Kultivasi Fungi Mfw-01-08 yang Diisolasi dari Ascidia Aplidium longithorax dan Uji Aktivitas Sitotoksiknya Terhadap Sel Kanker Payudara T47D

Nursid, Muhammad; Pratitis, Asri; Chasanah, Ekowati

doi:10.15578/jpbkp.v5i2.412

Cited by 8 publications

(6 citation statements)

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“…Production of anticancer by Aspergillus ustus was reported to be 2 wk in MFM media, while when cultivated at MEB for 6 wk, the fungi produced antioxidant (Pratitis et al, 2010). Nursid et al (2010) also reported the potency of fungi MFW 01-08 to produce anticancer when cultivated at MEB media for 5 wk. This fungi was isolated from Ascidia Aplidium longithorax from Wakatobi waters.…”

Section: Resultsmentioning

confidence: 95%

“…This fungi has been proven to be active against cancer cell line of MCF-7 but not against H460 and SF 268 cells (Chasanah et al, 2009). Anticancer compound from fungi Aspergillus ustus extract has been reported by Pratitis et al (2010), while extract the same bioactivity from fungi MFW 01-08, isolated from ascidian Aplidium longithorax, has been reported by Nursid et al (2010). Previous work on the screening on fungi associated with marine sponge from Wakatobi waters showed that MFW 23-08 has been proven to be one of the potential fungi which was active against HeLa cell line and contained potential antioxidant (Pratitis et al, 2009).…”

Section: Introductionmentioning

confidence: 86%

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Identification and Cultivation of MFW 23-08 Isolated From Marine Sponges for Bioactive Compound Production

Chasanah¹,

Pratitis²,

Mangunwardoyo³

2013

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Production of marine bioactive compound for commercial usage has been hampered due tothe problem of raw material supply. To overcome this, marine microbes especially those associatedwith the bioactive-compound producer biota, has been explored as bioactive sources, with severaladvantages such as shorter production time, cheaper production cost and avoiding over exploitationof marine biota sources. Previous research showed that fungi MFW 23-08 was one of the potentialisolates from Wakatobi sponges which produced bioactive compounds that was active againstbreast cancer cell line and as antioxidant. This study was intended to identify MFW 23-08 andoptimize the production of its bioactive compound through optimization of MFW 23-08 culture.Culture optimization was conducted using 3 liquid media, i.e. malt extract broth (MEB), glucosepeptone yeast (GPY), and minimal fungal media (MFM), and cultivation periods, i.e. 2, 4, 6, 8, and10 wk. Results revealed that MFW 23-08 crude extract of 2 wk-MFM cultivation, at the concentrationof 30 μg/ml, was able to retard 87% breast cancer (T47D) cell growth. While at concentration of100 μg/ml, the 6 wk. MEB cultivated extract was able to hamper free radicals (56%). However, thecrude extract from MFM media cultivation, in the concentration of 50 and 100 μg/ml was not able toinhibit Escherichia coli and Staphylococcus aureus growth. Based on molecular identificationusing ITS1-ITS4 primers, MFW 23-08 isolate was 99% similar to Penicillium citrinum, P.griseofolvum and Penicillium sp.

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Section: Resultsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 86%

Identification and Cultivation of MFW 23-08 Isolated From Marine Sponges for Bioactive Compound Production

Chasanah¹,

Pratitis²,

Mangunwardoyo³

2013

Squalen Bull. Marine Fisheries Postharvest Biotech.

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“…Kapang hasil kultivasi disaring dengan kertas saring untuk memisahkan antara filtrat dan miselium. Ekstraksi metabolit sekunder kapang mengacu pada metode Nursid et al (2010). Proses ekstraksi dibagi menjadi 2 tahap, yaitu ekstraksi miselium kapang dan ekstraksi filtrat hasil kultivasi.…”

Section:  Ekstraksi Metabolit Sekunder Kapang Tanahunclassified

Eksplorasi Antibakteri dari Kapang Tanah Arboretum

Rochman

Sunartatie

Afiff

2020

J. Ilmu. Pertan. Indones.

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Research to discover new antibacterial agent has been increasing due to the increasing number of pathogenic bacteria become antibiotic-resistant. Microbiomes in soil mold have antibacterial potency by producing secondary metabolites. The aim of this research was to isolate soil molds from the Faculty of Forestry Arboretum at IPB University. Arboretum’s soil was sampled ± 7.5 g from several points with maximum depth of 15 cm from surface. Mold isolate were cultivated for 28 days using waterbath shaker. Cultivated mycelium and soil filtrate were then separated and extracted using different methods and solvents, prior to antibacterial activity test using agar well diffusion method. Six isolates from Aspergillus, Penicillium, Paecilomyces, and Verticilium were used in this research. Cultivatation using sabouraud dextrose broth (SDB) contains pepton and dextrose in 25°C capable of stimulating mold growth. The research showed the result of secondary metabolit had antibacterial characteristic toward tested bacteria. The results of antibacterial activity test on both mycelium and soil filtrate extract showed that soil mold isolates had antibacterial activity against Staphylococcus aureus and Escherichia coli. Isolate 103A originated from soil filtrate formed the largest inhibition zone on both tested pathogens. Keywords: antibacteria, mycelium, soil filtrate, soil mold

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“…Fungi extraction was conducted according to Nursid, Pratitis, and Chasanah (2010). Mycelium and broth were extracted separately.…”

Section: Fungi Extractionmentioning

confidence: 99%

Screening of Marine-Derived Fungi Extracts as Antioxidant, Tyrosinase Inhibitor, and Antiglycation

Nursid

Marraskuranto

Septorini

et al. 2019

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Marine-derived fungi are of great interest as new promising sources of bioactive secondary metabolites. The aim of this study was to determine the antioxidant activity, tyrosinase inhibitor and antiglycation of marine-derrived fungi collected from Kepulauan Seribu Marine National Park, Indonesia. Antioxidant screening was determined using the 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. The tyrosinase inhibitor was screened using L-tyrosine substrate, while the antiglycation test was determined by the ability to inhibit the formation of advanced glycation end products (AGEs). A total of 28 marine fungi isolates had been screened for their activities. Mycelium extract of MFP 271 had the best antioxidant activity with the IC50 of 287.25 ± 50 µg/mL. Mycelium extract of MFP 277 had the best tyrosinase inhibitory activity with the IC50 of 586.42 µg/mL. The MFP 274-broth extract had the highest antiglycation activity with the IC50 value of 298.57 µg/mL. Based on the screening, the crude extracts were considered weak as antioxidant, but tyrosinase inhibitor and antiglycation activity of MFP 277 and MFP 274 are needed to investigate in depth activities.

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Kultivasi Fungi Mfw-01-08 yang Diisolasi dari Ascidia Aplidium longithorax dan Uji Aktivitas Sitotoksiknya Terhadap Sel Kanker Payudara T47D

Cited by 8 publications

References 0 publications

Identification and Cultivation of MFW 23-08 Isolated From Marine Sponges for Bioactive Compound Production

Identification and Cultivation of MFW 23-08 Isolated From Marine Sponges for Bioactive Compound Production

Eksplorasi Antibakteri dari Kapang Tanah Arboretum

Screening of Marine-Derived Fungi Extracts as Antioxidant, Tyrosinase Inhibitor, and Antiglycation

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