Karyotype variability in KP1(+) and KP1(−) strains of Trypanosoma rangeli isolated in Brazil and Colombia

Cabrine-Santos, Marlene; Ferreira, Keila Adriana Magalhães; Tosi, Luiz Ricardo Orsini; Lages‐Silva, Eliane; Ramı́rez, Luı́s Eduardo; Pedrosa, André Luiz

doi:10.1016/j.actatropica.2009.01.004

Cited by 12 publications

(11 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…T. rangeli presents an extensive genetic variability demonstrated by kDNA and nuclear DNA analysis (7,9,11,12). Moreover, analysis of the karyotype profiles permitted the division of the T. rangeli strains into two groups coinciding with the KP1+ and KP1− genotypes (13). Subsequent studies clearly showed host-parasite specific associations between T. rangeli-Rhodnius spp.…”

mentioning

confidence: 98%

Susceptibilidad de diferentes especies de Rhodnius (Hemiptera, Reduviidae, Triatominae) a una cepa brasileña de Trypanosoma rangeli (SC58/KP1-)

et al. 2014

View full text Add to dashboard Cite

Introduction: Specific host-parasite associations have been detected experimentally and suggest that triatomines of the genus Rhodnius act as biological filters in the transmission of Trypanosoma rangeli. Objective: To analyze the susceptibility of four Rhodnius species (Rhodnius robustus, Rhodnius neglectus, Rhodnius nasutus and Rhodnius pictipes) to a Brazilian strain of T. rangeli (SC-58/KP1-). Materials and methods:We selected thirty nymphs of each species, which were fed on blood infected with T. rangeli. Periodically, samples of feces and hemolymph were analyzed. Triatomines with T. rangeli in their hemolymph were fed on mice to check for transmission by bites. Later, the triatomines were dissected to confirm salivary gland infection. Results: Specimens of R. pictipes showed higher rates of intestinal infection compared to the other three species. Epimastigotes and trypomastigotes were detected in hemolymph of four species; however, parasitism was lower in the species of the R. robustus lineage. Rhodnius robustus and R. neglectus specimens did not transmit T. rangeli by bite; after dissection, their glands were not infected. Only one specimen of R. nasutus and two of R. pictipes transmitted the parasite by bite. The rate of salivary gland infection was 16% for R. pictipes and 4% for R. nasutus. Conclusions: Both infectivity (intestinal, hemolymphatic and glandular) and transmission of T. rangeli (SC58/KP1-) were greater and more efficient in R. pictipes. These results reinforce the hypothesis that these triatomines may act as biological filters in the transmission of T. rangeli.

show abstract

mentioning

confidence: 98%

Susceptibilidad de diferentes especies de Rhodnius (Hemiptera, Reduviidae, Triatominae) a una cepa brasileña de Trypanosoma rangeli (SC58/KP1-)

et al. 2014

View full text Add to dashboard Cite

show abstract

“…In addition, the subtelomeric region of T. rangeli contains genes of the gp85/TS superfamily that presents sialidase activity (Añez-Rojas et al 2005) and might be involved in attachment of the parasite to the salivary glands of the vector (Peña et al 2009). Furthermore, analysis of the telomeric and subtelomeric regions of T. rangeli may contribute to the elucidation of the mechanisms that are responsible for the karyotype variability observed in this parasite (Henriksson et al 1996;Cabrine-Santos et al 2009). …”

Section: Introductionmentioning

confidence: 99%

“…Different molecular methods have been used for the genetic characterization of T. rangeli (Vallejo et al 2002;Marquez et al 2007;Cabrine-Santos et al 2009). T. rangeli populations can be divided into two groups, called KP1(+) and KP1(−), according to the presence or absence of KP1 minicircles in their kDNA, respectively (Vallejo et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Sequencing and analysis of chromosomal extremities of Trypanosoma rangeli in comparison with Trypanosoma cruzi lineages

