Kinetics of blood flow during healing of excisional full‐thickness skin wounds in pigs as monitored by laser speckle perfusion imaging

Stewart, Catherine J.; Gallant‐Behm, Corrie L.; Forrester, Kevin; Tulip, J.; Hart, David A.; Bray, Robert C.

doi:10.1111/j.0909-752x.2006.00157.x

Cited by 34 publications

(31 citation statements)

References 24 publications

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“…[23][24][25][26][27][28] Boas's group has been particularly active in this area and has also used the technique in an investigation into migraines. 29 Other medical applications have included microcirculation investigations, [30][31][32] dentistry, 33 wound and burn assessment, [34][35][36] and a return to ophthalmological problems. [37][38][39][40] Nonmedical applications have included measuring the velocity of vehicles 41,42 and monitoring the drying of paint.…”

Section: Some Recent Work On Laser Speckle Contrast Imagingmentioning

confidence: 99%

Laser speckle contrast imaging: theoretical and practical limitations

Briers¹,

Duncan²,

Hirst³

et al. 2013

J. Biomed. Opt

465

324

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Abstract. When laser light illuminates a diffuse object, it produces a random interference effect known as a speckle pattern. If there is movement in the object, the speckles fluctuate in intensity. These fluctuations can provide information about the movement. A simple way of accessing this information is to image the speckle pattern with an exposure time longer than the shortest speckle fluctuation time scale-the fluctuations cause a blurring of the speckle, leading to a reduction in the local speckle contrast. Thus, velocity distributions are coded as speckle contrast variations. The same information can be obtained by using the Doppler effect, but producing a two-dimensional Doppler map requires either scanning of the laser beam or imaging with a high-speed camera: laser speckle contrast imaging (LSCI) avoids the need to scan and can be performed with a normal CCD-or CMOS-camera. LSCI is used primarily to map flow systems, especially blood flow. The development of LSCI is reviewed and its limitations and problems are investigated.

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Section: Some Recent Work On Laser Speckle Contrast Imagingmentioning

confidence: 99%

Laser speckle contrast imaging: theoretical and practical limitations

Briers¹,

Duncan²,

Hirst³

et al. 2013

J. Biomed. Opt

465

324

View full text Add to dashboard Cite

show abstract

“…Although LSCI with a temporal processing scheme for contrast calculation can distinguish microvessels, 13 the vessel contrast can only be improved at the expense of sensitivity to blood flow. Although LSCI has previously been used to image angiogenic events in tumors 3 and wounds, 14 no study has demonstrated or achieved robust flow measurements at a high spatial resolution, as may enable reliable longitudinal assessment of angiogenesis using LSCI. We hypothesized that acquiring and combining LSCI data at multiple exposure times will serve the dual purpose of making blood-flow estimates more robust, as well as improving the CNR over a wide range of physiological blood flows, thus enabling sequential monitoring of the angiogenic microenvironment.…”

Section: Introductionmentioning

confidence: 99%

Multiexposure laser speckle contrast imaging of the angiogenic microenvironment

et al. 2011

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Abstract.We report the novel use of laser speckle contrast imaging (LSCI) at multiple exposure times (meLSCI) for enhanced in vivo imaging of the microvascular changes that accompany angiogenesis. LSCI is an optical imaging technique that can monitor blood vessels and the flow therein at a high spatial resolution without requiring the administration of an exogenous contrast agent. LSCI images are obtained under red (632 nm) laser illumination at seven exposure times (1-7 ms) and combined using a curve-fitting approach to obtain high-resolution meLSCI images of the rat brain vasculature. To evaluate enhancement in in vivo imaging performance, meLSCI images are statistically compared to individual LSCI images obtained at a single exposure time. We find that meLSCI reduced the observed variability in the LSCI-based blood-flow estimates by 30% and improved the contrast-to-noise ratio in regions with high microvessel density by 41%. The ability to better distinguish microvessels, makes meLSCI uniquely suited to longitudinal imaging of changes in the vascular microenvironment induced by pathological angiogenesis. We demonstrate this utility of meLSCI by sequentially monitoring, over days, the microvascular changes that accompany wound healing in a mouse ear model. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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“…Although specular reflection effects may be disregarded with the addition of polarising filters, static tissue reduces the signal-tonoise (SNR) ratio [114][115][116]. These factors contribute to the confinement of the speckle technique to superficial measurements, with deeper measurements requiring the necessity of being performed on surgically exposed tissue.…”

Section: Laser Speckle Imagingmentioning

confidence: 99%

‘Go with the flow ’: A review of methods and advancements in blood flow imaging

Daly

Leahy

2012

Journal of Biophotonics

110

108

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Physics has delivered extraordinary developments in almost every facet of modern life. From the humble thermometer and stethoscope to X‐Ray, CT, MRI, ultrasound, PET and radiotherapy, our health has been transformed by these advances yielding both morphological and functional metrics. Recently high resolution label‐free imaging of the microcirculation at clinically relevant depths has become available in the research domain. In this paper, we present a comprehensive review on current imaging techniques, state‐of‐the‐art advancements and applications, and general perspectives on the prospects for these modalities in the clinical realm. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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Kinetics of blood flow during healing of excisional full‐thickness skin wounds in pigs as monitored by laser speckle perfusion imaging

Cited by 34 publications

References 24 publications

Laser speckle contrast imaging: theoretical and practical limitations

Laser speckle contrast imaging: theoretical and practical limitations

Multiexposure laser speckle contrast imaging of the angiogenic microenvironment

‘Go with the flow ’: A review of methods and advancements in blood flow imaging

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