Janibacter cremeus sp. nov., an actinobacterium isolated from sea sediment

Abstract

A novel Gram-stain-positive actinobacterium, designated HR08-44(T), was isolated from a sea-sediment sample collected from the foreshore of Rishiri Island, Japan, and its taxonomic position was investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain HR08-44(T) was closely related to the members of the genus Janibacter, with pairwise sequence similarities of 97.3-98.8 %. Strain HR08-44(T) had peptidoglycan type A1γ, with meso-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-8(H4) and the major fatty acids were iso-C16 : 0, C17 : 1ω8c, C17 : 0 and C18 : 1ω9c. These data supported the affiliation of the novel strain to the genus Janibacter. Meanwhile, the results of DNA-DNA hybridization and physiological and biochemical tests indicated that strain HR08-44(T) can be distinguished from recognized species of the genus Janibacter. Therefore, strain HR08-44(T) represents a novel species of the genus Janibacter, for which the name Janibacter cremeus sp. nov. is proposed; the type strain is HR08-44(T) ( = NBRC 107693(T) = DSM 26154(T)).

Janibacter indicus sp. nov., isolated from hydrothermal sediment of the Indian Ocean

“…S2). The presence of DPG, PG and PI was consistent with analyses of other species of the genus Janibacter (Hamada et al , 2013; Martin et al , 1997; Yoon et al , 2000). The major fatty acids of strain 0704P10-1 T were iso-C 16 : 0 (47.21 %), C 17 : 1 ω8 c (12.45 %) and 10-methyl C 17 : 0 (10.43 %) (Table S1).…”

“…The genus Janibacter was originally proposed by Martin et al (1997) as a member of the family Intrasporangiaceae with the type species Janibacter limosus . At the time of writing, the genus comprises eight species with validly published names: Janibacter limosus (Martin et al , 1997), J. terrae (Yoon et al , 2000), J. melonis (Yoon et al , 2004), J. anophelis (Kämpfer et al , 2006), J. corallicola (Kageyama et al , 2007), J. hoylei (Shivaji et al , 2009), J. alkaliphilus (Li et al , 2012) and J. cremeus (Hamada et al , 2013). Janibacter brevis , originally described by Imamura et al (2000), has since been recognized as a heterotypic synonym of J. terrae (Lang et al , 2003).…”

“… Janibacter brevis , originally described by Imamura et al (2000), has since been recognized as a heterotypic synonym of J. terrae (Lang et al , 2003). Members of the genus Janibacter were isolated from various sources, such as environmentally polluted samples (Martin et al , 1997; Yoon et al , 2000), an air sample (Shivaji et al , 2009), melon (Yoon et al , 2004), the midgut of mosquitoes (Kämpfer et al , 2006), coral (Li et al , 2012) and sea sediment (Hamada et al , 2013).…”

Biodeterioration Risk Threatens the 3100 Year Old Staircase of Hallstatt (Austria): Possible Involvement of Halophilic Microorganisms

“…The DNA profiles obtained allow discrimination at the subspecies level, based on the DNA diversity in the entire bacterial genome, offering a broad spectrum of genetic variation [ 17 , 40 ]. Sequencing results showed that most of the isolated strains were moderately halophilic bacteria growing optimally in media containing 3–10% (w/v) salt, as the species of Idiomarina , Psychrobacter [ 41 ], Kocuria [ 42 ] Staphylococcus , Virgibacillus [ 43 ] Planococcus [ 44 ] and Janibacter [ 45 ], and some species of the genera Bacillus . Interestingly, some strains were isolated only in media containing the highest NaCl concentrations (10–20% (w/v) NaCl), as those related to species of Halovibrio , Salicola , Marinococcus and some species of Bacillus [ 46 ].…”

Whole Genome Sequencing of First Janibacter indicus Isolate in China Revealed Three Unique Genomic Islands Compared with Saprophytic Strains

“…Until now, Janibacter could be divided into nine strain types: J. limosus, J. terrae, J. indicus, J. melonis, J. anophelis, J. hoylei, J. corallicola, J. alkaliphilus, and J. cremeus. [5][6][7][8][9][10][11][12][13] J. indicus was first isolated by Zhang et al in the Indian Ocean. The phylogenetic tree of J. indicus and other Janibacter strains was constructed by 16s rRNA, and revealed that J. indicus is related to J. terrae, J. cremeus, J. anophelis, J. hoylei, J. limosus, J. corallicola, J. melonis, and J. alkaliphilus (Zhang et al).…”