Jasmonates and Na‐orthovanadate promote resveratrol production in Vitis vinifera cv. Barbera cell cultures

Abstract

Summary• Here the effect of jasmonic acid, methyljasmonate and Na-orthovanadate on the production of resveratrol was studied in Vitis vinifera cv. Barbera cell suspension cultures.• Na-orthovanadate at 0.1 m M and 1 m M concentration was efficient in promoting the production and/or accumulation and release in the culture medium of cisresveratrol while trans -resveratrol levels were not affected by this treatment.• Methyljasmonate was highly effective in stimulating both trans -and cis -resveratrol endogenous accumulation, as well as their release into the culture medium. Cisresveratrol was absent or detected in very low amounts in the controls. Jasmonic acid was less efficient than methyljasmonate in promoting endogenous resveratrol accumulation, but it stimulated the release in the culture medium especially of cis -resveratrol.• Gel analysis was performed on control and 10 µ M MeJA treated cell suspensions. Results showed an up-regulation of the stilbene synthase demonstrating that MeJA stimulated the synthesis ex-novo of this protein.

Regulation of stilbene biosynthesis in plants

“…There are multiple studies showing that stilbene production in cultivated grapevine and grapevine cell cultures increased after treatments with JA or MeJA (Larronde et al 2003;Tassoni et al 2005Tassoni et al , 2012Vezzulli et al 2007;Kiselev et al 2007;Faurie et al 2009;Taurino et al 2015;Xu et al 2015;Degu et al 2016). The effects of 12-oxophytodienoic acid (OPDA), a biologically active JA precursor, and coronatine (COR), a fungal phytotoxin mimicking the biologically active form of JA (JA-Ile), on stilbene biosynthesis have been investigated using cell suspension cultures of V. vinifera and compared with that effects of JA and MeJA (Taurino et al 2015;Almagro et al 2015).…”

Biosynthesis and bioactivity of glucosinolates and their production in plant in vitro cultures

“…One of these strategies is the use of elicitors, particularly jasmonic acid and its derivative, methyl jasmonate, which are signal molecules able to increase plant defense responses such as the production of secondary metabolites (Tassoni et al 2005;Almagro et al 2014;Kastell et al 2013a;Alvarez et al 2008). In this sense, Alvarez et al (2008) observed that the treatment with methyl jasmonate provoked an increase of total levels of glucosinolates, and the response was dependent on the age of cell culture.…”

Effect of UV Light on Secondary Metabolite Biosynthesis in Plant Cell Cultures Elicited with Cyclodextrins and Methyl Jasmonate

“…Almagro et al, (2010) demonstrated that the maximum level of ajmalicine produced by cells and secreted to the media was reached when cell suspensions were incubated in the presence of MJ and CDs, production being around 2.2-fold higher than when cells were treated only with CDs. Moreover, Tassoni et al, (2005) showed that MJ was highly effective in stimulating endogenous trans-resveratrol accumulation, as well as promoting its release into the extracellular medium of V. vinifera cv Barbera cell cultures. In this case, the endogenous trans-resveratrol accumulation was around 24 µg/g dry weight (DW) and the transresveratrol secreted to the medium was over 8 µg/g DW.…”