KAJIAN INDUKSI KALUS RUMPUT LAUT Kappaphycus alvarezii UNTUK PRODUKSI EMBRIO SOMATIK

Rajamuddin, Muh. Alias L.; Jaya, Andi Asdar; Ridwan, Ridwan; Suryati, Emma

doi:10.15578/jra.5.2.2010.211-219

Cited by 6 publications

(3 citation statements)

References 5 publications

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“…Thalli segment, callus and fi lament callus were cultured in the laboratory condition according to method described by Reddy et al (2003) and Rajamuddin (2010). Thalli segment was culture to obtain callus using solid agar medium containing growth regulators, while callus was culture in semisolid medium to produce fi lament callus.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of different promoters driving the GFP reporter gene in seaweed Kappaphycus alvarezii

et al. 2016

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Promoter regulates expression level of foreign gene in transgenic organism. This study was performed to select a suitable promoter as the fi rst step towards production of valuable trait-enhanced seaweed by transgenic technology. Green fl uorescent protein (GFP) gene was used as a reporter to determine the activity of promoter in seaweed Kappaphycus alvarezii. GFP gene constructs driven by cytomegalovirus (pCMV-GFP), caulifl ower mosaic virus (pCaMV-GFP), medaka β-actin (pmBA-GFP) and Japanese fl ounder keratin (pJfKer-GFP) promoters were introduced by electroporation method. Electroporation was performed using a gene pulser (BIORAD) with voltage of 300 V, pulse length of 0.5 ms, pulse numbers of 4, and pulse interval of 0.1 s. Promoter activity was determined by analyzing GFP gene expression level using a fl uorescent microscope. The results showed that CMV regulated highest number of fi lament callus (34.10%±1.49) expressing GFP at medium to strong fl uorescence levels. CaMV promoter had relatively similar activity with CMV, but lower number of fi lament callus expressing GFP (10.48%±0.25). mBA promoter drove GFP expression at medium level and similar number of fi lament callus (8.85%±2.31) expressing GFP with CaMV, while JfKer promoter had lowest activity by means in number of fi lament callus expressing GFP (4.79%±0.26) and GFP expression level. PCR analysis for transgenic confi rmation showed a DNA band of PCR product from pCMV-GFP and pCaMV-GFP expressing fi lament callus in the same size (about 0.6 kb) with positive control of plasmid. Thus, CMV and CaMV promoters was an appropriate promoter and foreign gene could be transferred to fi lament callus by electroporation method. Combining this achievement with developing a culture method of fi lament callus to be thallus, stable transgenic breeding in K. alvarezii can be feasible.

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Section: Methodsmentioning

confidence: 99%

Evaluation of different promoters driving the GFP reporter gene in seaweed Kappaphycus alvarezii

et al. 2016

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“…Fase pertumbuhan rumput laut yang dikultur akan dipengaruhi oleh adanya hormon tumbuh tanaman yaitu auksin atau Indole Acetic Acid (IAA) yang terdapat pada pupuk Conway. Rajamudin et al (2010) mengemukakan bahwa, auksin atau Indole Acetic Acid (IAA) berperan dalam prosesproses perkembangan fisiologis tumbuhan seperti pada proses pemanjangan sel dan pembentukan kalus, sehingga hal ini menyebabkan pertumbuhan rumput laut meningkat. Lanjut Melara dan Arias (2009) menyatakan hormon auksin atau Indole Acetic Acid berpengaruh dalam proses inisiasi sel (menempelnya ribosom subunit kecil pada mRNA), merangsang pembentukan kalus, ekspansi sel (pertambahan volume tumbuhan menjadi besar), dan pembentukan akar, serta pada pembentukan tunas.…”

Section: Hasil Dan Pembahasanunclassified

Penggunaan Pupuk Conway Pada Media Kultur Terhadap Pertumbuhan Bibit Rumput Laut Eucheuma cottonii

