Kinetic study of angiotensin converting enzyme activity by capillary electrophoresis after in-line reaction at the capillary inlet

Dyck, Sigrid Van; Vissers, Stijn; Schepdael, Ann Van; Hoogmartens, Jos

doi:10.1016/s0021-9673(02)01995-7

Cited by 45 publications

(36 citation statements)

References 31 publications

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“…EMMA has been successfully applied mainly in the field of enzyme activity study, including such enzymes, as lactate dehydrogenase [5], catechol-O-methyltransferase [13], phenol sulfotransferase [14], glycosidases [15], haloalkane deha-logenase [16], amine oxidase [17], g-glutamyltransferase [18], and angiotensin [19], and a number of inhibition studies [20][21][22][23]. The figures basing on EMMA results were in good agreement with the data obtained using other methods.…”

Section: Introductionsupporting

confidence: 65%

Enzymatic in‐capillary derivatization for glucose determination by electrophoresis with spectrophotometric detection

et al. 2008

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The following paper compares several procedures of in-capillary bienzymatic derivatization with regard to glucose determination with the use of glucose oxidase and horseradish peroxidase. The procedures discussed below include continuous contact in the capillary, plug-plug injection, and sequential injection with incubation in the capillary inlet. The reaction of hydrogen peroxide catalyzed by peroxidase was performed using two different substrates. The best results were achieved for nicotinamide adenine dinucleotide, reduced disodium salt (NADH) acting both as a chromogenic reagent and a substrate for peroxidase, while the method employed was sequential injection and incubation at the capillary inlet. The LOD was estimated to be 25 nM with a linear response up to 0.1 microM.

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Section: Introductionsupporting

confidence: 65%

Enzymatic in‐capillary derivatization for glucose determination by electrophoresis with spectrophotometric detection

et al. 2008

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“…Sensitive and rapid method has been developed for CZE determination of activity of ACE, an important regulator of blood pressure via conversion of inactive decapeptide angiotensin I into vaso-constrictor octapeptide angiotensin II. The method involves the in-capillary electrokinetic mixing of the substrate, synthetic peptide-hippuryl-His-Leu, with the enzyme, cleavage of substrate into hippuric acid and a dipeptide, followed by online CZE separation of substrate and products of the enzymatic reactions in BGE composed of 150 mM HEPES, pH 8.0 [269]. ACE activity is evaluated by the quantitation of one of the two products, hippuric acid, detected at 230 nm.…”

Section: Monitoring Of Chemical and Enzymatical Reactions And Physicamentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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The article gives a comprehensive review on the recent developments in the applications of high-performance capillary electromigration methods, zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. The article presents new approaches to the theoretical description and experimental verification of electromigration behavior of peptides, covers the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample preparation, suppression of peptide adsorption, new developments in individual separation modes, and new designs of detection systems. Several types of applications of capillary electromigration methods to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

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“…same purpose in at-inlet [15] and transverse diffusion of laminar flow profiles (TDLFP) [16] methodologies. The TDLFP methodology, primarily introduced by Krylov et al, was recently modified by our group to provide the reaction mixture with a uniform concentration of reactants.…”

Section: Introductionmentioning

confidence: 99%

Combination of on‐line CE assay with MS detection for the study of drug metabolism by cytochromes P450

et al. 2015

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A new CE-MS method with enzymatic reaction inside the capillary was developed for the study of drug metabolism by cytochromes P450. This automated method, based on the transverse diffusion of laminar flow profiles methodology, is comprised of the injection of substrates and enzyme, their mixing, incubation, and separation of the reaction products, all performed by CE, and their detection, identification, and quantification by MS. The developed and validated method was finally used to conduct a kinetic study of cytochrome P450 isoform 2C9 or human liver microsomes with diclofenac in order to demonstrate its practical functionality. All the estimated kinetic values--apparent Michaelis constants and apparent maximum reaction velocities were in agreement with literature data obtained using other techniques. In addition, the consumption of reactants was in the tens of nL per analysis. The method's usability was further demonstrated on tolbutamide, the other probe substrate of cytochrome P450 isoform 2C9. As a result, the method is conceptually applicable for the screening of any other cytochrome P450 isoform and its substrates and inhibitors after adapting the incubation and separation conditions.

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Kinetic study of angiotensin converting enzyme activity by capillary electrophoresis after in-line reaction at the capillary inlet

Cited by 45 publications

References 31 publications

Enzymatic in‐capillary derivatization for glucose determination by electrophoresis with spectrophotometric detection

Enzymatic in‐capillary derivatization for glucose determination by electrophoresis with spectrophotometric detection

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Combination of on‐line CE assay with MS detection for the study of drug metabolism by cytochromes P450

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