et al. 2010

Self Cite

View full text Add to dashboard Cite

The aim of this study was to investigate the genetic variability of sequences present in the chromosome ends of Trypanosoma rangeli strains defined by the presence (+) or absence (-) of KP1 minicircles, and to compare the mean terminal restriction fragment (TRF) lengths to those of Trypanosoma cruzi populations representative of groups TcI, TcII, TcIV, and TcVI. Southern blots containing RsaI-digested genomic DNA of T. rangeli KP1(+) strains, T. rangeli KP1(-) strains, and T. cruzi strains were probed with the previously described subtelomeric sequences (170 bp) of T. rangeli and with telomeric hexamer repeats. Mean TRF length analysis showed that the chromosome ends of T. rangeli are distinctly organized, with TRFs ranging from 1.3 to 9 kb for KP1(+) strains and from 0.3 to 5.0 kb for KP1(-) strains. In T. cruzi, TRF length ranged from 0.2 to 9 kb and no association with the genotype of the parasite could be established. Sequence analysis of the 170-bp amplicons revealed the occurrence of sequence polymorphisms in the subtelomeric region between and within KP1(+) and KP1(-) strains. The GTT triplet was detected in all KP1(+) strains, except for strain Cas4, but not in any of the KP1(-) strains. The dendrogram constructed by alignment of all T. rangeli strains showed the division into two main groups, mainly related to the presence or absence of the KP1 minicircle. In conclusion, the present results extend the genotype differences demonstrated by kDNA and karyotype analysis in T. rangeli to the chromosome ends of the parasite.

show abstract

“…PFGE was performed in a BioRad CHEF Mapper system, as previously described (7) with pulses of 6V/cm and 46.47s for 33h 36 min. Analysis of chromosomal bands shared between clonal lineages and parental strains was conducted with the GelCompar II Program (Applied Maths, Kortrijk, Belgium) with the following conditions: Dice (Opt.1.00%) (Tol 1.0%-1.0%) (H>0.0% S>0.0%)[0.0%-100.0%].…”

mentioning

confidence: 99%

“…Epimastigote forms of T. cruzi (strains Y, JG, and RN1) and T. rangeli (strains P07 and SO29) (7) were maintained by passaging in LIT medium (4) supplemented with 10% (v/v) fetal bovine serum. These were incubated at 28ºC in a biochemical oxygen demand (BOD) incubator.…”

mentioning

confidence: 99%

Semisolid liver infusion tryptose supplemented with human urine allows growth and isolation of Trypanosoma cruzi and Trypanosoma rangeli clonal lineages

Fajardo

Cabrine-Santos

Ferreira

et al. 2016

Rev. Soc. Bras. Med. Trop.

Self Cite

View full text Add to dashboard Cite

Introduction: This work shows that 3% (v/v) human urine (HU) in semisolid Liver Infusion Tryptose (SSL) medium favors the growth of Trypanosoma cruzi and T. rangeli. Methods: Parasites were plated as individual or mixed strains on SSL medium and on SSL medium with 3% human urine (SSL-HU). Isolate DNA was analyzed using polymerase chain reaction (PCR) and pulsed-field gel electrophoresis (PFGE). Results: SSL-HU medium improved clone isolation. PCR revealed that T. cruzi strains predominate on mixed-strain plates. PFGE confirmed that isolated parasites share the same molecular karyotype as parental cell lines. Conclusions: SSL-HU medium constitutes a novel tool for obtaining T. cruzi and T. rangeli clonal lineages.

show abstract

Karyotype variability in KP1(+) and KP1(−) strains of Trypanosoma rangeli isolated in Brazil and Colombia

Cited by 12 publications

References 38 publications

Susceptibilidad de diferentes especies de Rhodnius (Hemiptera, Reduviidae, Triatominae) a una cepa brasileña de Trypanosoma rangeli (SC58/KP1-)

Susceptibilidad de diferentes especies de Rhodnius (Hemiptera, Reduviidae, Triatominae) a una cepa brasileña de Trypanosoma rangeli (SC58/KP1-)

Sequencing and analysis of chromosomal extremities of Trypanosoma rangeli in comparison with Trypanosoma cruzi lineages

Semisolid liver infusion tryptose supplemented with human urine allows growth and isolation of Trypanosoma cruzi and Trypanosoma rangeli clonal lineages

Contact Info

Product

Resources

About