Nurfajri

Nasmia

2022

J. Mar. Res.

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Rumput laut jenis Eucheuma cottonii adalah salah satu hasil perikanan yang mempunyai nilai ekonomis cukup penting karena mengandung karagenan yang merupakan bahan dasar dalam industri farmasi, makanan, maupun bahan industri lain. Mengingat besarnya permintaan pasar akan kebutuhan rumput laut maka perlu dilakukan usaha untuk meningkatkan produksinya. Namun seiring dengan meningkatnya permintaan, pembudidaya sering mengalami permasalahan antara lain bibit rumput laut yang dihasilkan belum maskimal baik dari segi jumlah maupun kualitas, sehingga perlu ada kajian untuk menghasilkan pertumbuhan benih bibit rumput laut yang baik yaitu menggunakan pupuk Conway pada media kultur rumput laut dari hasil kultur jaringan. Pupuk conway mengandung unsur nutrien yang sangat bermanfaat bagi pertumbuhan rumput laut. Penelitian ini bertujuan untuk mendapatkan dosis pupuk Conway yang tepat pada media kultur terhadap pertumbuhan bibit rumput laut E. cottonii. Penelitian ini menggunakan E.cottoni dari hasil kultur jaringan Seameo Biotrop. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 5 perlakuan dan 5 ulangan yaitu dosis pupuk Conway (0, 2, 4, 6, dan 8 ml). Padat tebar E.cottoni yaitu 5 eksplan per wadah. Hasil penelitian didapatkan bahwa pemberian pupuk Conway dengan dosis berbeda berpengaruh sangat nyata (P<0.01) terhadap pertumbuhan panjang mutlak dan laju pertumbuhan spesifik, serta pertumbuhan bobot mutlak E. cottonii, dengan nilai tertinggi yaitu masing-masing 1,04 cm, 0,014%, dan 0,25 g pada perlakuan dosis pupuk Conway 8 ml. Seaweed Eucheuma cottonii is one of the fishery products that have highly important economic value because it contains carrageenan which is a basic ingredient in pharmaceuticals, food, and other industrial materials. Given the large market demand for seaweed, it is necessary to make efforts to increase production. However, along with increasing demand, cultivators often experience problems, the constraints that are often experienced are, among others, the seaweed seeds produced are not optimal both in terms of quantity and quality, so there needs to be a study to produce good seaweed seed growth, namely using Conway fertilizer on seaweed culture media from tissue culture results. Conway fertilizer contains nutrients that are very beneficial for the growth of seaweed. This study aims to obtain the correct dosage of Conway fertilizer in culture media on the growth of E. cottonii seaweed seedlings. This study used E.cottoni from the Seameo Biotrop tissue culture. It used a completely randomized design with 5 treatments and 5 replications, namely the dose of Conway fertilizer (0, 2, 4, 6, and 8 ml). The stocking density of E. cottoni was 5 explants per container. The results showed that the application of Conway fertilizer with different doses had a very significant effect (P<0.01) on absolute length growth, specific growth rate, and absolute weight growth of E. cottonii, with the highest values of 1.04 cm, 0.014%, and 0,25 g, respectively, at the Conway fertilizer dose of 8 mL.

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“…The use of embryo as a medium for gene transfer is easier than the use of thallus or organ because it is a living cell resulted from callus induction and somatic embryo (Reddy et al, 2003). Gene transfer to the embryo can be implemented by using electroporator or gene pulser (Rajamuddin et al, 2010). Several promoters have been succesfully used in genetic engineering of seaweed such as cauliflower mosaic virus (CaMV), cytomegalovirus (CMV) and green fluorescent protein (GFP).…”

Section: Introductionmentioning

confidence: 99%

IN VITRO GROWTH RATE OF Kappaphycus alvarezii MICROPROPAGULE AND EMBRYO BY ENRICHMENT MEDIUM WITH SEAWEED EXTRACT

Suryati

Rosmiati

Parenrengi

et al. 2015

Indonesian Aquaculture Journal

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The development of micropropagule and embryo of seaweed depend on nutrient and fertilizer used. Seaweed has been reported contain hormone regulators such as auxine, cytokinine, gibbereline, and various minerals applied in stimulating the growth ocra plant and wheat culture. The objectives of this study were to determine the potential of Kappaphycus alvarezii extract and its optimal concentration in accelerating of Kappaphycus alvarezii micropropagule and embryo growth. Micropropagule and embryo produced through callus induction were planted into PES 1/20 liquid medium supplemented with seaweed extract at the concentrations of 0 (control), 25, 50, 75, and 100 μL in 10 mL of medium. The results showed that medium enrichment with 50 μL of seaweed extract had the highest survival rate and growth of thallus. In addition, this concentration was also resulted in a good performance of K. alvarezii thallus with the lighter color. The advantage of this study for seaweed cultivation in Indonesia, among others, seaweed can be used as fertilizer, especially in the maintenance of seaweed seed, so that cultivation can be better develop.

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KAJIAN INDUKSI KALUS RUMPUT LAUT Kappaphycus alvarezii UNTUK PRODUKSI EMBRIO SOMATIK

Cited by 6 publications

References 5 publications

Evaluation of different promoters driving the GFP reporter gene in seaweed Kappaphycus alvarezii

Evaluation of different promoters driving the GFP reporter gene in seaweed Kappaphycus alvarezii

Penggunaan Pupuk Conway Pada Media Kultur Terhadap Pertumbuhan Bibit Rumput Laut Eucheuma cottonii

IN VITRO GROWTH RATE OF Kappaphycus alvarezii MICROPROPAGULE AND EMBRYO BY ENRICHMENT MEDIUM WITH SEAWEED EXTRACT

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