Jian-Pi-Bu-Xue-Formula Alleviates Cyclophosphamide-Induced Myelosuppression via Up-Regulating NRF2/HO1/NQO1 Signaling

Abstract

Jian-pi-bu-xue-formula (JPBXF), a TCM formula composed of twelve Chinese medicinal herbs, has been used in clinic to ease patients' state of weakness and fatigue especially after receiving anti-tumor chemotherapy in China. The lack of the phytochemical characterization, detail therapeutic evaluation and mechanism of JPBXF remains the main limitation for its spreading. In this study, we systematically evaluated the effectiveness and underline mechanism of JPBXF on cyclophosphamide (CTX)-induced myelosuppression and identified the main constituents of JPBXF aqueous extract. JPBXF treatments reversed CTX-induced myelosuppression through increasing the number of haematopoietic stem cells (HSCs) and expression of C-kit in bone marrow cells. Simultaneously, JPBXF treatments alleviated CTX-induced blood cells reduction by increasing numbers of RBCs and WBCs and levels of GM-CSF, TPO and EPO in plasma. JPBXF treatments reduced CTX-induced immunosuppression by increasing expressions of CD3, CD4, and CD8a in PBMCs, and recovering structure damages of thymus and spleen. Moreover, JPBXF notably increased the expression of NRF2 compared with CTX group, and subsequently up-regulated HO1 and NQO1 both in mRNA and protein levels. In addition, eighteen compounds were recognized from JPBXF aqueous extract and the potential targets of the identified compounds were predicted. Overall, JPBXF can greatly reverse CTX-induced myelosuppression in C57BL/6 mice, especially in improving the blood and immune function through activating NRF2/HO1/ NQO1 signaling pathway, which provides a reliable reference for JPBXF application in clinical. By recognizing eighteen compounds in JPBXF aqueous extract and predicting the underline mechanisms of the identified compounds, our study would provide theoretical guidance for further research of JPBXF.

The Anti-Neuroinflammatory Effect of Fuzi and Ganjiang Extraction on LPS-Induced BV2 Microglia and Its Intervention Function on Depression-Like Behavior of Cancer-Related Fatigue Model Mice

“…TCMs are diffusely applied in Asian countries, especially China, Korea, and Japan (Huang et al, 2020b). Due to the precise efficacy of TCMs and the advancement of its modernization, Chinese medicine is being gradually accepted and applied by Western countries (Dobos and Tao, 2011).…”

Qingfeiyin Decoction Inhibits H1N1 Virus Infection via Modulation of Gut Microbiota and Inflammatory Pathways in a Murine Model

“…To detect the peak intensity, mass spectrometry was conducted in the negative and positive modes under the following conditions: N2 flow of 20 arb, source temperature of 150°C, ion spray voltage (IS) of 2.3 kV, and a scan time of 25 min. The data were analyzed by Peakview software, the parameters were set as follows: mass error <5 ppm, isotope ratio difference <10; library score >70 ( Huang et al, 2020 ).…”

Polyphenol-Rich Ginger (Zingiber officinale) for Iron Deficiency Anaemia and Other Clinical Entities Associated with Altered Iron Metabolism

“…Dry ginger is used in traditional Chinese medicine as a warm herb to promote blood flow, remove blood stasis, and alleviate weakness and fatigue. As such, ginger is used in many blood tonic herbal formulas for treating blood circulation, anaemia, and haemorheological conditions [ 160 , 161 ]. Ginger is also a tonic food recommended for postpartum women during the one month confinement period immediately after delivery for recovery from blood loss.…”