Iturins, a special class of pore-forming lipopeptides: biological and physicochemical properties

Maget‐Dana, Régine; Peypoux, Françoise

doi:10.1016/0300-483x(94)90159-7

Cited by 360 publications

(224 citation statements)

References 75 publications

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“…The spots I, II and III might be isoforms of fengycins as their Rf values on TLC and HPLC profile (Rt 6.87 min) were similar to those earlier reported in literature (Yanez-Mendizabel et al, 2012;Grover et al, 2010). These amphiphilic cyclic lipopeptide (CLP) compounds have been reported for their wide spectrum antifungal activity due to their interaction with biological membranes (Magnet-Dana & Peypoux, 1994;Stein, 2005). The TLC-based bioautography studies indicated varied antifungal potential of these biomolecules with the respective fungal pathogen.…”

Section: Discussionsupporting

confidence: 84%

Antifungal potential of Bacillus vallismortis R2 against different phytopathogenic fungi

Kaur

Saini

2015

Span. j. agric. res.

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The cash crops grown in an agro-climatic region are prone to infection by various fungal pathogens. The use of chemical fungicides over the years has resulted in emergence of resistant fungal strains, thereby necessitating the development of effective and environmental friendly alternatives. The natural antagonistic interactions among different microbial populations have been exploited as an eco-friendly approach for controlling fungal pathogens resistant to synthetic chemicals. Morphologically distinct bacterial cultures (150), isolated from rhizospheric soils of wheat, rice, onion and tomato plants were screened for their antifungal potential against seven phytopathogenic fungi prevalent in the State of Punjab (India). The bacterial isolate R2, identified as Bacillus vallismortis, supported more than 50% inhibition of different phytopathogenic fungi (Alternaria alternata, Rhizoctonia oryzae, Fusarium oxysporum, Fusarium moniliforme, Colletotrichum sp, Helminthosporium sp and Magnaporthe grisea) in dual culture plate assay. The thin layer chromatography based bio-autography of acid-precipitated biomolecules (APB) indicated the presence of more than one type of antifungal molecule, as evidenced from zones of inhibition against the respective fungal pathogen. The initial analytical studies indicated the presence of surfactin, iturin A and fengycin-like compounds in APB. The antifungal activity of whole cells and APB of isolate R2 was evaluated by light and scanning electron microscopy. The wheat grains treated with APB and exposed to spores of A. alternata showed resistance to the development of black point disease, thereby indicating the potential application of R2 and its biomolecules at field scale level.

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Section: Discussionsupporting

confidence: 84%

Antifungal potential of Bacillus vallismortis R2 against different phytopathogenic fungi

Kaur

Saini

2015

Span. j. agric. res.

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“…Some hemolytic activities have been detected in strains producing antibiotic compounds such as bacteriocins, iturin A, surfactin, or mycosubtilin (Hsieh et al, 2004;Leclère et al, 2005). The specific mechanisms of these antibiotics are diverse, but the formation of pores in erythrocytes, as well as in bacterial or fungal cell walls, is a predominant mechanism (Maget-Dana and Peypoux, 1994). Therefore, strains producing these kinds of antibiotics contain an excellent activity to inhibit the growth of competitors in ecosystems like the rhizosphere.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of genetic variability in bacteria with β-hemolytic and antifungal activity isolated from the rhizosphere of Medicago truncatula plants

Hernández-Salmerón¹,

Prieto-Barajas²,

Valencia-Cantero³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. In the present study, we analyzed the frequency of hemolytic and antifungal activities in bacterial isolates from the rhizosphere of Medicago truncatula plants. Of the 2000 bacterial colonies, 96 showed β-hemolytic activities (frequency, 4.8 x 10 -2 ). Hemolytic isolates were analyzed for their genetic diversity by using random amplification of polymorphic DNA, yielding 88 haplotypes. The similarity coefficient of Nei and Li showed a polymorphic diversity ranging from 0.3 to 1. Additionally, 8 of the hemolytic isolates showed antifungal activity toward plant pathogens, Diaporthe phaseolorum, Colletotrichum acutatum, Rhizoctonia solani, and Fusarium oxysporum. The 16S ribosomal sequencing analysis showed that antagonistic bacterial isolates corresponded to Bacillus subtilis (UM15, UM33, UM42, UM49, UM52, and UM91), Bacillus pumilus (UM24), and Bacillus licheniformis (UM88). The present results revealed a higher genetic diversity among hemolytic isolates compared to that of isolates with antifungal action.

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“…The antagonistic effects of the iturin cyclic peptides are the result of their interaction with the cell membrane and formation of pores (Maget-Dana and Peypoux, 1994). The Tyr residue at position 2 in the peptide ring of peptides from the iturin family has a significant role in the mechanism of pore formation in target cells (Harnois et al, 1989, Volpon et al, 1999.…”

Section: Mode Of Action Of Bacillomycins Against Plant Pathogensmentioning

confidence: 99%

“…The Tyr residue at position 2 in the peptide ring of peptides from the iturin family has a significant role in the mechanism of pore formation in target cells (Harnois et al, 1989, Volpon et al, 1999. The antimicrobial activity of iturins depends predominantly on its capacity to increase membrane permeabilization, which is being attributed to aggregates formed by iturin molecules, iturinphospholipid complex or iturin-phospholipidsterol complex (Maget-Dana and Peypoux, 1994). The underlying mechanism is based on osmotic perturbation owing to the formation of ion-conducting pores and not membrane disruption or solubilization as caused by surfactins (Aranda et al, 2005).…”

Section: Mode Of Action Of Bacillomycins Against Plant Pathogensmentioning

confidence: 99%

Bacillomycins – The Effective Molecules in Plant Disease Management

Suneeta¹,

Aiyanathan²,

Nakkeeran³

2018

Int.J.Curr.Microbiol.App.Sci

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Iturins, a special class of pore-forming lipopeptides: biological and physicochemical properties

Cited by 360 publications

References 75 publications

Antifungal potential of Bacillus vallismortis R2 against different phytopathogenic fungi

Antifungal potential of Bacillus vallismortis R2 against different phytopathogenic fungi

Isolation and characterization of genetic variability in bacteria with β-hemolytic and antifungal activity isolated from the rhizosphere of Medicago truncatula plants

Bacillomycins – The Effective Molecules in Plant Disease Management

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