Kidney ischemia and reperfunsion syndrome: effect of lidocaine and local postconditioning

Yamaki, I; Pontes, Ruy Victor Simões; Costa, Felipe Lobato da Silva; Yamaki, Vítor Nagai; Teixeira, Renan Kleber Costa; Yasojima, Edson Yuzur; Brito, Marcus Vinicius Henriques

doi:10.1590/0100-69912016005012

Cited by 5 publications

(4 citation statements)

References 28 publications

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“…38 It is thought that through modulations in calcium and sodium homeostasis, Lidocaine is able to attenuate the harmful effects of IRI in the kidney. 39 In addition, lidocaine is known to cause vasodilation via blockade of sodium channels, especially at higher doses. 40 This could contribute to the preservation of renal blood flow during ischemia.…”

Section: Discussionmentioning

confidence: 99%

Pharmacological prevention of renal ischemia–reperfusion injury in a rat model

Deng

Yang³

et al. 2021

ANZ Journal of Surgery

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Introduction: Renal ischemia-reperfusion injury (IRI) can lead to significant morbidity and mortality. It remains a leading cause of acute kidney injury and is therefore an important issue in trauma and renal transplant surgery. Various pharmaceutical agents have been used in an attempt to dampen the harmful effects of IRI but few have been shown to be useful clinically. Riluzole, Lidocaine and Lamotrigine have been demonstrated to show antiischaemic properties in other organs; however, their use has not been tested in the kidneys. We investigated Riluzole, Lidocaine and Lamotrigine for their preventive effects of renal IRI using a rat model. Methods: Winstar rats (n = 48) were divided into four groups (n = 12 per group)-three treatment groups and one control group. Riluzole, Lidocaine and Lamotrigine were given prior to renal ischemia only (IO) or IRI. The degree of ischemia was measured by glutathione levels and a TUNEL assay was used to measure DNA fragmentation. Results: Riluzole, Lidocaine and Lamotrigine pre-treatment each resulted in statistically higher glutathione levels compared to controls (P = 0.002; P = 0.007 and P = 0.005, respectively). Riluzole and Lidocaine were also effective at preventing depletion of glutathione following IO (P = 0.007 and P = 0.014 respectively), while Lamotrigine was ineffective in IO (P = 0.71). The degree of DNA fragmentation seen on the TUNEL assay was markedly reduced in all three-drug groups in both IO and IRI. Discussion: Riluzole, Lidocaine and Lamotrigine all have anti-ischaemic effects in the rat kidney and can have potential therapeutic implications.

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Section: Discussionmentioning

confidence: 99%

Pharmacological prevention of renal ischemia–reperfusion injury in a rat model

Deng

Yang³

et al. 2021

ANZ Journal of Surgery

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“…Some authors suggest that humoral factors are released from the tissue submitted to intermittent vascular occlusion, as adenosine, bradykinin and opioids 24 . Thus, the activation of those effector signals allow interaction between remote organs and the hind limb, and starts intracellular response, for example activation of RISK and SAFE pathways 25 .…”

Section: Discussionmentioning

confidence: 99%

Remote ischemic conditioning improves rat brain antioxidant defense in a time-dependent mechanism

et al. 2021

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Purpose To clarify the best protocol for performing remote ischemic conditioning and to minimize the consequences of ischemia and reperfusion syndrome in brain, the present study aimed to evaluate different time protocols and the relation of the organs and the antioxidant effects of this technique. Methods The rat’s left femoral artery was clamped with a microvascular clamp in times that ranged from 1 to 5 minutes, according to the corresponding group. After the cycles of remote ischemic conditioning and a reperfusion of 20 minutes, the brain and the left gastrocnemius were collected. The samples were used to measure glutathione peroxidase, glutathione reductase and catalase levels. Results In the gastrocnemius, the 4-minute protocol increased the catalase concentration compared to the 1-minute protocol, but the latter increased both glutathione peroxidase and glutathione reductase compared to the former. On the other hand, the brain demonstrated higher catalase and glutathione peroxidase in 5-minute group, and the 3-minute group reached higher values of glutathione reductase. Conclusions Remote ischemic conditioning increases brain antioxidant capacity in a time-dependent way, while muscle presents higher protection on 1-minute cycles and tends to decrease its defence with longer cycles of intermittent occlusions of the femoral artery.

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“…The vascular anatomy of kidney is unique 22,30 where glomerulus receive blood supply prior to medullar area (tubulus), being very different from the heart or liver vascular anatomy; myocites have also different cell biology. Our findings that PER might confer protection to the glomerulus and tubules and POS only to the glomerulus, support that tissue conditioning techniques might work in a tissue dependent manner.…”

Section: ■ Discussionmentioning

confidence: 99%

Perconditioning combined with postconditioning on kidney ischemia and reperfusion

et al. 2017

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Purpose: To evaluate if combination of perconditioning and postconditioning provides improved renal protection compared to perconditioning alone in a model of renal reperfusion injury. Methods: Thirty rats were assigned into 6 groups: normality; sham; ischemia and reperfusion; postconditioning; perconditioning; perconditioning + postconditioning. Animals were subjected to right nephrectomy and left renal ischemia for 30 minutes. Postconditioning consisted of 3 cycles of 5 min renal perfusion followed by 5 min of renal ischemia after major ischemic period. Perconditioning consisted of 3 cycles of 5 min hindlimb ischemia followed by 5 min of hindlimb perfusion contemporaneously to renal major ischemic period. After 24 hours, kidney was harvested and blood collected to measure urea and creatinine. Results: Perconditioning obtained better values for creatinine and urea level than only postconditioning (p<0.01); performing both techniques contemporaneously had no increased results (p>0.05). Regarding tissue structure, perconditioning was the only technique to protect the glomerulus and tubules (p<0.05), while postconditioning protected only the glomerulus (p<0.05). Combination of both techniques shows no effect on glomerulus or tubules (p>0.05). Conclusions: Perconditioning had promising results on ischemia and reperfusion induced kidney injury, enhanced kidney function and protected glomerulus and tubules. There was no additive protection when postconditioning and perconditioning were combined.

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Kidney ischemia and reperfunsion syndrome: effect of lidocaine and local postconditioning

Abstract: a lidocaína não bloqueou os efeitos do pós-condicionamento na síndrome de isquemia e reperfusão renal, mas conferiu melhor na proteção glomerular quando aplicada em conjunto com o pós-condicionamento isquêmico.

Cited by 5 publications

References 28 publications

Pharmacological prevention of renal ischemia–reperfusion injury in a rat model

Pharmacological prevention of renal ischemia–reperfusion injury in a rat model

Remote ischemic conditioning improves rat brain antioxidant defense in a time-dependent mechanism

Perconditioning combined with postconditioning on kidney ischemia and reperfusion

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