Knockdown of Long Non-Coding RNA NEAT1 Inhibits Proliferation and Invasion and Induces Apoptosis of Osteosarcoma by Inhibiting miR-194 Expression

Abstract

PurposeLong non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) has been implicated as an oncogene in the development and progression of osteosarcoma. This study aims to explore the mechanism of NEAT1 in osteosarcoma.Materials and MethodsExpressions of NEAT1 and miR-194 in osteosarcoma tissues and cells were detected by quantitative real-time PCR. The effects of NEAT1 knockdown or miR-194 overexpression on cell proliferation, invasion, and apoptosis were determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide (MTT) assay, transwell invasive assay, and flow cytometry analysis, respectively. Luciferase reporter assay was performed to observe the possible interaction between NEAT1 and miR-194.ResultsNEAT1 was upregulated and miR-194 was downregulated in osteosarcoma tissues and cells. Knockdown of NEAT1 or overexpression of miR-194 suppressed proliferation and invasion and induced apoptosis of osteosarcoma cells in vitro. Luciferase reporter assay validated that NEAT1 could interact with miR-194 and negatively modulated its expression. Furthermore, inhibition of miR-194 reversed the suppression of proliferation and invasion and the promotion of apoptosis induced by NEAT1 depletion in osteosarcoma cells.ConclusionKnockdown of NEAT1 suppressed proliferation and invasion and induced apoptosis in osteosarcoma cells by inhibiting miR-194 expression.

Knockdown long noncoding RNA nuclear paraspeckle assembly transcript 1 suppresses colorectal cancer through modulating miR‐193a‐3p/KRAS

“…In general, most researches concerning NEAT1 oncogenic mechanism applied NEAT1 RNA interference or other gene silencing techniques, including but not limited to short hairpin (sh) RNA and antisense oligonucleotides. For instance, NEAT1’s other targets, miR‐129/CTBP2, miR‐335‐3p/c‐met, miR‐107/CDK6, miR‐194, and hnRNP A2were verified through interrupting NEAT1; meantime, Oct4 was proved as an upstream of NEAT1 with the depletion of NEAT1. It could be achieved to inhibit cancer cell progression through disrupting NEAT1, and impaired capacity of proliferation, migration, invasion, and enhanced apoptosis was observed in assorted cancer cell lines.…”

Depression of lncRNA NEAT1 Antagonizes LPS-Evoked Acute Injury and Inflammatory Response in Alveolar Epithelial Cells via HMGB1-RAGE Signaling

“…Recently, lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) has become a subject of interest due to its pleiotropic function in diseases. NEAT1's role in cancer progression has been studied [9,10]. Increasing research also has implicated NEAT1 in inflammation-related diseases.…”

MALAT1 knockdown inhibits hypopharyngeal squamous cell carcinoma malignancy by targeting microRNA‑194

“…microRNAs (miRNAs/miRs) are a type of short non-coding RNAs (18-25 nucleotides in length) that regulate post-transcriptional gene expression by affecting the stability and translation of target mRNAs (15). miR-194 serves roles in metabolic diseases (16,17), dermatosis (8), the inflammatory response (18) and neoplasms (19,20). miR-194 is also involved in multiple aspects of skeletal muscle glucose metabolism by regulating AKT, glycogen synthase kinase 3 and oxidative phosphorylation (16).…”