Korean Traditional Medicine (Jakyakgamcho-tang) Ameliorates Colitis by Regulating Gut Microbiota

Seo, Seung-Ho; Unno, Tatsuya; Park, Seong-Eun; Kim, Eunju; Lee, Yu‐Mi; Na, Chang-Su; Son, Hong-Seok

doi:10.3390/metabo9100226

Cited by 13 publications

(11 citation statements)

References 87 publications

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“…To verify whether S24-7 affects body weight through its metabolites, we performed correlation analysis and identified several anionic and cationic metabolites that were related to the abundance of S24-7. Similarly, it has been found that S24-7 is related to L-Valine, Leucine, and Glutaric acid, which are involved in the metabolism of amino acids and low cholesterol, low density lipoprotein (LDL), and fatty acid contents [22]. Sanguinetti et al [20] also observed that S24-7 correlated negatively with L-Valine, L-Leucine, Glutaric acid, and Metaxalone, which was similar to our result.…”

Section: S24-7 Might Affect the Weight Changes Of Offspring Through Different Metabolic Pathwayssupporting

confidence: 91%

Effects of S24–7 on the weight of progeny rats after exposure to ceftriaxone sodium during pregnancy

et al. 2021

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Antibiotic exposure during pregnancy will adversely affect the growth of offspring; however, this remains controversial and the mechanism is poorly understood. To study this phenomenon, we added ceftriaxone sodium to the drinking water of pregnant rats and continuously monitored the body weight of their offspring. The results showed that compared with the control group, the offspring exposed to antibiotics during pregnancy had a higher body weight up to 3 weeks old but had a lower body weight at 6 weeks old. To determine the role of the gut microbiota and its metabolites in the growth of offspring, we collected feces for sequencing and further established that the experimental group has a different composition ratio of dominant bacteria at 6 week old, among which S24–7 correlated negatively with body weight and the metabolites that correlated with body weight-related unique flora were L-Valine, L-Leucine, Glutaric acid, N-Acetyl-L-glutamate, and 5-Methylcytosine. To further explore how they affect the growth of offspring, we submitted these data to Kyoto Encyclopedia of Genes and Genomes website for relevant pathway analysis. The results showed that compared with the control, the following metabolic pathways changed significantly: Valine, leucine, and isoleucine biosynthesis; Protein digestion and absorption; and Mineral absorption. Therefore, we believe that our findings support the conclusion that ceftriaxone sodium exposure in pregnancy has a long-lasting adverse effect on the growth of offspring because of an imbalance of gut microbiota, especially S24–7, via different metabolic pathways.

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Section: S24-7 Might Affect the Weight Changes Of Offspring Through Different Metabolic Pathwayssupporting

confidence: 91%

Effects of S24–7 on the weight of progeny rats after exposure to ceftriaxone sodium during pregnancy

et al. 2021

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“…Taken together, the results suggested that LA might ameliorate UC through adjusting intestinal barrier-related bacteria. Furthermore, low-dose LA inhibited the decreased Allobaculum induced by DSS, which was consistent with a previous report [ 40 ]. However, our correlation analysis showed that Allobaculum was positively correlated with IL-6.…”

Section: Discussionsupporting

confidence: 93%

The Regulatory Effects of Licochalcone A on the Intestinal Epithelium and Gut Microbiota in Murine Colitis

et al. 2021

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The gut epithelium is a mechanical barrier that protects the host from the luminal microenvironment and interacts with the gut microflora, which influences the development and progression of ulcerative colitis (UC). Licochalcone A (LA) exerts anti-inflammatory effects against UC; however, whether it also regulates both the gut barrier and microbiota during colitis is unknown. The current study was conducted to reveal the regulatory effects of LA on the intestinal epithelium and gut microflora in C57BL/6 mice subjected to dextran sodium sulfate (DSS). Sulfasalazine (SASP) was used as the positive control. Results of clinical symptoms evaluation, hematoxylin, and eosin (H&E) staining, and enzyme-linked immunosorbent (ELISA) assays showed that LA significantly inhibited DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and gut inflammation. Additionally, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and immunohistochemical (IHC) analysis showed that LA maintained the integrity of the intestinal barrier by suppressing cell apoptosis and preserving the expression of tight junction (TJ) proteins. Notably, the optimal dose of LA for gut barrier preservation was low, while that for anti-inflammatory effects was high, indicating that LA might preserve gut barrier integrity via direct effects on the epithelial cells (ECs) and TJ proteins. Furthermore, 16S rRNA analysis suggested that the regulatory effect of LA on the gut microbiota differed distinctly according to dose. Correlation analysis indicated that a low dose of LA significantly modulated the intestinal barrier-associated bacteria as compared with a moderate or high dose of LA. Western blot (WB) analysis indicated that LA exhibited anti-UC activity partly by blocking the mitogen-activated protein kinase (MAPK) pathway. Our results further elucidate the pharmacological activity of LA against UC and will provide valuable information for future studies regarding on the regulatory effects of LA on enteric diseases.

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“…Similarly, it has been found that S24-7 is related to L-Valine, Leucine, and Glutaric acid, which are involved in the metabolism of amino acids and low cholesterol, low density lipoprotein (LDL), and fatty acid contents (Seo et al, 2019). Sanguinetti et al (Sanguinetti et al, 2018) also observed that S24-7 correlated negatively with L-Valine, L-Leucine, Glutaric acid, and Metaxalone, which was similar to our result.…”

Section: S24-7 Might Affect the Weight Changes Of Offspring Through Different Metabolic Pathwayssupporting

confidence: 90%

Effects of S24-7 on the weight of progeny rats after exposure to ceftriaxone sodium during pregnancy

Yang

Tang

Wen

et al. 2021

Preprint

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Antibiotic exposure during pregnancy will adversely affect the growth of offspring; however, this remains controversial and the mechanism is poorly understood. To study this phenomenon, we added ceftriaxone sodium to the drinking water of pregnant rats and continuously monitored the body weight of their offspring. The results showed that compared with the control group, the offspring exposed to antibiotics during pregnancy had a higher body weight up to 3 weeks old but had a lower body weight at 6 weeks old. To determine the role of the gut microbiota and its metabolites in the growth of offspring, we collected feces for sequencing and further established that the experimental group has a different composition ratio of dominant bacteria at 6 week old, among which S24-7 correlated negatively with body weight and the metabolites that correlated with body weight-related unique flora were L-Valine, L-Leucine, Glutaric acid, N-Acetyl-L-glutamate, and 5-Methylcytosine. To further explore how they affect the growth of offspring, we submitted these data to Kyoto Encyclopedia of Genes and Genomes website for relevant pathway analysis. The results showed that compared with the control, the following metabolic pathways changed significantly: Valine, leucine, and isoleucine biosynthesis; Protein digestion and absorption; and Mineral absorption. Therefore, we believe that our findings support the conclusion that ceftriaxone sodium exposure in pregnancy has a long-lasting adverse effect on the growth of offspring because of an imbalance of gut microbiota, especially S24-7, via different metabolic pathways.

show abstract

Korean Traditional Medicine (Jakyakgamcho-tang) Ameliorates Colitis by Regulating Gut Microbiota

Cited by 13 publications

References 87 publications

Effects of S24–7 on the weight of progeny rats after exposure to ceftriaxone sodium during pregnancy

Effects of S24–7 on the weight of progeny rats after exposure to ceftriaxone sodium during pregnancy

The Regulatory Effects of Licochalcone A on the Intestinal Epithelium and Gut Microbiota in Murine Colitis

Effects of S24-7 on the weight of progeny rats after exposure to ceftriaxone sodium during pregnancy

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