mg sugar g −1 rice husks. Using 3.8 % (v v −1 ) HNO 3 , the best hydrolysis condition was 135°C (45 bar) and 35-min reaction time, when 42.0 g sugar L −1 was produced with a yield of 252.0 mg sugar g −1 rice husks. This is higher than the corresponding results of those obtained in the literature. The fermentation was conducted under optimized multivariate conditions, at 30°C, using phosphoric hydrolysate and an addition of 10 g L −1 yeast extract, pH 6.0 adjusted with CaO (s) and the use of Aspergillus terreus (ATCC 10020). Besides using no supplementation, the optimized experiment produced 1.9 g itaconic acid L −1 (yield of 49 mg g −1 sugar), similar results to that obtained with supplementation found in the literature for other kinds of lignocellulosic materials.

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Itaconic acid production using sago starch hydrolysate by Aspergillus terreus TN484-M1

Cited by 63 publications

References 14 publications

Itaconic acid production from glycerol using Escherichia coli harboring a random synonymous codon‐substituted 5′‐coding region variant of the cadA gene

Itaconic acid production from glycerol using Escherichia coli harboring a random synonymous codon‐substituted 5′‐coding region variant of the cadA gene

Improved Itaconic Acid Production from Undetoxified Enzymatic Hydrolysate of Steam-Exploded Corn Stover using an Aspergillus terreus Mutant Generated by Atmospheric and Room Temperature Plasma

Building block itaconic acid from left-over biomass

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