Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis

Nakabayashi, Ryo; Hashimoto, Kei; Toyooka, Kiminori; Saito, Kazuki

doi:10.1007/s11306-019-1486-5

Cited by 30 publications

(38 citation statements)

References 24 publications

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“…One advantage of the presented approach was the use of copper foil tape to adhere the cryofilm to the target slide. Previous studies have utilised double‐sided carbon tape, such as that used in the mounting of EM tissue sections, while a recent study in plants has also reported the use of copper foil tape to adhere tape‐transferred sections to MALDI targets . The carbon tape surface is not flat and contains frequent pits of varying sizes that can create variable ion signal in a MALDI mass spectrometer due to the fixed nature of the laser focus.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, stabilisation of the tissue section prior to cutting, by using a tape transfer system has been useful for the collection of fresh frozen sections from some tissue types. Recently, methods for collection and MALDI‐IMS analysis of plant tissue sections using tape transfer were reported. Importantly, while the transfer tape is nonconductive, analysis of small molecules and metabolites was possible using MALDI‐FT‐ICR mass spectrometry, for which the use of a conductive target plate is not required.…”

Section: Introductionmentioning

confidence: 99%

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Detection of small molecule concentration gradients in ocular tissues and humours

et al. 2019

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The eye is an elegant organ consisting of a number of tissues and fluids with specialised functions that together allow it to effectively transmit and transduce light input to the brain for visual perception. One key determinant of this integrated function is the spatial relationship of ocular tissues. Biomolecular distributions within the main ocular tissues cornea, lens, and retina have been studied extensively in isolation, yet the potential for metabolic communication between ocular tissues via the ocular humours has been difficult to visualise. To address this limitation, the current study presents a method to map spatial distributions of metabolites and small molecules in whole eyes, including ocular humours. Using a tape-transfer system and freeze-drying, the spatial distribution of ocular small molecules was investigated in mouse, rat, fish (black bream), and rabbit eyes using negative ion mode MALDI imaging mass spectrometry. Full-scan imaging was used for discovery experiments, while MS/MS imaging for identification and localisation was also demonstrated. In all eyes, metabolites such as glutathione and phospholipids were localised in the main ocular tissues. In addition, in rodent eyes, major metabolites were distributed relatively uniformly in ocular humours.In contrast, both uniform and spatially defined ocular metabolite distributions were observed in the black bream eye. Tissue and ocular humour distributions were reproducible, as demonstrated by the three-dimensional analysis of a mouse eye, and able to be captured with high spatial resolution analysis. The presented method could be used to further investigate the role of inter-tissue metabolism in ocular health, and to support the development of therapeutics to treat major ocular diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of small molecule concentration gradients in ocular tissues and humours

et al. 2019

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“…Advances in MS imaging (MSI) techniques (Mhlongo et al, 2018) have made it possible to map metabolites in intact plant tissues at sub-cellular level and connect the spatial complexity of the plant molecular organization to phenotypical features. Some techniques that have been hyphenated with MSI for imaging metabolites in plant specimens are matrix-assisted laser desorption/ionization (MALDI) and laser ablation electrospray ionization (LAESI) (Etalo et al, 2015;Nakabayashi et al, 2019). However, these approaches have not yet been utilized in ENMplant interaction studies.…”

Section: Data Acquisitionmentioning

confidence: 99%

Omics to address the opportunities and challenges of nanotechnology in agriculture

Majumdar

Keller

2020

Critical Reviews in Environmental Science and Technology

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Increasing global food demand, and risks associated with climate change and agrochemicals demand novel and sustainable agricultural practices to improve crop yield and quality. Various strategies using nanotechnology have been explored widely to provide solutions. However, their application in the food industry is constrained by limited understanding of nanomaterial safety. Growing interest in the potential of engineered nanomaterials (ENMs) in agricultural applications has also resulted in increased crop exposure, leading to unknown risks to plants, animals and humans. Recently, considerable research has been carried out on overt plant response to ENMs; however, conclusive mechanistic information is lacking. With advancements in hyphenated analytical techniques, research on ENM-biota interactions has witnessed a paradigm shift from low-throughput, single end-point bioassays to high-throughput, discovery-oriented omic tools, including transcriptomics, proteomics and metabolomics. This review summarizes the pioneering studies utilizing omics in plants exposed to ENMs to explain phenotypic expressions and elucidate associated biological pathways. Advantages and challenges of current analytical techniques employed in proteomics and metabolomics are also discussed. We acknowledge the need for integration and application of multiomics to identify sensitive biomarkers in plants in response to ENMs, and provide mechanistic insights, in order to design enhanced and safer nano-enabled products for agriculture.

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“…There is also a method to transfer only the tissue from the film to the glass slide using an ultraviolet light source, but this requires a very advanced technique and specialized equipment [58]. Recently, Nakabayashi et al [59] reported a technique to directly transfer a prepared section from a film to ITO-coated glass slides, which improves sample preparation for plant tissues with a high water content.…”

Section: Sectioningmentioning

confidence: 99%

Application of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging for Food Analysis

Morisasa

Satō

Kimura

et al. 2019

Foods

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Food contains various compounds, and there are many methods available to analyze each of these components. However, the large amounts of low-molecular-weight metabolites in food, such as amino acids, organic acids, vitamins, lipids, and toxins, make it difficult to analyze the spatial distribution of these molecules. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging is a two-dimensional ionization technology that allows the detection of small metabolites in tissue sections without requiring purification, extraction, separation, or labeling. The application of MALDI-MS imaging in food analysis improves the visualization of these compounds to identify not only the nutritional content but also the geographical origin of the food. In this review, we provide an overview of some recent applications of MALDI-MS imaging, demonstrating the advantages and prospects of this technology compared to conventional approaches. Further development and enhancement of MALDI-MS imaging is expected to offer great benefits to consumers, researchers, and food producers with respect to breeding improvement, traceability, the development of value-added foods, and improved safety assessments.

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Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis

Cited by 30 publications

References 24 publications

Detection of small molecule concentration gradients in ocular tissues and humours

Detection of small molecule concentration gradients in ocular tissues and humours

Omics to address the opportunities and challenges of nanotechnology in agriculture

Application of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging for Food Analysis

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