Road Traffic safety and Traffic rules refers to the methods and measures used to prevent road users from being killed or seriously injured which are found to be higher in young adults which is directly or indirectly associated with level of awareness of an individual. This study aimed to find out the awareness on traffic rules and safety measures among Bachelor level students. A descriptive cross-sectional study was conducted among randomly selected 250 bachelor level students from selected colleges of Bharatpur, Chitwan that used structured questionnaire administered by researchers for collecting the data. Obtained data were analyzed in Statistical Package for Social Science (SPSS) version 20, using descriptive and inferential statistics. The result of this study showed that more than half (59.6%) of the students had adequate level of awareness regarding traffic rules and safety measures. Statistically significant association was found between awareness regarding Traffic rules and Safety measures with sex, personal experience/involvement in RTA and educational experience or training regarding RTA/Traffic rules/Road safety measures. It is concluded that nearly half of the students have inadequate awareness regarding road traffic rules and safety measures. Awareness is the major contributing factor for RTA prevention. Hence, all the stake holders, Ministry of Education, Ministry of Health, local government bodies, various government and private colleges need to expand further awareness programmes regarding traffic rules and safety measures to prevent the morbidity and mortality due to the accidents.