Kinetic spectrophotometric determination of pravastatin in drug formulations via derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

Ashour, Safwan; Khateeb, Mouhammed

doi:10.1016/j.arabjc.2010.06.050

Cited by 12 publications

(7 citation statements)

References 27 publications

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“…4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) has been used as a derivatizing reagent for the determination of many pharmaceuticals such as pravastatin (24), fluoroquinolones (25) and domoic acid (26), saxagliptin and many pharmaceutical amines (27)(28)(29)(30)(31)(32)(33)(34). To date, nothing has been published concerning the fluorimetric determination of CBZ after a nucleophilic substitution reaction.…”

Section: Introductionmentioning

confidence: 99%

Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4‐chloro‐7‐‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl)

2015

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A sensitive and simple spectrofluorimetric method has been developed and validated for the determination of the anti-epileptic drug carbamazepine (CBZ) in its dosage forms. The method was based on a nucleophilic substitution reaction of CBZ with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in borate buffer (pH 9) to form a highly fluorescent derivative that was measured at 530 nm after excitation at 460 nm. Factors affecting the formation of the reaction product were studied and optimized, and the reaction mechanism was postulated. The fluorescence-concentration plot is rectilinear over the range of 0.6-8 µg/mL with limit of detection of 0.06 µg/mL and limit of quantitation of 0.19 µg/mL. The method was applied to the analysis of commercial tablets and the results were in good agreement with those obtained using the reference method. Validation of the analytical procedures was evaluated according to ICH guidelines.

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Section: Introductionmentioning

confidence: 99%

Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4‐chloro‐7‐‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl)

2015

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“…The mechanism of derivatization reactions may be interpreted as shown in Scheme 1 and 2. NBD‐Cl (Ashour and Khateeb 2011) has also been used in the determination of pravastatin sodium, based on the formation of colored product between PVS and NBD‐Cl, which is nearly the same mechanism as proposed above.…”

Section: Resultsmentioning

confidence: 95%

Utility of 4‐Chloro‐7‐Nitrobenzofurazan for the Spectrofluorimetric Determination of Butylated Hydroxyanisole and Propyl Gallate in Foodstuffs

Chen

Tai

et al. 2012

Journal of Food Science

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“…The proposed method was based on DGF derivatization with NBD-Cl. [27,28] Optimum conditions for analysis of the resultant fluorescent DGF complex in different matrices were optimized by employing full factorial experimental design using Minitab® software.…”

Section: Introductionmentioning

confidence: 99%

New spectrofluorimetric analysis of dapagliflozin after derivatization with NBD‐Cl in human plasma using factorial design experiments

et al. 2019

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A new validated spectrofluorimetric method was proposed for dapagliflozin (DGF) analysis in bulk, plexin its commercially available tablets and in spiked human plasma.The proposed spectrofluorimetric method depended on the formation of a fluorescent complex soluble in organic liquids by a substitution reaction between 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) reagent and DGF in aqueous buffered solution at pH 7. The fluorescence intensity was measured at 522 nm after excitation at 453 nm. The high selectivity of the proposed method allowed analysis of DGF in dosage form and human plasma samples with average recovery values of 99.84 ± 1.38% and 98.71 ± 1.80%, respectively, without any interference from matrix components. The calibration range was 50-1000 ng ml −1 . The limit of detection (LOD) and limit of quantitation (LOQ) were 14.24 ng ml −1 and 43.14 ng ml −1 , respectively. The estimated relative standard deviation values were lower than 2.0%, this showed the excellent precision at both levels. Factorial design was used to get the optimum method conditions for the analysis of the resulting DGF fluorescence complex in different matrices. The proposed method could be used in routine analysis of DGF in quality control laboratories. Also, it could be used to assay DGF in human plasma and be applied for pharmacokinetic investigation of DGF.

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Kinetic spectrophotometric determination of pravastatin in drug formulations via derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

Cited by 12 publications

References 27 publications

Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4‐chloro‐7‐‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl)

Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4‐chloro‐7‐‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl)

Utility of 4‐Chloro‐7‐Nitrobenzofurazan for the Spectrofluorimetric Determination of Butylated Hydroxyanisole and Propyl Gallate in Foodstuffs

New spectrofluorimetric analysis of dapagliflozin after derivatization with NBD‐Cl in human plasma using factorial design experiments

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