A cloud-point extraction (CPE) method using Triton X-114 (TX-114) nonionic surfactant was developed for the extraction and preconcentration of propyl gallate (PG), tertiary butyl hydroquinone (TBHQ), butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT) from edible oils. The optimum conditions of CPE were 2.5% (v/v) TX-114, 0.5% (w/v) NaCl and 40 min equilibration time at 50 °C. The surfactant-rich phase was then analyzed by reversed-phase high-performance liquid chromatography with ultraviolet detection at 280 nm, using a gradient mobile phase consisting of methanol and 1.5% (v/v) acetic acid. Under the studied conditions, 4 synthetic phenolic antioxidants (SPAs) were successfully separated within 24 min. The limits of detection (LOD) were 1.9 ng mL(-1) for PG, 11 ng mL(-1) for TBHQ, 2.3 ng mL(-1) for BHA, and 5.9 ng mL(-1) for BHT. Recoveries of the SPAs spiked into edible oil were in the range 81% to 88%. The CPE method was shown to be potentially useful for the preconcentration of the target analytes, with a preconcentration factor of 14. Moreover, the method is simple, has high sensitivity, consumes much less solvent than traditional methods, and is environment-friendly. Practical Application: The method established in this article uses less organic solvent to extract SPAs from edible oils; it is simple, highly sensitive and results in no pollution to the environment.
With the trend of digital transformation of enterprises, the use of Internet of Things (IoT) devices is increasing. IoT devices that are not protected by security measures have gradually become targets of attackers. Attackers use weak passwords and software vulnerabilities in the device to invade the device and control it to become a node of the botnet. The Mozi botnet was discovered in December 2019, and its attention has increased day by day, and its influence once exceeded Mirai. After a preliminary reverse analysis of the Mozi samples, we have continued to track the development and changes of the Mozi botnet since February 2021. First, through the in-depth analysis of the communication principles of the Mozi botnet and the distributed sloppy hash table protocol, we have proposed an in-depth analysis of the Mozi botnet. The active detection method of Mozi, through daily and continuous tracking of the number of Mozi nodes, is infinitely close to the boundary of the Mozi network. On the basis of the collected detection data, we give our conclusions on Mozi's node size, global geographic distribution, 24-hour global activity, equipment composition, and Mozi botnet countermeasures.Through this study, we found that the security of IoT devices around the world is not optimistic, and there is an urgent need to increase the security protection
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