Kandungan Kafein dan Karakteristik Morfologi Pucuk (Pekoe) dengan 3 Daun Muda (P+3) Enam Genotipe Teh

Martono, Budi; Udarno, Laba

doi:10.21082/jtidp.v2n2.2015.p69-76

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…Caffeine can be in the form of a white powder or shiny white needles, odorless, and has a bitter taste. Caffeine is soluble in water (1:50), alcohol (1:75), or chloroform (1:6) but less soluble in ether (Martono and Udarno, 2015).…”

Section: Resultsmentioning

confidence: 99%

The Dose Effect of Mangrove Leaf Extract (Rhizophora apiculata) on Anticancer Activity in HeLa Cells

Maulana

2021

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Disease cancer caused by abnormal growth of tissue where there has been an error, fast and out of control. Judging from the fact of gender, more than 270,000 women die every year caused by cervical cancer. To inhibit the growth of cancer cells, a compound is needed that causes the cell cycle to stop so that the ability of cell proliferation decreases. Alkaloid compounds can inhibit proliferation through oxidative inhibition processes that can cause cancer. Mangrove plants have potential as anticancer, antimicrobial, and antioxidant. The content of chemical compounds found in mangroves are flavonoids, steroids, alkaloids, phenolites, saponins and tannins. These compounds show high antioxidant activity and are shown to have a real relationship with the properties of the material's bioactivity against cancer cells. One of the mangrove species is Rhizophora apiculata. The purpose of this study was to determine the IC50 value produced by Rhizophora apiculata mangrove leaf extract on HeLa cell viability and to see the effect of Rhizophora apiculata mangrove leaf extract dosage on HeLa cell viability. The method used in this research is the experimental method. The research parameters included yield, proximate test, phytochemical test, toxicity test, total phenol test, cytotoxicity test and LC-MS test. The experimental design used was a simple and complex completely randomized design (CRD) with the Tukey test.The results of this study showed that the highest yield was in the ethanol extract of 5.91%, while the n-hexane and ethyl acetate extracts respectively had yields of 1.18% and 1.31%. The results of the proximate test on the water content of leaves and powder were 64.53% and 13.86%, respectively, the results of the ash content in the leaves and powder of Rhizophora apiculata were 3.94% and 8.41%, respectively. while the water content in the extract obtained the highest yield in the ethanol extract of 21.42%, while the n-hexane extract and ethyl acetate extract were 11.08% and 15.42%, respectively. For phytochemical results, it was found that n-hexane extract only contained alkaloids, flavonoids and steroids. Ethyl acetate extract contains steroid compounds. Meanwhile, the ethanol extract contains the most bioactive compounds, namely saponins, flavonoids, tannins and triterpenoids. The toxicity test using the Brine Shrimp Lethality Test (BSLT) method resulted in the lowest IC50 of ethanol extract at 49.45 ppm while the n-hexane and ethyl acetate extracts were 251.63 ppm and 920.45 ppm respectively. In the total phenol test, the n-hexane extract was 66.79 mg GAE / 100 gr, 222.97 mg GAE / 100 gr ethyl acetate extract and 929.04 mg GAE / 100 gr ethanol extract. HeLa cell cytotoxicity testing using the MTT method (3- (4,5-dimethiltiazol-2-yl) -2,5-dipheniltetra zolium bromide) assay resulted in the highest cell viability value at a dose of 125 ppm of 46.97%. As for the doses of 250 ppm, 500 ppm 1000 ppm, and 2000 ppm resulted in a percentage of viability of 42.95% 37.70% 35.82% and 32.12%, respectively. The IC50 value of Rhizophora apiculata leaf extract was 64.42 ppm. This value indicates that the Rhizophora apiculata extract is toxic to HeLa cells.

