Kinetic evaluation of muscle damage during exercise by calculation of amount of creatine kinase released

Volfinger, L.; Lassourd, V.; Michaux, J. M.; Jp, Braun; Toutain, P. L.

doi:10.1152/ajpregu.1994.266.2.r434

Cited by 60 publications

(68 citation statements)

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“…Warriss et al, 1998;Pé rez et al, 2002). Serum CK originates from striated muscle following an increase of membrane permeability that can be triggered for instance by physical exercise (Boyd, 1983;Schmidt and Schmidt, 1987;Volfinger et al, 1994). Since CK has a long half-life in plasma (5.2 6 1.3 h in pigs) (Bickhardt, 1969), the concentrations measured at slaughter reflect the environmental effects during a longer period preceding slaughter, including the response to transportation.…”

Section: Discussionmentioning

confidence: 99%

Stress neuroendocrine profiles in five pig breeding lines and the relationship with carcass composition

Foury

Geverink²,

Gil³

et al. 2007

Animal

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Stress neuroendocrine systems (hypothalamic-pituitary-adrenal axis and sympathetic nervous system) were studied in 100 female pigs from each of the five main genetic lines used in Europe for pork production: Pié train, Large White, Landrace, Duroc and Meishan. Levels of cortisol and catecholamines were measured in urine collected at the farm, after transportation to the slaughterhouse and the next morning before slaughter. With the exception of the Pié train line that showed intermediate levels of cortisol despite its extreme leanness, a significant positive relationship was found between basal cortisol levels and fatness, both across and within (except in Pié train and Duroc) lines. Basal cortisol levels were 2.46-fold higher in Meishan (20.46 ng/mg creatinine) than in Large White pigs (8.30 ng/mg creatinine), the two extreme breeds. Post-transportation levels were highest but proportional to basal levels, suggesting that the adrenal reactivity to adrenocorticotropic hormone is a major source of variability between lines. Levels of catecholamines were less variable between lines but correlated also with fatness, partlyviapartial correlations with cortisol levels. In serum collected at exsanguination, creatine kinase activity was correlated with muscularity across the five breeds. However, this was due to a much larger activity than expected in Pié train pigs, although all animals were negative for the allele of the ryanodine receptor gene responsible for stress sensitivity. Serum glucose levels were inversely related to fatness. These data show that the differences between breeds or lines can be utilised by cross-breeding and that this can lead to changes in stress hormones and in turn to some degree of changes in carcass traits.

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Section: Discussionmentioning

confidence: 99%

Stress neuroendocrine profiles in five pig breeding lines and the relationship with carcass composition

Foury

Geverink²,

Gil³

et al. 2007

Animal

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“…The increase of CK activity can be the result of muscle cell damage or increased cell membrane permeability. Only CK activity higher than 10 000 U/L presents some evidence of myolysis (Volfinger et al 1994). Therefore, in our case, the increased muscle cell membrane permeability caused the elevated CK activity.…”

Section: Resultsmentioning

confidence: 47%

“…These observations indicate that horses subject to regular but relatively short intensive exercises could have chronic muscle damage which results in a somewhat elevated CK level (about 200-300 U/L). Only prolonged endurance exercise (60 km or more) can result in very high levels of CK (1000-30 000 U/L) (Kerr & Snow 1983;Volfinger et al 1994;Adamu et al 2013). The increase of CK activity can be the result of muscle cell damage or increased cell membrane permeability.…”

Section: Resultsmentioning

confidence: 99%

Effect of dietary energy source on the plasma parameters of equine athletes trained in a deep water aqua treadmill

Vincze¹,

Szabó

Bakos

et al. 2016

Italian Journal of Animal Science

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The aim of the study was to test the effect of different dietary energy sources on several blood biochemical parameters on aqua treadmill trained show jumpers. Four horses in latin square arrangements consumed identical amounts of meadow hay, and four concentrates differing mainly in their energy source (control, starch from oat, oil from sunflower and sugar from sugar beet molasses) but providing the same amount of energy. One experimental period consisted of a 10 day adaptation and 4 day test period involving deep water aqua treadmill training. Blood samples were taken during and after the last aqua treadmill training and lactate, lactate dehydrogenase, creatine kinase, aspartate aminotransferase, glucose and triglycerides levels were determined from the plasma. The different dietary energy sources resulted in similar plasma lactate levels. The increased starch content of the feed resulted in significantly lower (p < 0.05) creatine kinase level at the end of the first walking section of aqua training. This result appeared later as a tendency (p 0.1). Horses fed sunflower oil as a main energy source had higher aspartate aminotransferase level after two hours of the aqua training. The plasma triglyceride concentration in the sunflower oil group tended (p < 0.1) to be lower at the end of aqua training; while one hour after the training it was significantly lower. The elevated level of creatine kinase and aspartate aminotransferase indicates that lactate does not correctly reflect the strenuousness of the aqua training. The dietary energy source modifies the metabolic response to aqua training, even if it is not considerable. ARTICLE HISTORY

