Kaempferol glycosides and cardenolide glycosides, cytotoxic constituents from the seeds of Draba nemorosa (Brassicaceae)

Moon, Surk‐Sik; Rahman, Aziz Abdur; Manir, Maniruzzaman; Ahamed, V.S. Jamal

doi:10.1007/s12272-010-0806-0

Cited by 23 publications

(14 citation statements)

References 19 publications

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“…Kaempferol is a common flavonoid that is found in vegetables, fruits and tea (25,26). Although kaempferol reportedly has anti-proliferative and cytotoxic effects on human lung cancer cells, to the best of our knowledge, no study has previously demonstrated that it can enhance radiosensitivity (27,28). The present study provides evidence that kaempferol has radiosensitization potential for lung cancer in vitro and in vivo.…”

Section: Discussionsupporting

confidence: 52%

Radiosensitization of non-small cell lung cancer by kaempferol

Kuo

Tsai

et al. 2015

Oncology Reports

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Abstract. The aim of the present study was to determine whether kaempferol has a radiosensitization potential for lung cancer in vitro and in vivo. The in vitro radio-sensitization activity of kaempferol was elucidated in A-549 lung cancer cells by using an MTT (3-(4 5-dimethylthiazol-2-yl)-25-diphenyl tetrazolium bromide) assay, cell cycle analysis and clonogenic assay. The in vivo activity was evaluated in the BALB/c nude mouse xenograft model of A-549 cells by hematoxylin and eosin staining and immunohistochemistry, and the tumor volume was recorded. Protein levels of the apoptotic pathway were detected by western blot analysis. Treatment with kaempferol inhibited the growth of A-549 cells through activation of apoptotic pathway. However, the same doses did not affect HFL1 normal lung cell growth. Kaempferol induced G2/M cell cycle arrest and the enhancement of radiation-induced death and clonogenic survival inhibition. The in vivo data showed that kaempferol increased tumor cell apoptosis and killing of radiation. In conclusion, the findings demonstrated that kaempferol increased tumor cell killing by radiation in vitro and in vivo through inhibition of the AKT/PI3K and ERK pathways and activation of the mitochondria apoptosis pathway. The results of the present study provided solid evidence that kaempferol is a safe and potential radiosensitizer. IntroductionLung cancer is the leading cause of cancer-associated mortalities worldwide, with an estimated 1.61 million new cases and 1.38 million mortailies in 2008 (1,2). Approximately 85% of primary lung cancer patients have non-small-cell lung cancer (NSCLC) (3). Surgery is the standard treatment for early-stage NSCLC. However, the majority of patients diagnosed at an advanced stage are unsuitable for surgical resection or extensive mediastinal lymphadenopathy (4,5). Chemo-and radiotherapy are the current standard of care for patients with unresectable advanced NSCLC (6,7). However, there are several limiting factors of chemo-and radiotherapy, including dose tolerance limitation of normal tissue and tumor radioresistance (8). Accordingly, identifying effective agents for enhancing tumor sensitivity to radiation and reducing adverse effects on normal tissues are crucial.Acting as radiosensitizers, drugs can accelerate the killing of cancer cells by increasing the effectiveness of radiation with little effect on normal cells (9). The PI3K/Akt and ERK pathways play an essential role in confirming sensitivity or resistance to radiation by inhibiting a survival pathway that induces cell apoptosis (10,11). Previous studies also showed that treatment with the ERK inhibitor can reduce radioresistance and suppressed activation of PI3K/Akt can promote radiosentization (12,13). Inhibition of growth of cancer cells and the induction of apoptosis are important determinants of the response to anticancer therapy (14).Bioflavonoids, which are the phytochemicals that are abundant in a variety of plants have a vital role in cancer prevention as they can scavenge free radicals (1...

