Klorin e6’nın Etanol yardımıyla Metisiline Dirençli Stafilokok aureus üzerindeki Fotodinamik Etkisi

Abstract

Aim: The random use of antimicrobials for years has led to bacterial DNA mutation and a result of that, bacteria have become resistant to antibiotics. Methicillin-resistant Staphylococcus aureus (MRSA) is among these types of resistant bacteria that can easily infect when the immune system of the host is suppressed, and it significantly delays the wound healing. Different treatment methods are being investigated to overcome this problem. Antimicrobial photodynamic therapy is a candidate to become an alternative treatment for the destruction of MRSA. The aim of this study was to investigate the effect of chlorin e6 for the photoinactivation of MRSA and the synergetic role of ethanol in this mechanism. Methods: 655 nm laser light and Chlorin e6 as photosensitizer were examined for the photoinactivation of MRSA. Besides, 20% ethanol was used to increase the total antimicrobial efficacy with lower light energy densities and photosensitizer concentrations. The colony counting method was used to determine viable bacterial cells after each application. Results: 25 J/cm2 energy density with 20 μM Chlorin e6 and 50 J/cm2 energy density with 10 μM Chlorin e6 showed the highest bactericidal activity. When 20% ethanol was used as an adjuvant, 25 J/cm2 energy dose with 2 μM Chlorin e6 resulted in a better killing effect. Conclusion: Chlorin e6-mediated photodynamic therapy was successful to destroy MRSA and the addition of ethanol provided the opportunity to obtain higher antibacterial activity with lower light intensities and photosensitizer concentrations.

Comparison of the effect of ethanol and potassium iodide in antibacterial photodynamic therapy on gram negative pathogens

“…The Ce6 solution was prepared together with absolute EtOH to obtain a solution containing 20% EtOH. The concentration of EtOH was defined as 20%, because it was proved previously that this concentration was effective to enhance the photodynamic action on pathogens [11]. KI pellets were dissolved in sterile dH2O to obtain 100 mM KI solution and it was used as a potentiator in aPDT.…”

“…Ce6 absorbs 630-680 nm of wavelengths strongly [10]. For this reason, red part of the visible spectrum is an ideal choice to activate Ce6 [11]. Besides, the wavelengths around 600-nm is in therapeutic window in which the wavelengths can be safely used for the treatment of various diseases without giving serious harm to the biological tissues.…”