Kemampuan Blastospora Paecilomyces fumosoroseus, Metarhizium anisopliae dan Lecanicillium decanii dalam Menginfeksi Kumbang Hypothenemus hampei

Abstract

<p><em>Hypothenemus hampei is a coffee </em><em>berry</em><em> borer</em><em>s</em><em> (C</em><em>B</em><em>B) </em><em>that</em><em> is difficult to control because it lives inside the coffee </em><em>berries</em><em>. </em><em>B</em><em>lastospor</em><em>es of </em><em>entomopathogen fung</em><em>al</em><em> are expected to be effective in controlling these pests. The </em><em>purpose </em><em>of this study was to </em><em>evaluate</em><em> the ability of blastospores Paecilomyces fumosoroseus, Metarhizium anisopliae and Lecanicillium lecanii to infect, kill and inhibit the development of H. hampei on coffee cherries in the laboratory. The study was conducted from March to December 2019 at the Plant Protection Laboratory, </em><em>Indonesian </em><em>Industrial </em><em>and Beverage Crops Research Institute</em><em>, Sukabumi.</em><em> </em><em>The study used 14 treatments, namely 3 types of fungi are P. fumosoroseus, M. anisopliae and L. lecanii with every 4 densities are 10<sup>8</sup>, 10<sup>7</sup>, 10<sup>6</sup>, and 10<sup>5</sup> blastospores/ml, positive control using botanical insecticides (Biotris) and negative control, and repeated 4 times.</em><em> The parameters observed were mortalit</em><em>ies</em><em>, number of </em><em>bor holes,</em><em> and insect development after treatment. The results showed that all blastospores were able to infect and kill H. imago in the laboratory. </em><em>The highest mortality occurred in the treatment of P. fumosoroseus with 10<sup>8</sup> blastospores/ml densities (P1) by 39.91%. </em><em>B</em><em>lastospores </em><em>of 3 entomopathogens </em><em>were able to inhibit the </em><em>number</em><em> of</em><em> bor holes</em><em> on the coffee </em><em>cherries</em><em>, with the highest inhibition in the treatment of L. lecanii 10<sup>6</sup> blastospores/ml</em><em> (L3)</em><em> by 28.57%. All types of blastospores can also inhibit insect development in the coffee cherries. Treatment of M. anisopliae </em><em>10<sup>8</sup> blastospore/ml </em><em>(M1) was able to reduce the number of eggs and nymph</em><em>s by </em><em>78.69% and 74.24% respectively compared to negative controls. Blastospores from three types of entomopathogens have the potential to be developed as bioinsecticides </em><em>to</em><em> control C</em><em>B</em><em>B.</em></p>