2015
DOI: 10.1074/jbc.m114.593707
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Key Importance of Small RNA Binding for the Activity of a Glycine-Tryptophan (GW) Motif-containing Viral Suppressor of RNA Silencing

Abstract: Background: GW/WG motif-containing viral suppressors of RNA silencing (VSRs) have been proposed to act through interaction with Argonaute (AGO) proteins. Results: The activity of a GW motif-containing VSR was found to rely on small RNA binding capability rather than on AGO interaction. Conclusion:Overlapping signals in VSRs may lead to misinterpretation of relevant molecular traits. Significance: Knowing primary target(s) of VSRs is critical for better understanding of the host-virus arms race.

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Cited by 46 publications
(85 citation statements)
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References 93 publications
(137 reference statements)
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“…A recent report also revealed that PLPV p37, besides acting as a CP, also functions as a VSR [26], paralleling results obtained with several carmoviruses [22,23,28].…”
Section: Genomic Organization and Coding Propertiessupporting
confidence: 48%
See 1 more Smart Citation
“…A recent report also revealed that PLPV p37, besides acting as a CP, also functions as a VSR [26], paralleling results obtained with several carmoviruses [22,23,28].…”
Section: Genomic Organization and Coding Propertiessupporting
confidence: 48%
“…Moreover, the complete PLPV genome was the first to be made available, and this virus is currently the best characterized among the tentative members of this proposed genus. To date, work performed on PLPV at the molecular level includes (i) sgRNA mapping [5], (ii) generation of infectious cDNA clones under the control of the T7 RNA polymerase promoter or 35S cauliflower mosaic virus promoter [6], (iii) analyses of gene function and translational mechanisms [8], (iv) analyses of molecular variability [7], and (v) identification of the VSR and its molecular interactions [26]. Though limited, data on the incidence of PLPV in cultivated geraniums in Spain have been also obtained [2].…”
Section: Introductionmentioning
confidence: 99%
“…Recently, Pelargonium line pattern virus (PLPV) P37 (a protein related to TCV P38, Fig. 4A) was shown to act as a VSR and to bind siRNA duplexes but not long dsRNA in N. benthamiana leaf extracts (Perez-Canamas and Hernandez 2015). Phylogenetic analysis of carmovirus P38 proteins indicates that TCV P38 is more similar to CCFV P38 than it is to PLPV P37 In parallel, the same, but 32 P-labeled template was incubated with HA-RDR6-enriched beads in the absence of [α-32 P]-UTP.…”
Section: Inhibition Of Risc Loading and Dsrna Processing Are Recentlymentioning
confidence: 99%
“…CP is a strong viral suppressor of RNA silencing (VSR; Chattopadhyay et al, 2015;Mérai et al, 2006;Pérez-Cañamás and Hernández, 2015;Qu et al, 2003;Thomas et al, 2003;Zhang et al, 2012). It is also required for cell-to-cell movement of TCV in N. benthamiana (Cohen et al, 2000;Li et al, 2009).…”
Section: Rna Silencing Is An Innate Antiviral Mechanism Conserved In mentioning
confidence: 99%