Jejuia pallidilutea gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from seawater

Abstract

related strains were all less than 93.8 %). Cells of strain EM39 T were non-gliding, catalase-and oxidase-positive rods that were devoid of flexirubin pigments. Growth was observed at 15-35 6C (optimum, 25-30 6C) and pH 6.5-9.0 (optimum, pH 7.0-8.5). The genomic DNA G+C content was 34.6 mol% and the major respiratory quinone was MK-6. The predominant cellular fatty acids were iso-C 15 : 0 , iso-C 15 : 1 G and iso-C 17 : 0 3-OH. On the basis of phenotypic and genotypic data, strain EM39 T represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Jejuia pallidilutea gen. nov., sp. nov. is proposed. The type strain is EM39The family Flavobacteriaceae (Reichenbach, 1989;Bernardet et al., 2002) is one of the major phylogenetic lineages within the phylum 'Bacteroidetes' (Garrity & Holt, 2001). The family contains a well-defined 'marine clade', members of which are widespread in marine habitats where they contribute to the mineralization of primary-produced organic matter. They have been isolated from seawater and ice, quartz or basalt stones, marine sediment and algae (Bowman & Nichols, 2005; Einen & Øvreås, 2006; Jung et al., 2005;Kwon et al., 2006a, b;Lee, 2007;Nedashkovskaya et al., 2006Nedashkovskaya et al., , 2007Yang & Cho, 2008). In the course of investigations of bacterial communities in seawater, a novel Gram-stain-negative bacterium that produced pale orange pigments was isolated and investigated using a polyphasic approach. This organism is described below as a representative of a novel species in a new genus within the family Flavobacteriaceae. Strain EM39T was isolated from a surface seawater sample collected off the eastern coast of Jeju Island (33 u 269 N 126 u 549 E) in Korea by using a standard serial dilution plating method and incubation on marine agar 2216 (MA; Difco) at 25 u C for 5 days. Subcultivation was performed routinely on MA at 30 u C for 3 days under aerobic conditions and the strain was stored at 280 u C in marine broth (MB; Difco) supplemented with 10 % (v/v) glycerol for preservation.Sequencing of the 16S rRNA gene of strain EM39T was carried out as described previously (Lane, 1991). The resulting 16S rRNA gene sequence was compared with those from GenBank using the program BLAST (http:// www.ncbi.nlm.nih.gov/BLAST/) to determine an approximate phylogenetic affiliation and then aligned with sequences of closely related organisms by using CLUSTAL W software (Thompson et al., 1994). Phylogenetic trees were constructed by using the neighbour-joining, maximum-likelihood and maximum-parsimony algorithms available in PHYLIP version 3.6 (Felsenstein, 2002). The resulting tree topologies were evaluated using bootstrap Abbreviation: ASW, artificial seawater.

Arenitalea lutea gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from intertidal sand

“…Aquimarina addita JC2680 T (HM475137) with the one formed by the genera Hyunsoonleella (Yoon et al, 2010) and Jejuia (Lee et al, 2009), within a cluster encompassing the genera Hyunsoonleella, Jejuia and Marinivirga and two species of the genus Algibacter (Park et al, 2013b). Topologies of the maximum-parsimony and maximum-likelihood trees were nearly identical to the one shown by the neighbour-joining tree (Figs S1 and S2 available in IJSEM Online).…”

Pontirhabdus pectinivorans gen. nov., sp. nov., isolated from seawater

“…Extraction of fatty acid methyl esters and separation by GC were performed by using the Instant FAME method of the Microbial Identification System (MIDI) version 6.1 and the RTSBA6 6.10 database. The fatty acids were iso-C 15 : 0 3-OH (16.7 %), iso-C 15 : 1 G (14.7 %), iso-C 17 : 0 3-OH (10.7 %), anteiso-C 15 : 0 (7.7 %), iso-C 15 : 0 (7.6 %), iso-C 16 : 0 3-OH Taxa: 1, Strain JC2675 T (data from this study); 2, Algibacter (n52; all data from this study except the DNA G+C content); 3, Lacinutrix (n53; all data from this study except the DNA G+C content); 4, Hyunsoonleella jejuensis CNU004 T ; 5, Jejuia pallidilutea EM39 T (Lee et al, 2009); 6, Olleya (n52; Lee et al, 2010;Nichols et al, 2005); 7, Flaviramulus basaltis H35 T (Einen & Ovreås, 2006). n, Number of type strains in each genus; +, positive; -, negative; W, weakly positive; ND, no data available; V, varies among different strains within the same genus; VA, variable results depending on test methods; tr, trace.…”

Seonamhaeicola algicola sp. nov., a complex-polysaccharide-degrading bacterium isolated from Gracilaria blodgettii, and emended description of the genus Seonamhaeicola

“…Growth occurs at 10-40 uC (optimum, 30-37 uC), at pH 6.0-8.0 (optimum, 7.0-7.5) and with 0-7.0 % (w/v) NaCl (optimum, 2.0-2.5 % Park et al (2014) and Lee et al (2009).…”