Kurstakins:  A New Class of Lipopeptides Isolated from Bacillus thuringiensis

Hathout, Yetrib; Ho, Yen‐Peng; Ryzhov, Victor; Demirev, Plamen A.; Fenselau, Catherine

doi:10.1021/np000169q

Cited by 156 publications

(129 citation statements)

References 29 publications

Supporting

Mentioning

121

Contrasting

Unclassified

Order By: Relevance

“…14,17,18 Previously, the identification of Bacillus spp. using MALDI-TOF MS was mainly based on a comparison of mass spectra of proteins, [19][20][21][22][23][24][25][26] lipopeptides, 27-29 and peptides. 30,31 The MALDI-TOF MS method relies on reproducible detection of microbial protein patterns, thus providing complementary information to genotypic or phenotypic test methods.…”

Section: Introductionmentioning

confidence: 99%

Lipid fingerprinting of Bacillus spp. using online MALDI-TOF mass spectrometry

et al. 2012

View full text Add to dashboard Cite

Rapid identification of Bacillus spores has attracted great interest in the medical and forensic communities due to the highly pathogenic nature of certain Bacillus species and its potential application in bioweapons. In this study, we evaluated the feasibility of rapid and reliable identification of several Bacillus spp. (B. subtilis, B. cereus, B. megaterium, B. pumilus, and B. sphaericus) using online MALDI-TOF mass spectrometry combined with lipid profiling. The bacterial cultures in liquid medium were extracted with methanol and chloroform, and the extracts were aerosolized into droplets and immediately analyzed with an online MALDI-TOF mass spectrometer. Results showed that phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and diglycosyldiacylglycerol (DGDG) were present in the mass spectra, as alkali metal adducts. The unique lipid profiles obtained from these Bacillus spp. allowed us to differentiate between them. The reliability and reproducibility of the rapid identification method for Bacillus were tested under different experimental conditions. An alternative online chemotaxonomic method for bacterial identification is demonstrated.

show abstract

Section: Introductionmentioning

confidence: 99%

Lipid fingerprinting of Bacillus spp. using online MALDI-TOF mass spectrometry

et al. 2012

View full text Add to dashboard Cite

show abstract

“…B. megaterium, B. coagulans, B. thuringiensis BT2 and several B. amyloliquefaciens isolates demonstrated both strong biosurfactant activity and suppression of B. cinerea, F. pallidoroseum, and F. moniliforme, implying the ability to synthesize antifungal lipopeptides. The production of antifungal lipopeptides has been identified in other strains of the species of Bacillus used in this study, including B. amyloliquefaciens [11,12,24], B. coagulans [21], B. megaterium [23], and B. thuringiensis [15,16,57]. B. pumilus and O. sojae demonstrated weak biosurfactant activity but no fungal suppression.…”

Section: Bacillus Species Oceanobacillus Sojae and Ochrobactrum Antmentioning

confidence: 89%

Antifungal Screening of Bioprotective Isolates against Botrytis cinerea, Fusarium pallidoroseum and Fusarium moniliforme

Senna

Lathrop

2017

Fermentation

View full text Add to dashboard Cite

Abstract:The fungi Botrytis cinerea, Fusarium pallidoroseum, and Fusarium moniliforme are the causative agents of several plant diseases and can cause significant crop loss both before and after harvest. Fungicides are employed to control these phytopathogens, but fungicide use has led to an increase in resistance and may negatively affect the environment and human health. Hence, more environmentally sustainable solutions such as biological control methods are needed. The purpose of this study was to screen 22 bacterial isolates for inhibitory activity against fungal phytopathogens. To evaluate antifungal activity, the bacterial isolates were individually spot-inoculated onto Tryptic Soy Agar or de Man, Rogosa, Sharpe agar, and then a plug of fungal-colonized agar was placed onto the center of the isolate-inoculated plate. Plates were incubated at 24 • C for 10 days and fungal growth was evaluated. Nine of the 22 isolates screened inhibited all three fungi; inhibition by these isolates ranged from 51-62%, 60-68%, and 40-61% for B. cinerea, F. pallidoroseum, and F. moniliforme, respectively. Isolates were also screened for biosurfactant activity using the drop-collapse test. Bacillus megaterium, Bacillus coagulans, Bacillus thuringiensis and three Bacillus amyloliquefaciens isolates demonstrated strong biosurfactant activity and suppression of all three fungi, and therefore are recommended for further study.

show abstract

“…The potential of Bacillus sp. to synthesise a wide variety of metabolites with antifungal activity is known and in recent years it has been a subject of experiments [41][42][43]. Most of these substances belong to lipopeptides, especially from surfactin, iturin and fengicin classes.…”

Section: Discussionmentioning

confidence: 99%

Soil Drench with Fungicides Alternatives Against Root Rot Incidence of Some Vegetables Under Greenhouse Conditions

Abdel-Kader¹,

El-Mougy²,

Aly³

et al. 2012

IJAF

View full text Add to dashboard Cite

Soil drench with furfural, Humic & Folic acid and/or bio-agents treatments were evaluated against root rot incidence of Cucumber, Cantaloupe, Tomato and Pepper in pot experiments. The pathogenic fungi, Fusarium solani, F. oxysporum, Rhizoctonia solani, Sclerotium rolfsii, Sclerotinia sclerotiorum, S. minor, Macrophomina phaseolina, Alternaria solani and Pythium sp. As well as bio-agents, T. harzianum, T. viride, Bacillus subtilis and Pseudomonas fluorescens were used in this study. The obtained results revealed the efficacy of applied some of plant resistance inducers and/or bio-agents as soil drench against tested vegetables root diseases incidence at both pre-, and post-emergence growth stages comparing with untreated control. Applied treatments of the bio-agents in combination with the tested chemicals resulted in higher significant reduction in root rot incidence than each of them alone. Treatments of T. harzianum either alone or combined with chemicals were superior for reducing root rot disease for all tested vegetable plants followed by B. subtilis treatments comparing with the other tested ones. More detailed studies are required to elucidate formulations of antagonists and alternative fungicides for more successful protection against such soil-borne diseases.

show abstract

Kurstakins: A New Class of Lipopeptides Isolated from Bacillus thuringiensis

Cited by 156 publications

References 29 publications

Lipid fingerprinting of Bacillus spp. using online MALDI-TOF mass spectrometry

Lipid fingerprinting of Bacillus spp. using online MALDI-TOF mass spectrometry

Antifungal Screening of Bioprotective Isolates against Botrytis cinerea, Fusarium pallidoroseum and Fusarium moniliforme

Soil Drench with Fungicides Alternatives Against Root Rot Incidence of Some Vegetables Under Greenhouse Conditions

Contact Info

Product

Resources

About