Karyotypes, C-banding, and chromosomal location of active nucleolar organizing regions in Tapinoma (Hymenoptera, Formicidae)

Palomeque, Teresa; Chica, Eduardo J.; Cano, M. A.; Guardia, R. Díaz de la

doi:10.1139/g88-047

Cited by 21 publications

(20 citation statements)

References 13 publications

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“…In T. chanchamayoensis, a marked reaction was seen with DA/CMA 3 ( Figure 4A), while in T. recursa, the staining with CMA 3 revealed no GC-rich regions in the chromosomes ( Figure 4B). The positive reactions seen were probably associated with nucleolus organizing regions (NORs), as observed in 188 Costa et al other insects such as Orthoptera (Camacho et al, 1991), Coleoptera (Maffei et al, 2001), and in other Hymenoptera, including Formicidae (Palomeque et al, 1988;Imai et al, 1992), Sphecidae (Araújo et al, 2000) and Meliponini (Brito et al, 1999(Brito et al, , 2003Caixeiro, 1999;Rocha, 2000). Some authors have suggested that the number of NORs revealed by impregnation with silver nitrate or by CMA 3 staining is an important taxonomic character.…”

Section: Resultsmentioning

confidence: 73%

Karyotypic description of four species of Trigona (Jurine, 1807) (Hymenoptera, Apidae, Meliponini) from the State of Mato Grosso, Brazil

2004

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The genus Trigona contains at least 31 species, but there have been few cytogenetic studies of this group. In this work, four species of Trigona (T. branneri, T. chanchamayoensis, T. hyalinata, and T. recursa) from the municipality of Cuiabá, in the State of Mato Grosso, Brazil, were studied. In all of the species, the females had 2n = 34 chromosomes and the males had n = 17. The C-banding patterns showed that the karyotypes of these species consisted mainly of acrocentric and pseudoacrocentric chromosomes. These cytogenetic findings should useful in future phylogenetic studies of this group.

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Section: Resultsmentioning

confidence: 73%

Karyotypic description of four species of Trigona (Jurine, 1807) (Hymenoptera, Apidae, Meliponini) from the State of Mato Grosso, Brazil

2004

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“…NORs adjacent to C bands have also been observed in some insects (Palomeque et al, 1988;Rodríguez-Iñigo et al, 1992;Lorite et al, 1997;Souza et al, 1998). The mitotic metaphases of N. cruentata submitted to the base-specific fluorochromes DAPI/ MM and analyzed with DAPI filter have evidenced that the negative heteropycnotic regions in the chromosomic pairs 1, 2 and 3 do not posses highly repeated AT base sequences, since these regions are DAPI negative.…”

Section: Discussionmentioning

confidence: 93%

Cytogenetic analysis of the neotropical spider Nephilengys cruentata (Araneomorphae, tetragnathidae): standard staining, NORs, C-bands and base-specific fluorochromes