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Section: Discussionsupporting

confidence: 52%

Radiosensitization of non-small cell lung cancer by kaempferol

Kuo

Tsai

et al. 2015

Oncology Reports

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“…The rest of the carbon signals in association with 1 H NMR signals (Table 1) suggested the presence of two pentose residues. The sugar residues were deduced to be ribose and xylose by comparing the 1 H and 13 C NMR data with a reported compound [20], and arabinose was excluded [21]. Further acid hydrolysis of compound 1, confirmed the sugar residues of D-ribose and D-xylose by HPLC chromatography with authentic standard [18].…”

Section: Resultsmentioning

confidence: 91%

Three new phenolic compounds from the leaves of Rosa sericea

Liu

et al. 2013

Fitoterapia

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“…The structures of compounds were established on the basis of extensive analysis of 1 H, 13 C, HMBC, and HSQC NMR experiments as well as comparing with literature data [Figures S1–S10 in supplementary data]. In this way, compounds 1 to 5 were identified as drabanemoroside [30], kaempferol 3- O - α -L-rhamnopyranosyl(1→2)- α -L-rhamnopyranoside [31], kaempferol [32], p -hydroxybenzoic acid [33], and p -coumaric acid [34], respectively.…”

Section: Resultsmentioning

confidence: 99%

“…After chromatographic purification processes we identified three flavonoids, ( 1 ) drabanemoroside, ( 2 ) kaempferol 3- O - α -L-rhamnopyranosyl(1→2)- α -L-rhamnopyranoside, and ( 3 ) kaempferol, and two phenolic compounds, ( 4 ) p -hydroxybenzoic acid and ( 5 ) p- coumaric acid, in EtOAc [30–34]. The kaempferol ( 3 ) is widely distributed on vegetable kingdom, including the genus Smilax [35].…”

Section: Discussionmentioning

confidence: 99%

“…Finally, for the first time in the literature, our study identified the glucoside flavonoids drabanemoroside ( 1 ) and kaempferol 3- O - α -L-rhamnopyranosyl(1→2)- α -L-rhamnopyranoside in the genus Smilax . There are few studies on drabanemoroside biological activities, and some reports about its antitumoral potential are contradictories [30, 39]. Regarding kaempferol 3- O - α -L-rhamnopyranosyl(1→2)- α -L-rhamnopyranoside, no studies about its biological activities have been described yet.…”

Section: Discussionmentioning

confidence: 99%

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Phytochemical and Antinociceptive, Anti‐Inflammatory, and Antioxidant Studies of Smilax larvata (Smilacaceae)

Hirota

Paula

Oliveira

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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The tea of aerial parts of Smilax larvata Griseb. (Smilacaceae) has been ethnopharmacologically used in Southern Brazil due to its anti-inflammatory action. In this study, ethanolic and organic extracts from aerial parts of S. larvata were phytochemically and pharmacologically characterized. The phytochemical analysis of EtOAc extract of S. larvata revealed the presence of three flavonoids, drabanemoroside, kaempferol 3-O-α-L-rhamnopyranosyl(1→2)-α-L-rhamnopyranoside, and kaempferol, the first two being isolated for the first time in this genus, two phenolic compounds p-hydroxybenzoic acid and p-coumaric acid, and alkaloids. In vitro assays demonstrated a potential antioxidant property of SLG. The treatment with SLG induced a significant reduction of the formalin-evoked flinches in rats, an effect reversed by opioid antagonist naloxone. Treatment with SLG also induced a significant increase in the hot plate latency and a decrease of intestinal motility by 45%. No effect was observed over nociceptive responses induced by a TRPA1 agonist mustard oil or over acetic acid-induced writhing in mice. Together, our data suggested that SLG has an in vivo antinociceptive effect, which seems to be associated with the opioid system activation. These findings support previous claims of medical use of Smilax larvata in the treatment of pain conditions.

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Kaempferol glycosides and cardenolide glycosides, cytotoxic constituents from the seeds of Draba nemorosa (Brassicaceae)

Cited by 23 publications

References 19 publications

Radiosensitization of non-small cell lung cancer by kaempferol

Radiosensitization of non-small cell lung cancer by kaempferol

Three new phenolic compounds from the leaves of Rosa sericea

Phytochemical and Antinociceptive, Anti‐Inflammatory, and Antioxidant Studies of Smilax larvata (Smilacaceae)

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