Kinetics of combination of O2 and CO with human hemoglobin F in cells and in solution

Holland, Robert A.B.

doi:10.1016/0034-5687(67)90061-8

Cited by 23 publications

(4 citation statements)

References 21 publications

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“…The similar mean thickness of neonatal and adult RBC corresponds to the finding of equal rates of oxygen uptake for neonatal and adult RBC (8). This also indicates that the decreased surface area-to-volume ratio of neonatal RBC does not impair oxygen uptake, which contradicts the calculations of Jones (10).…”

Section: Discussionsupporting

confidence: 57%

Geometry of Neonatal and Adult Red Blood Cells

Linderkamp

Meiselman

1983

Pediatr Res

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SummaryRed blood cell (RBC) geometry is a determinant of RBC deformability, survival time, osmotic resistance, and oxygen uptake. Because accurate information on the geometry of neonatal RBC appears lacking, a micropipette technique was employed to measure surface area and volume of individual neonatal and adult RBC. In addition, RBC diameter was determined microscopically. Abbreviation RBC, red blood cellAccurate knowledge about the geometry of red blood cells (RBC) is important for the study of deformability, survival time, osmotic resistance, and oxygen uptake of RBC (5). The geometry of RBC is defined by measureable dimensions as the cellular volume, membrane surface area, cell diameter and thickness, and by indices calculated from measured values. These calculated indices include the surface area index (actual surface area divided by area of a sphere of same volume) and the swelling index (maximal volume which a RBC can achieve by swelling divided by actual volume).The surface area index is a measure of the excess surface area beyond that required to enclose the cellular volume. A surface area index of 1.39 [the normal value for adult RBC (13)J indicates an excess surface area of 39%. RBC need excess membrane surface area to deform and to adopt various shapes during their passage through the microcirculation. A membrane-bound sphere possesses the minimal surface area required to enclose its volume and therefore is relatively rigid because deformation can only occur via increased membrane surface area.The swelling index is a measure of the swelling capacity of RBC. A swelling index of 1.62 [the normal value for adult RBC (13)] indicates that the volume of a RBC can increase by 62% before it reaches spherical shape and then hemolyzes. The swelling index is related to the osmotic resistance which is an indirect measure of the swelling ability of RBC.Another calculated RBC geometric property is the minimum cylindrical diameter which a cell can assume in a small cylindrical channel. This parameter indicates the minimal diameter of a capillary through which a RBC can pass without a change in its volume. The minimum cylindrical diameter is an important determinant of the flow behavior of RBC in the microcirculation. The minimum cylindrical diameter is also a major determinant of the flow rate through micropore filters and the pressure required to aspirate RBC into small micropipettes (5).The volume of RBC in full-term newborn infants is about 20% higher than in adults and the diameter of neonatal RBC is increased by about 10% compared to adults (16, 18). Riegel et al.(18) computed the surface area of neonatal RBC from measured diameters of dried erythrocytes and calculated mean cell thickness using a disc model. This assumption of a disc model, however, results in an overestimation of the surface area (5). Direct measurements of the surface area and thus calculated values of the surface area index, the swelling index, and the minimum cylindrical diameter do not appear to exist for neonatal RBC.The present study w...

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Section: Discussionsupporting

confidence: 57%

Geometry of Neonatal and Adult Red Blood Cells

Linderkamp

Meiselman

1983

Pediatr Res

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“…a and b ) differ substantially between equations. These differences are due to the fact that studies aimed at determining the correct θ CO equation have been performed under varying test conditions, such as different blood sources and blood pH (Holland, 1967; Reeves and Park, 1992; Roughton and Forster, 1957; Stam et al, 1991; Te Nijenhuis et al, 1996).

\frac{1}{θ_{italicCO}} = a * P_{cap} O_{2} + b

…”

Section: Introductionmentioning

confidence: 99%

Optimizing the calculation of D M,CO and V C via the single breath single oxygen tension DL CO/NO method

Coffman

Taylor

Carlson

et al. 2016

Respiratory Physiology & Neurobiology

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Alveolar-capillary membrane conductance (DM,CO) and pulmonary-capillary blood volume (VC) are calculated via lung diffusing capacity for carbon monoxide (DLCO) and nitric oxide (DLNO) using the single breath, single oxygen tension (single-FiO2) method. However, two calculation parameters, the reaction rate of carbon monoxide with blood (θCO) and the DM,NO/DM,CO ratio (α-ratio), are controversial. This study systematically determined optimal θCO and α-ratio values to be used in the single-FiO2 method that yielded the most similar DM,CO and VC values compared to the ‘gold-standard’ multiple-FiO2 method. Eleven healthy subjects performed single breath DLCO/DLNO maneuvers at rest and during exercise. DM,CO and VC were calculated via the single-FiO2 and multiple-FiO2 methods by implementing seven θCO equations and a range of previously reported α-ratios. The RP θCO equation (Reeves and Park, Respiration physiology 88:1–21, 1992.) and an α-ratio of 4.0–4.4 yielded DM,CO and VC values that were most similar between methods. The RP θCO equation and an experimental α-ratio should be used in future studies.

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“…This is largely due to the hydrophobicity of the membrane interior. However, this barrier property cannot be automatically extended to the permeation of small solutes such as oxygen, even though some experimental data suggest this to be true [1][2][3][4][5][6][7]. The overall conclusion from recent studies of oxygen transport across model and cell plasma membranes is that membranes are not barriers to oxygen transport into the cell and mitochondrion, and created oxygen concentration differences across these membranes at physiological conditions are negligible (see Review [8] and references therein).…”

Section: Introductionmentioning

confidence: 99%

Oxygen permeability of the lipid bilayer membrane made of calf lens lipids

Widomska

Raguž

Subczynski

2007

Biochimica et Biophysica Acta (BBA) - Biomembranes

114

144

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The oxygen permeability coefficient across the membrane made of the total lipid extract from the plasma membrane of calf lens was estimated from the profile of the oxygen transport parameter (local oxygen diffusion-concentration product) and compared with those estimated for membranes made of an equimolar 1-palmitoyl-2-oleoylphosphatidylcholine/cholesterol (POPC/Chol) mixture and of pure POPC. Profiles of the oxygen transport parameter were obtained by observing the collision of molecular oxygen with nitroxide radical spin labels placed at different depths in the membrane using the saturation-recovery EPR technique and were published by us earlier (J. Widomska, M. Raguz, J. Dillon, E. R. Gaillard, W. K. Subczynski, Biochim. Biophys. Acta. 1768 (2007) 1454-1465). At 35 degrees C, the estimated oxygen permeability coefficients were 51.3, 49.7, and 157.4 cm/s for lens lipid, POPC/Chol, and POPC membranes, respectively (compared with 53.3 cm/s for a water layer with the same thickness as a membrane). Membrane permeability significantly decreases at lower temperatures. In the lens lipid membrane, resistance to the oxygen transport is located in and near the polar headgroup region of the membrane to the depth of the ninth carbon, which is approximately where the steroid-ring structure of cholesterol reaches into the membrane. In the central region of the membrane, oxygen transport is enhanced, significantly exceeding that in bulk water. It is concluded that the high level of cholesterol in lens lipids is responsible for these unique membrane properties.

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Kinetics of combination of O2 and CO with human hemoglobin F in cells and in solution

Cited by 23 publications

References 21 publications

Geometry of Neonatal and Adult Red Blood Cells

Geometry of Neonatal and Adult Red Blood Cells

Optimizing the calculation of D M,CO and V C via the single breath single oxygen tension DL CO/NO method

Oxygen permeability of the lipid bilayer membrane made of calf lens lipids

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