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Section: Resultsmentioning

confidence: 99%

The Dose Effect of Mangrove Leaf Extract (Rhizophora apiculata) on Anticancer Activity in HeLa Cells

Maulana

2021

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“…The coffee leaf tea featured 0.010 g/kg to 0.042 g/kg of oxalic acid in which much lower than that of oolong-Camelia sinensis tea. It was reported that the amount of dissolved oxalate extracted from oolong tea was 0.23 g / kg was [29].…”

Section: Characteristics Of Brewed Coffee Leaf Teamentioning

confidence: 99%

Consumer perception of decocted coffee leaf tea originated from different altitude

Fibrianto,

Sholihah,

Bimo

2024

IOP Conf. Ser.: Earth Environ. Sci.

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It is commonly known that plantation location influences the composition of the coffee component as well as the leaves. Therefore, it was expected that the difference location will also affect the quality of decocted one. In this current study, effect of plantation location is investigated on Arabica and Robusta coffee leaf tea. Two different coffee plantations were chosen as the region of observation (560 ASL and 1700 ASL). All the coffee leaf tea was brewed by decoction method, in which sensorially optimized by Just about Right (JAR) method. The sensory attributes of concern were included sweet, sour, bitter taste and astringent mouth-feel. Considering consumer preference on the brewed coffee leaf tea, the decoction brewing condition were set on 95°C for 5 minutes. Additionally, coffee leaf tea grown at higher altitudes exhibits significantly greater total phenolic content (TPC) and antioxidant activity (p < 0.05). Not only does this tea have higher TPC and antioxidant activity at higher altitudes, but it also shows superior consumer sensory acceptability. This may be supported by the fact that higher altitude coffee leaf tea has more diverse sensory driver components than that of lower altitude coffee leaf tea as suggested by LC-MS.

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Effect of fixation methods and various clones of Camellia sinensis var. sinensis (L) properties and antioxidant activity of Indonesian green tea

ULA¹,

Prawira-Atmaja

Maulana³

et al. 2022

International Journal of Secondary Metabolite

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Fixation is essential in green tea processing to inactivate the polyphenol oxidase enzyme. In Indonesia, green tea is made from the Assam variety and produced using the panning method. Few studies are reported on green tea made from Indonesian clones of the Sinensis variety. This study aims to identify chemical characteristics, antioxidant activity, and sensory evaluation of green tea from local clones of the Sinensis variety (GMBS 2, GMBS 4, and GMBS 5) with different fixation methods (panning and steaming). The results show that the caffeine content of green tea products ranged from 2.51-2.59% and 2.67-2.74% for panning and steaming methods. The panning method produced green tea with higher total polyphenol and flavonoid content than the steaming method. Green tea with the panning method has an IC50 value of 14.45; 14.41; and 17.41 mg/L for GMBS 2, GMBS 4, and GMBS 5, respectively. The panning method resulted in a smaller IC50 value than the steaming method for GMBS 2 and GMBS 4 clones. The steaming method produced green tea with a higher taste, aroma, and total score than those the panning method. However, different fixation methods did not significantly affect the appearance, liquor color, and leaf infusion. In conclusion, different fixation methods on GMBS 2, GMB 4, and GMB 5 produced green tea products that met the Indonesian National Standard 3945:2016. Further research is needed to determine the role of the plucking period/season and the characteristics of volatile compounds of green tea from GMBS clones with different fixation methods.

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Kandungan Kafein dan Karakteristik Morfologi Pucuk (Pekoe) dengan 3 Daun Muda (P+3) Enam Genotipe Teh

Cited by 3 publications

References 11 publications

The Dose Effect of Mangrove Leaf Extract (Rhizophora apiculata) on Anticancer Activity in HeLa Cells

The Dose Effect of Mangrove Leaf Extract (Rhizophora apiculata) on Anticancer Activity in HeLa Cells

Consumer perception of decocted coffee leaf tea originated from different altitude

Effect of fixation methods and various clones of Camellia sinensis var. sinensis (L) properties and antioxidant activity of Indonesian green tea

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