KEMAMPUAN Trichoderma sp. DAN FILTRATNYA DALAM MENEKAN PERTUMBUHAN Sclerotium rolfsii SECARA IN VITRO

Indigenous fungi can be found around bamboo clumps. This study aimed to identify indigenous fungi isolated from the bamboo clump as biological control agents against pathogenic fungi of Fusarium sp. in garlic. The study was conducted from June to September 2021 at the Laboratory of Pest and Disease Observation, Temanggung Regency, Central Java, Indonesia. The current research design involved the following three steps, (1) Isolation and identification of the pathogenic fungi of Fusarium sp. in garlic, (2) Isolation and identification of indigenous fungi collected from the bamboo clump, and (3) Antagonism test of indigenous fungi isolated from the bamboo clump against Fusarium sp. in-vitro. The results showed that ten species were successfully identified, and five species had the potential as biological control agents against Fusarium sp. in garlic; Penicillium sp., Mucor sp., Aspergillus sp1, Aspergillus sp2, and Tricho-derma sp. Trichoderma sp. revealed the highest antagonism (66.71%), while Penicillium sp. revealed the lowest antagonism (32.925%) against Fusarium sp. Based on their antagonistic potential, Trichoderma sp. showed the highest ability to suppress Fusarium sp. (66.71%), while the lowest one was Penicillium sp. (32.92%).

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“…According to Dewi et al (2015), the growth of Trichoderma sp. tended to be faster, suppressing pathogenic fungi growth.…”

Section: Discussionmentioning

confidence: 99%

Antagonism of Indigenous Fungi Collected from the Bamboo Clump against Fusarium sp., the Cause of Fusarium Wilt Disease in Garlic

Lestiyani

Fauziah²,

Sugiyarto³

2022

J. Proteksi. Tanaman

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“…Berdasarkan hasil uji daya hambat seperti pada Tabel 1. memperlihatkan bahwa masing-masing isolat memiliki daya hambatan yang bervariasi dan secara statistik berbeda nyata persentase penghambatan berkisar dari 7,31% -23,93%. Persentase penghambatan terbesar terdapat pada isolat Trichoderma sp yang diisolasi dari Sinar Baru, Rantau Badauh, Kalimantan Selatan dengan persentase penghambatan sebesar 23,93% dan persentase penghambatan terkecil diperlihatkan pada (Dewi, 2015).…”

Section: Isolasi Colletotrichum Gleosporioidesunclassified

Pengujian Dua Belas Jenis Trichoderma Untuk Mengendalikan Penyakit Antraknosa (Colletotrichum gloeosporioides) pada Bawang Merah (Allium ascalonicum L.)

Diastari

Salamiah

Soedijo

2021

JPTT

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Telah dilakukan penelitian uji dua belas jenis Trichoderma untuk mengendalikan penyakit antraknosaColletotrichum gloeosporioides pada pertanaman bawang merah. di Kalimantan Selatan tujuandaripada penelitian ini untuk mengetahui kemampuan 12 jenis Trichoderma spp., dalam menghambatpertumbuhan C. gloeosporioides. Pengujian ini terdiri dari uji antagonis menggunakan mode kulturganda dan uji Trichoderma spp. terhadap pertumbuhan C. gloeosporioides. Penghambatan yang palingtinggi sebesar 23,93 % isolat dari Trichoderma spp. asal Sinar Baru, Rantau Badauh, KalimantanSelatan. dan penghambatannya yang terendah 7,31 % isolat Trichoderma spp. asal Margasari,Kabupaten Tapin, Kalimantan Selatan.

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“…An environmentally-friendly control method is the use of biocontrol agents [7]. Trichoderma harzianum has been proven effective and widely used to control plant pathogens [8]. Rai et al [9] reported that T. harzianum can control the in vitro growth of Fusarium oxysporum f. sp.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol Delivery System of <i>Trichoderma harzianum</i> Formulated in Graphite and Silica Nano Particles to Control <i>Rhizoctonia solani</i> on Tomato Plants

Hersanti

Rizkie

Suryani

et al. 2021

MSF

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This paper reports the performance of a graphite and silica nanoparticles-based delivery system for T. harzianum in controlling the in vitro growth of R. solani and damping-off disease on tomato plants. The in vitro and in vivo experiments were arranged in the randomized complete block design. The in vitro treatment was a dual culture of R. solani and T. harzianum in the various components of formulation on PDA, i.e., T. harzianum + 5 wt.% graphite, T. harzianum + 1wt.% silica NPs., T. harzianum + 5 wt.% graphite + 1 wt.% silica nanoparticles, T. harzianum, 5 wt.% graphite, 1 wt.% silica nanoparticles, fungicide (mancozeb), and a control. The in vivo treatment included the application of T. harzianum in the same compositions as the in vitro treatment, except that there were two controls i.e., inoculated and noninoculated tomato plants with R. solani. T. harzianum by soaking tomato seeds in the formulation suspensions before planting. The results showed that all formulation compositions were able to inhibit the in vitro growth of R. solani. The inhibitions of the colony growth of R. solani caused by formulated and non-formulated T. harzianum were the same. This proved that graphite and silica NPs did not resist to the ability of T. harzianum in controlling R. solani, indicated that the formulation was promising to develop. However, the inhibition of damping-off disease incidence on tomato plants caused by formulated T. harzianum was the same as the non-formulated one only on day 7 after treatments. On days 14, 21, and 28, the inhibitions were lower than the non-formulated ones. It was suggested to reapply the formulation of T. harzianum in the soil at planting and several days after.

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KEMAMPUAN Trichoderma sp. DAN FILTRATNYA DALAM MENEKAN PERTUMBUHAN Sclerotium rolfsii SECARA IN VITRO

Cited by 4 publications

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Antagonism of Indigenous Fungi Collected from the Bamboo Clump against Fusarium sp., the Cause of Fusarium Wilt Disease in Garlic

Antagonism of Indigenous Fungi Collected from the Bamboo Clump against Fusarium sp., the Cause of Fusarium Wilt Disease in Garlic

Pengujian Dua Belas Jenis Trichoderma Untuk Mengendalikan Penyakit Antraknosa (Colletotrichum gloeosporioides) pada Bawang Merah (Allium ascalonicum L.)

Biocontrol Delivery System of <i>Trichoderma harzianum</i> Formulated in Graphite and Silica Nano Particles to Control <i>Rhizoctonia solani</i> on Tomato Plants

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