Karyotypic analysis of the human monoblastic cell line U937

Shipley, Janet; Sheppard, David M.; Sheer, Denise

doi:10.1016/0165-4608(88)90195-1

Cited by 19 publications

(39 citation statements)

References 24 publications

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“…A critical unanswered question is how and to what extent the CALMIAF1O fusion contributes to the malignant phenotype of U937. The marker 10 chromosome described by Shipley and coworkers (52), which represents the der (10), is present in all sublines of U937 examined. The fact that this translocation occurs in a cell line that is used frequently in various biological analyses enhances its importance.…”

Section: Discussionmentioning

confidence: 99%

The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.

Dreyling

Martínez-Climent

Zheng

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

287

290

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The translocation t(10;11)(p13;q14) is a recurring chromosomal abnormality that has been observed in patients with acute lymphoblastic leukemia as well as acute myeloid leukemia. We have recently reported that the monocytic cell line U937 has a t(10;11)(pl3;ql4) translocation. Using a combination of positional cloning and candidate gene approach, we cloned the breakpoint and were able to show that AF1O is fused to a novel gene that we named CALM (Clathrin Assembly Lymphoid Myeloid leukemia gene) located at 11ql4. AFIO, a putative transcription factor, had recently been cloned as one of the fusion partners of MLL. CALM has a very high homology in its N-terminal third to the murine ap-3 gene which is one of the clathrin assembly proteins. The N-terminal region of ap-3 has been shown to bind to clathrin and to have a high-affinity binding site for phosphoinositols. The identification of the CALM/AFIO fusion gene in the widely used U937 cell line will contribute to our understanding of the malignant phenotype of this line.

show abstract

Section: Discussionmentioning

confidence: 99%

The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.

Dreyling

Martínez-Climent

Zheng

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

287

290

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“…Conventional banding data of U937 showed many of the same marker chromosomes previously described (Shipley et al, 1988). Karyotypic analysis with conventional banding techniques often leaves complex derivative chromosomes unclassified.…”

Section: Discussionmentioning

confidence: 95%

“…The marker chromosomes reported by Shipley et al (1989) were identified using G-banded analysis from three individual sources. The consistent aberrations detected in all three cell lines, were 3p-, 11q-, 16p+ and 17p- (Shipley et al, 1988). The ISCN nomenclature (ISCN 1995) for the G-banded karyotype from this present study is shown in Table 2. U937 exhibited a hyperdiploid chromosome complement (51-57 chromosomes per cell).…”

Section: G-banded and M-fish Analysismentioning

confidence: 89%

“…G-banded analysis revealed several rearrangements previously described (Shipley et al, 1988). The marker chromosomes reported by Shipley et al (1989) were identified using G-banded analysis from three individual sources.…”

Section: G-banded and M-fish Analysismentioning

confidence: 99%

“…Apart from the PICALM: MLLT10 gene fusion, very little is known about the genetic abnormalities of U937. Its karyotype is complex (Shipley et al, 1988) and the true identities of many rearrangements have not yet been resolved. Although the t(10;11) and its genetic role in leukaemogenesis has been well characterised (Dreyling et al, 1996), little is known about other significant genetic events in this cell line and their potential synergistic effects.…”

mentioning

confidence: 99%

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The characterisation of the lymphoma cell line U937, using comparative genomic hybridisation and multi-plex FISH

Strefford¹,

Foot²,

Chaplin³

et al. 2001

Cytogenet Genome Res

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The cell line U937, which has been used extensively for studies of myeloid differentiation, bears the t(10;11)(p13;q14) translocation which results in a fusion between the MLLT10 (myeloid/lymphoid or mixed-lineage leukemia [trithorax, Drosophila, homolog]; translocated to 10; alias AF10) gene and the Ap-3-like clathrin assembly protein, PICALM (Clathrin assembly lymphoid myeloid leukaemia). Apart from this translocation, very little is known about the other genetic alterations in this cell line that may represent significant events in disease progression. In this study, conventional G-banding, CGH and M-FISH have been used to characterise fully all of the cytogenetic alterations present in the U937 cell line. M-FISH analysis confirmed the presence of the t(10;11) and an apparently normal copy of both chromosomes 10 and 11. A t(1;5) translocation was observed as well as several unbalanced rearrangements. CGH detected amplifications resulting from duplications of 2q, 6p and 13q. These changes could result in fusion gene products involved in carcinogenesis or the positions of putative oncogenes and tumour suppressor genes. A good correlation between conventional G-banding, CGH and M-FISH was observed.

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Widespread intraspecies cross-contamination of human tumor cell lines arising at source

et al. 1999

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Karyotypic analysis of the human monoblastic cell line U937

Cited by 19 publications

References 24 publications

The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.

The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.

The characterisation of the lymphoma cell line U937, using comparative genomic hybridisation and multi-plex FISH

Widespread intraspecies cross-contamination of human tumor cell lines arising at source

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