ITS1 intra-individual variability of Ascaris isolates from Brazil

Paleoparasitological report on Ascaris aDNA from an ancient East Asian sample

“…Therefore, the aDNA analysis reported here falls short of confirming the Ascaris aDNA as A. lumbricoides. Many currently available nuclear or mitochondrial targets are not satisfactory for differentiating A. lumbricoides from A. suum (Anderson 2001, Peng et al 2005, Leles et al 2010, which stems from the close relationship between the two species (Barry & O' Rourke 1967, Crompton 1989, Zhu et al 1999, Leles et al 2010. This is again evident in our study because the differentiation between these two species was not achieved after genetic analysis.…”

“…In the case of ancient Ascaris spp, one of the most widespread parasitic infections in humans (Loreille & Bouchet 2003), PCR-based analyses have been performed and reported by Loreille et al (2001) and Leles et al (2008). However, even though Ascaris spp have been identified by molecular studies of archaeological specimens, their genetic diagnosis has not been studied in greater detail so far (Anderson 2001, Peng et al 2005, Leles et al 2010. Moreover, since most aDNA studies on Ascaris have been limited in their geographical distribution to Europe and South America, it is desirable to secure Ascaris aDNA data from a much wider geographic and temporal range.…”

Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections

“…Several studies using different markers showed zoonotic potential of Ascaris, as humans and pigs were found to share common haplotypes. Although some researchers leave the taxonomic status undefined, Leles et al concluded that based on nematode crossinfections between humans and pigs, the hybridization between A. lumbricoides and A. suum, and the high levels of genetic similarity between the complete mtDNA genomes, there is a single interbreeding population of Ascaris, and those authors recommended synonymizing the two species with the name A. lumbricoides Linnaeus 1758 (326). The codon 200 TAC SNP in the ␤-tubulin gene was not found in any of the A. lumbricoides samples (n ϭ 38) tested by using a pyrosequencing assay for the detection of SNPs in this gene that are associated with benzimidazole resistance (316).…”

Genetic diversity of ITS sequences of Bursaphelenchus xylophilus

“…Intraspecific diversity between virulent and avirulent B. xylophilus isolates has been detected using RFLP analysis with the enzyme HhaI (Aikawa et al, 2003(Aikawa et al, , 2006. ITS genetic diversity has been reported within species and individuals from a diverse range of eukaryotes, including insects (Fairley et al, 2005;Li and Wilkerson, 2007), marine sponges (Worheide et al, 2004;Vierna et al, 2010), fungi (Horton, 2002;Smith et al, 2007), and nematodes such as Meloidogyne (Hugall et al, 1999), Brugia (Areekit et al, 2009), Ascaris (Leles et al, 2010), and Pratylenchus (De Luca et al, 2011). However, to our knowledge, no data on ITS intra-isolate and intra-individual diversity in B. xylophilus or other Bursaphelenchus species have been published.…”