KARAKTERISASI DAN SKRINING FITOKIMIA SERTA ANALISIS FLAVONOID DARI BUAH MENGKUDU (Morindacitrifolia L) SECARA KROMATOGRAFI LAPIS TIPIS

Abstract

Noni fruit (Morindacitrifolia L.) is a medicinal plant that is well known by the people in Indonesia. Noni is efficacious as an immune enhancer, normalizes blood pressure, anti-cancer, analgesic, diabetes mellitus, heart disease, stroke, antimicrobial. To know the characteristics of simplicia and the class of compounds from the noni fruit as well as to analyze the flavonoid compounds contained in the noni fruit using the thin layer chromatography method. The flavonoid compounds were extracted by maceration using ethanol solvent and then the ethanol extract was tested by thin-layer chromatography using 1% HCl mobile phase and spotting with 1 N H2SO4 reagent and methanol pa (1: 1% v/v). Result of simplicia characterization of fruit Noni, obtained 1.32% water content, 38.41 % water soluble extract content, 16.7% ethanol soluble extract content, 6.09% total ash content, and 0.45 acid insoluble ash content. %. Noni fruit simplicia contains chemical compounds of alkaloids, tannins, flavonoids, triterpenoids/steroids, anthraquinones, saponins with phytochemical screening tests. The value of Rf from the analysis of noni fruit flavonoid compounds using the Thin Layer Chromatography method with 3 repetitions is 0.75; 0.68 and 0.62. This indicates the presence of flavonoid compounds in the noni fruit.

PROFIL SENYAWA FLAVONOID EKSTRAK BUAH MENGKUDU (Morinda citrifolia L.) DENGAN BERBAGAI METODE PENGERINGAN

“…Buah, biji, kulit, daun, dan bunga tanaman mengkudu mengandung mineral seperti kalium, zat besi, garam, dan kalsium serta vitamin A, C, tiamin, niasin, dan riboflavin (Abou Assi et al, 2017;Garnida & Hasnelly, 2018). Oleh sebab itu, buah mengkudu dikenal memiliki banyak manfaat kesehatan bagi manusia, termasuk adanya berbagai bahan kimia yang mencakup sebagai antibakteri (Juariah et al, 2021), analgesik, hipotensi (Nagalingam et al, 2013), antivirus (Khoirunnisa & Sumiwi, 2019), antijamur, antitumor (Abou Assi et al, 2017), anti inflamasi dan antibiotik (Kaleem & Ahmad, 2018) serta sebagai pengobatan tradisional obat AIDS, radang sendi, batuk, diare, diabetes (Ali et al, 2016) dan antihipertensi (Sitorus et al, 2021).…”

“…Buah mengkudu mengandung beberapa senyawa seperti flavonoid, alkaloid, saponin, antrakuinon dan triterpenoid (Mudaliana et al, 2019). Sedangkan, hasil uji skrining fitokimia pada ekstrak buah mengkudu mengandung senyawa kimia alkaloid, tanin, flavonoid, triterpenoid/steroid, antrakuinon, saponin (Sitorus et al, 2021). Dibandingkan dengan jus atau buah mengkudu segar, buah mengkudu fermentasi belum terbukti lebih baik, karena hasil fermentasi buah mengkudu hanya mengandung flavonoid dan triterpenoid serta masih terdapat kapang/khamir yang akan tumbuh (Mudaliana et al, 2019).…”