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“…As microlesões acometidas pelo estresse mecânico causam liberação de enzimas sarcoplasmáticas na circulação, sendo as principais encontradas a creatina quinase, lactato desidrogenase, aspartato aminotransferase e mioglobina [2][3][4], o que sugere refletir uma significativa mudança na estrutura e permeabilidade do sarcolema. Por isso, dosagens plasmáticas dessas proteínas, em especial a creatina quinase, são utilizadas como biomarcadores de alteração muscular [33,36].…”

Section: Qual a Relação Existente Entre A Presença Da Enzima Ck No Saunclassified

“…Em contraste, a DMT é a sensação de desconforto e rigidez nos músculos que, muitas vezes depois de uma atividade física não habitual, normalmente aumenta a intensidade nas primeiras 24 horas depois do exercício, com picos tardios [1]. A hipótese mais plausível para explicar a instalação da dor muscular tardia relaciona-se com os eventos de microlesões celulares induzidos pelo exercício exaustivo [2][3][4]. Um marcador muito eficiente para verificar o grau de alteração da célula muscular ou da permeabilidade da membrana é a dosagem de enzimas sarcoplasmáticas no plasma, dentre elas, sobretudo, a creatina quinase (CK) [5].…”

Section: Introductionunclassified

Desmistificando a Ação Do Lactato Nos Eventos De Dor Muscular Tardia Induzida Pelo Exercício Físico: Proposta De Uma Aula Prática

Neto¹,

Melo²,

Filho³

et al. 2006

Rev. Ens. Bioq.

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RESUMONosso estudo tem como objetivo desmistificar, para alunos de graduação que cursam a disciplina Bioquímica do Exercício Físico, a ação do lactato nos eventos da dor muscular tardia (DMT) induzida pelo exercício físico. Para tanto, realizamos um exercício de corrida exaustiva (3 corridas intensivas de 250 metros, em pista oficial de 400 metros, com intervalos de recuperação ativa de 150 metros de caminhada). Participaram do exercício 5 alunos (3 homens e 2 mulheres) com idade média de 21±2 anos. A tentativa foi desenvolver uma atividade não habitual e exaustiva, a fim de elevar a concentração de lactato sangüínea e induzir um nível de desconforto muscular. Coletamos 3 mL de sangue antes do esforço físico (valor controle -CO), imediatamente após (T0h), duas horas após (T2h) e quatro horas após a realização da corrida (T4h), para obtermos, assim, as dosagens de lactato (em sangue total) e de creatina quinase (CK, no plasma). As análises de concentração de lactato no sangue foram realizadas através do aparelho Accutrend® Lactate (Roche) e as dosagens dos níveis de CK através do método reativo para determinação de quantidade plasmática (CK NAC -Método Cinético, Laborlab) por meio de espectrofotômetro. Houve um aumento significativo dos níveis de lactato no sangue imediatamente após a realização do exercício exaustivo, porém após o tempo T2h e T4h os valores encontraram-se nos níveis detectados para o grupo controle (CO), revelando uma estratégia de remoção deste metabólito. Pudemos observar que houve um comportamento idêntico quanto ao aumento de concentração de CK plasmática em todos os indivíduos, após 4 horas da realização do exercício exaustivo, com aumento significativo em T4h em comparação ao grupo controle. A concentração plasmática de lactato volta aos níveis pré-exercício 2h após a realização do exercício intenso e muito antes da instalação dos primeiros sintomas da dor muscular tardia. Concluímos que a elevação das dosagens plasmáticas de CK após o exercício exaustivo pode ser em decorrência de um aumento de permeabilidade ou perturbação mais efetiva do sarcolema, constituindo um primeiro evento participativo no desencadeamento da DMT.

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Kinetic evaluation of muscle damage during exercise by calculation of amount of creatine kinase released

Cited by 60 publications

References 0 publications

Stress neuroendocrine profiles in five pig breeding lines and the relationship with carcass composition

Stress neuroendocrine profiles in five pig breeding lines and the relationship with carcass composition

Effect of dietary energy source on the plasma parameters of equine athletes trained in a deep water aqua treadmill

Desmistificando a Ação Do Lactato Nos Eventos De Dor Muscular Tardia Induzida Pelo Exercício Físico: Proposta De Uma Aula Prática

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