2005

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The aim of this work is to characterize Nephilengys cruentata in relation to the diploid number, chromosome morphology, type of sex determination chromosome system, chromosomes bearing the Nucleolar Organizer Regions (NORs), C-banding pattern, and AT or GC repetitive sequences. The chromosome preparations were submitted to standard staining (Giemsa), NOR silver impregnation, C-banding technique, and base-specific fluorochrome staining. The analysis of the cells showed 2n = 24 and 2n = 26 chromosomes in the embryos, and 2n = 26 in the ovarian cells, being all the chromosomes acrocentric. The long arm of the pairs 1, 2 and 3 showed an extensive negative heteropycnotic area when the mitotic metaphases were stained with Giemsa. The sexual chromosomes did not show differential characteristics that allowed to distinguish them from the other chromosomes of the complement. Considering the diploid numbers found in N. cruentata and the prevalence of X 1 X 2 sex determination chromosome system in Tetragnathidae, N. cruentata seems to possess 2n = 24 = 22 + X 1 X 2 in the males, and 2n = 26 = 22 + X 1 X 1 X 2 X 2 in the females. The pairs 1, 2 and 3 showed NORs which are coincident with the negative heteropycnotic patterns. Using the C-banding technique, the pericentromeric region of the chromosomes revealed small quantity or even absence of constitutive heterochromatin, differing of the C-banding pattern described in other species of spiders. In N. cruentata the fluorochromes DAPI/ DA, DAPI/MM and CMA 3 /DA revealed that the constitutive heterochromatin is rich in AT bases and the NORs possess repetitive sequences of GC bases.Key words: chromosome, Araneae, heterochromatin, secondary constriction, Chromomycin A 3 . RESUMOAnálise citogenética da aranha neotropical Nephilengys cruentata (Araneomorphae, Tetragnathidae): coloração convencional, RONs, bandas C e fluorocromos base-específicos O objetivo deste trabalho é caracterizar Nephilengys cruentata em relação ao número diplóide, à morfologia cromossômica, ao tipo de sistema cromossômico de determinação sexual, aos cromossomos portadores de Regiões Organizadoras de Nucléolo (RONs), padrão de bandas C e seqüências AT ou GC repetitivas. As preparações cromossômicas foram submetidas à coloração convencional (Giemsa), à impregnação pelo nitrato de prata, técnica de obtenção de bandas C e à coloração com fluorocromos base-específicos. A análise das células mostrou 2n = 24 e 2n = 26 cromossomos nos embriões e 2n = 26 nas células ovarianas, sendo todos cromossomos acrocêntricos. O braço longo dos pares 1, 2 e 3 apresentou extensa região heteropicnótica negativa quando as metáfases mitóticas foram coradas com Giemsa. Os cromossomos sexuais Braz. J. Biol., 65(2): 193-202, 2005 194 ARAÚJO, D., CELLA, D. M. and BRESCOVIT, A. D.species possess the type X 1 X 2 X 3 X 4 /X 1 X 1 X 2 X 2 X 3 X 3 X 4 X 4 (Datta & Chatterjee, 1983), 5 species possess the type X 1 X 2 X 3 Y/X 1 X 1 X 2 X 2 X 3 X 3 (Maddison, 1982) and 2 species show the type X 1 X 2 Y/X 1 X 1 X 2 X 2 (Silva, 1988...

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“…In a previous article (Palomeque et al, 1988) we have reported the existence in Tapinoma nigerrimum of an Ag-positive region (or Ag-NOR) in chromosomes 6 and 8. Amongst the remaining chromosomes, the number of chromosomes bearing an Ag-positive region varied between individuals and populations.…”

Section: Introductionmentioning

confidence: 96%

Analysis of the nucleolar organizing regions in the ant Tapinoma nigerrimum (Hymenoptera, Formicidae)

et al. 1997

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This study analyses the NORs of Tapinoma nigerrimum, a species that, as known from previous studies, has various chromosomes which carry a NOR site. The analysis was made by a combination of three methods: silver nitrate staining, in situ hybridization with fluorescein-or digoxigenin-labelled probes, and staining with the CG-specific fluorochrome chromomycin A3. The silver staining technique showed an Ag-positive region on chromosome 6 and on various other chromosomes. However, the application of in situ hybridization techniques showed only one positive signal in the proximal region of the short arm of chromosome 6 of T nigerrimum. Similar results were observed by CMA banding. The absence of rDNA genes or the presence of only a small number of these, not detectable with the above probes, might explain the absence of hybridization signal in the remaining chromosomes.

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Karyotypes, C-banding, and chromosomal location of active nucleolar organizing regions in Tapinoma (Hymenoptera, Formicidae)

Cited by 21 publications

References 13 publications

Karyotypic description of four species of Trigona (Jurine, 1807) (Hymenoptera, Apidae, Meliponini) from the State of Mato Grosso, Brazil

Karyotypic description of four species of Trigona (Jurine, 1807) (Hymenoptera, Apidae, Meliponini) from the State of Mato Grosso, Brazil

Cytogenetic analysis of the neotropical spider Nephilengys cruentata (Araneomorphae, tetragnathidae): standard staining, NORs, C-bands and base-specific fluorochromes

Analysis of the nucleolar organizing regions in the ant Tapinoma nigerrimum (Hymenoptera, Formicidae)

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