Kyasanur Forest Disease in India: innovative options for intervention

Paramasivan, R

doi:10.1080/21645515.2019.1602431

Cited by 20 publications

(10 citation statements)

References 14 publications

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“…Another challenge is genetic diversity of virus that resulted in immune escape, always-recombinant vaccines design prefers to target conserved antigenic regions of virus, it might accelerates better immune response 23 . The KFDV vaccinated individuals were reported for disease occurrence, it may because of variations in circulating virus 24 . Therefore, we picked the conserved region of KFDV envelope protein sequence, to overcome the genetic diversity of viral strains.…”

Section: Discussionmentioning

confidence: 99%

In-silico design of envelope based multi-epitope vaccine candidate against Kyasanur forest disease virus

Arumugam

Prasad

2021

Sci Rep

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Kyasanur forest disease virus (KFDV) causing tick-borne hemorrhagic fever which was earlier endemic to western Ghats, southern India, it is now encroaching into new geographic regions, but there is no approved medicine or effective vaccine against this deadly disease. In this study, we did in-silico design of multi-epitope subunit vaccine for KFDV. B-cell and T-cell epitopes were predicted from conserved regions of KFDV envelope protein and two vaccine candidates (VC1 and VC2) were constructed, those were found to be non-allergic and possess good antigenic properties, also gives cross-protection against Alkhurma hemorrhagic fever virus. The 3D structures of vaccine candidates were built and validated. Docking analysis of vaccine candidates with toll-like receptor-2 (TLR-2) by Cluspro and PatchDock revealed strong affinity between VC1 and TLR2. Ligplot tool was identified the intermolecular hydrogen bonds between vaccine candidates and TLR-2, iMOD server confirmed the stability of the docking complexes. JCAT sever ensured cloning efficiency of both vaccine constructs and in-silico cloning into pET30a (+) vector by SnapGene showed successful translation of epitope region. IMMSIM server was identified increased immunological responses. Finally, multi-epitope vaccine candidates were designed and validated their efficiency, it may pave the way for up-coming vaccine and diagnostic kit development.

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Section: Discussionmentioning

confidence: 99%

In-silico design of envelope based multi-epitope vaccine candidate against Kyasanur forest disease virus

Arumugam

Prasad

2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Another challenge is genetic diversity of virus that resulted in immune escape, alwaysrecombinant vaccines design prefers to target conserved antigenic regions of virus, it might accelerates better immune response 20 . The KFDV vaccinated individuals were reported for disease occurrence, it may because of variations in circulating virus 21 . Therefore, our study has picked the conserved region of KFDV envelope protein sequence, to overcome the genetic diversity of viral strains.…”

Section: Discussionmentioning

confidence: 99%

In-Silico Design of Envelope based Multi-epitope Vaccine Candidate Against Kyasanur Forest Disease Virus

Arumugam

2021

Preprint

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Kyasanur Forest Disease Virus (KFDV) causing common tick-borne hemorrhagic fever in south India, there is no approved anti-viral or efficacious vaccine against this disease. Recent KFDV spread into new geographic locations gives urgent call for development of new vaccine and drugs. In this study, we adapted in-silico approach to design multi-epitope subunit vaccine for KFDV. Conserved regions of KFDV envelope protein sequences reported during 1962 to 2016 were identified. Eight different immuno-informatics tools were employed to predict the linear B-cell and T-cell epitopes, high scored and/or multi-immunogenic epitopes were linked together and obtained two vaccine candidates (VC1 and VC2). Obtained vaccine candidates were found to be non-allergic and had good antigenic properties, also gives the cross-protection against to Alkhurma Hemorrhagic Fever virus (AHFV). The 3D structures of vaccine candidates were built and validated. Docking of vaccine candidates with toll-like receptor-8 (TLR-8) was performed by Hex 8.0 and Cluspro, highest binding energy observed between VC2 and TLR8. JCAT sever confirmed cloning efficiency of both vaccine constructs and in-silico cloning into pET30a (+) vector by SnapGene suggests successful translation of vaccine constructs. In this study, multi-epitope vaccine candidates were designed and validated, it paves the way for up-coming vaccine and diagnostic kit development.

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“…Vaccination against Hendra virus was practiced in Australia and is also reported effective against Nipah virus (Broder et al, 2013). In India, prophylactic vaccines are available against JE (Kulkarni et al, 2018), H1N1 influenza (Murhekar and Mehendale, 2016) and KFD (Rajaiah, 2019) for use in endemic areas of the country. WHO has recently qualified Ebola virus vaccines (Vesiculo virus vector containing G glycoprotein of Ebola virus) for its use in african countries (Nature, 2019).…”

Section: Vaccination and Health Managementmentioning

confidence: 99%