Kinetic Colorimetric Measurement of Serum Lactate Dehydrogenase Activity

Babson, Arthur L.; Babson, Susan R.

doi:10.1093/clinchem/19.7.766

Cited by 107 publications

(49 citation statements)

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“…As reported: succinate dehydrogenase (Shelton & Rice, ), lactate dehydrogenase (Babson & Babson, ), G‐6‐Pase (Swanson, ), acid phosphatase (Wattiaux & De Duve, ), and 5′‐nucleotidase (Bodansiky & Schwartz, ).…”

Section: Methodsmentioning

confidence: 74%

Triacylglycerols of the seed oil ofLinum grandiflorumDesf.: Their composition, cytotoxicity, and hepatoprotective activity

et al. 2018

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Section: Methodsmentioning

confidence: 74%

Triacylglycerols of the seed oil ofLinum grandiflorumDesf.: Their composition, cytotoxicity, and hepatoprotective activity

et al. 2018

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“…They were homogenized by a high speed blinder in a solution of 0.9% NaCl, 5 mM TRIS-HCl buffer (pH 7.4). Extracts were collected after centrifugation for 30 minutes at 20 ºC, 15000xg, then LDH activity was analyzed in the clear supernatant, (Babson and Babson 1973). Polyacryamide gel was prepared as 5.5% in TRIS-HCl buffer, pH 8.6 (3mm inner diameter, 5cm length).…”

Section: Methodsmentioning

confidence: 99%

Lactate dehydrogenase isoenzyme pattern in the liver tissue of chemically-injured rats treated by combinations of diphenyl dimethyl bicarboxylate

Faddah¹,

Abdel-Hamid²,

Abul-Naga³

et al. 2007

J Appl Biomed

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The purpose of our study is to evaluate the protective effect of diphenyl dimethyl bicarboxylate (DDB) in combination with some antioxidants, namely vitamin C (V.C), vitamin E (V.E), and selenium (Se), in liver damage induced by carbon tetrachloride (0.2 ml/kg body weight). This was done by monitoring the liver total and fractional lactate dehydrogenase (LDH) activities. The results revealed a significant increase in the activity of liver total LDH activity in CCl4-intoxicated rats with a significant increase in both LDH3 and 4 and a significant decrease in LDH5. LDH2 disappeared after CCl4 treatment and neither DDB nor its combinations could restore this permanent change. DDB alone significantly decreased the CCl4-raised total LDH and LDH4, but still far from the control and failed to correct LDH3 and 5 variations. A combination of DDB and V.C, V.E and Se showed the best corrective potential in both total LDH and LDH3 activities, without correcting the increased LDH4, nor the decreased LDH5 isoenzyme. Although this combination was previously reported to correct liver function disturbances, it seems that CCl4 and consequently hepatitis C may induce some irreversible, non-curable changes by DDB or even by additional antioxidants. Its clinical usefulness seems to be through different metabolic pathways, not including correction of LDH disturbances, which necessitates additional investigation for other adjunct medicines for treating liver fibrosis in clinical practice.

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“…Percent cell lysis was determined by measuring the level of LDH, an enzyme that catalyzes the oxidation of lactate to pyruvate (Babson and Babson, 1973;Legrand et al, 1992). All sample aliquots were stored in 0.1 g/L saponin at <À708C until analysis.…”

Section: Cell Lysis Measurementsmentioning

confidence: 99%

Identification and prevention of antibody disulfide bond reduction during cell culture manufacturing

Trexler-Schmidt

Sargis

Chiu³

et al. 2010

Biotech & Bioengineering

102

139

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In the biopharmaceutical industry, therapeutic monoclonal antibodies are primarily produced in mammalian cell culture systems. During the scale-up of a monoclonal antibody production process, we observed excessive mechanical cell shear as well as significant reduction of the antibody's interchain disulfide bonds during harvest operations. This antibody reduction event was catastrophic as the product failed to meet the drug substance specifications and the bulk product was lost. Subsequent laboratory studies have demonstrated that cells subjected to mechanical shear release cellular enzymes that contribute to this antibody reduction phenomenon (manuscript submitted; Kao et al., 2009). Several methods to prevent this antibody reduction event were developed using a lab-scale model to reproduce the lysis and reduction events. These methods included modifications to the cell culture media with chemicals (e.g., cupric sulfate (CuSO(4))), pre- and post-harvest chemical additions to the cell culture fluid (CCF) (e.g., CuSO(4), EDTA, L-cystine), as well as lowering the pH and air sparging of the harvested CCF (HCCF). These methods were evaluated for their effectiveness in preventing disulfide bond reduction and their impact to product quality. Effective prevention methods, which yielded acceptable product quality were evaluated for their potential to be implemented at manufacturing-scale. The work described here identifies numerous effective reduction prevention measures from lab-scale studies; several of these methods were then successfully translated into manufacturing processes.

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Kinetic Colorimetric Measurement of Serum Lactate Dehydrogenase Activity

Cited by 107 publications

References 0 publications

Triacylglycerols of the seed oil ofLinum grandiflorumDesf.: Their composition, cytotoxicity, and hepatoprotective activity

Triacylglycerols of the seed oil ofLinum grandiflorumDesf.: Their composition, cytotoxicity, and hepatoprotective activity

Lactate dehydrogenase isoenzyme pattern in the liver tissue of chemically-injured rats treated by combinations of diphenyl dimethyl bicarboxylate

Identification and prevention of antibody disulfide bond reduction during cell culture manufacturing

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