l-arginine minimizes immunosuppression and prothrombin time and enhances the genotoxicity of 5-fluorouracil in rats

Fagiani, Marcela de Andrade Bernal; Fluminhan, Antônio; Mello, Fabíola de Azevedo; Yabuki, Denise; Gonçalves, Gracielle Vieira; Tsujigushi, Leticia Kazama; Pereira, Liliane Girotto; Silva, Kesia Araújo da; Silva, Sara Bertozo Bezerra da; Laposy, Cecília Braga; Giozo, Paulo Felipe Izique; Reis, Luis Souza Lima de Souza

doi:10.1016/j.nut.2019.04.012

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…may be attributed to the improved TAC in these groups causing diminished O 2 − and ONOO production. Both LA and LC had been documented to regulate immune function and inflammatory response [68,69]. They play fundamental roles in restraining OS and destructive inflammatory reaction [69,70] hence, mitigate neural injury.…”

Section: Discussionmentioning

confidence: 99%

Neurotoxic Effect of Fipronil in Male Wistar Rats: Ameliorative Effect of L-Arginine and L-Carnitine

Mahmoud

Ali

Abdelrazek

et al. 2021

Biology

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The ameliorative effect of L-arginine (LA) and L-carnitine (LC) against fipronil (FPN)-induced neurotoxicity was explored. In this case, 36 adult male rats were randomly divided into six groups: group I received distilled water, group II received 500 mg/kg LA, group III received 100 mg/kg LC, group IV received 4.85 mg/kg FPN, group V received 4.85 mg/kg FPN and 500 mg/kg LA and group VI received 4.85 mg/kg FPN and 100 mg/kg LC for 6 weeks. Cognitive performance was assessed using Barnes maze (BM). Serum corticosterone, brain total antioxidant capacity (TAC), malondialdehyde (MDA) and dopamine were measured. Histopathology and immunohistochemistry of ionized calcium-binding adaptor (Iba-1), doublecortin (DCX) and serotonin (S-2A) receptors were performed. Fipronil induced noticeable deterioration in spatial learning and memory performance. In addition, FPN significantly (p < 0.05) diminished brain antioxidant defense system and dopamine coincide with elevated serum corticosterone level. Histopathological examination revealed degenerative and necrotic changes. Furthermore, Iba-1 and DCX were significantly expressed in cortex and hippocampus whereas S-2A receptors were significantly lowered in FPN group. However, administration of LA or LC alleviated FPN-induced deteriorations. In conclusion, LA and LC could be prospective candidates for mitigation of FPN-induced neurotoxicity via their antioxidant, anti-inflammatory and neuropotentiating effects.

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Section: Discussionmentioning

confidence: 99%

Neurotoxic Effect of Fipronil in Male Wistar Rats: Ameliorative Effect of L-Arginine and L-Carnitine

Mahmoud

Ali

Abdelrazek

et al. 2021

Biology

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“…Arginine, a semi-essential AA, plays a crucial role in the maintenance of immune function and exerts antiinflammatory functions (de Andrade Bernal Fagiani et al, 2019). Those characteristics help explain the benefit of Arg in helping reduce postoperative infection (Popovic et al, 2007).…”

Section: Methionine and Arginine Supplementation Alter Inflammatory A...mentioning

confidence: 99%

Methionine and arginine supplementation alter inflammatory and oxidative stress responses during lipopolysaccharide challenge in bovine mammary epithelial cells in vitro

Dai

Coleman

et al. 2020

Journal of Dairy Science

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Mastitis, inflammation of the udder, is one of the most common diseases hampering milk yield of dairy cows. Methionine (Met) and arginine (Arg) are key nutrients with potential to regulate inflammation and oxidative stress. The aim of this study was to evaluate the effect of increased supply of Met and Arg on mRNA and protein abundance associated with innate immune response and redox balance during lipopolysaccharide (LPS) stimulation in primary bovine mammary epithelial cells (BMEC). Primary BMEC (n = 4 replicates per treatment) were pre-incubated for 12 h in media with the following amino acid combinations: ideal profile of amino acids (control; Con), increased Met supply (incMet), increased Arg supply (incArg), and increased supply of Met and Arg (incMetArg). Subsequently, cells were challenged with or without LPS (1 µg/mL) and incubated for 6 h. Data were analyzed as a 2 × 2 × 2 factorial using the MIXED procedure of SAS 9.4 (SAS Institute Inc., Cary, NC). The downregulation of SLC36A1 and SLC7A1 mRNA abundance induced by LPS was attenuated in the incArg cultures. Although challenge with LPS led to lower abundance of proteins related to the antioxidant response (NFE2L2, NQO1, GPX1), lower levels of ATG7, and lower mRNA abundance of GPX3, we found little effect in cultures with incMet or incArg. Cultures with incMet, incArg, or incMetArg led to attenuation of the upregulation of SOD2 and NOS2 induced by LPS. Abundance of phosphorylated p65 (RELA) was greater after LPS stimulation, but the response was attenuated in cultures with incMet. The greater ratio of pRELA to total RELA in responses to LPS was also attenuated in cultures with incMetArg. The greater mRNA abundance of the proinflammatory cytokine IL1B induced by LPS was attenuated in cultures with incMet, and the same trend induced by LPS on CXCL2 was also alleviated in cultures with incArg. Overall, the data suggest that greater supply of Met and Arg alleviated the proinflammatory responses triggered by LPS through controlling the abundance of proinflammatory cytokines and chemokines and activity of NF-κB. Little benefit on oxidative stress induced by LPS challenge in BMEC was detected with greater supply of Met and Arg.

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“…Glutathione peroxidases (GPx) activity relies on GSH availability and plays a fundamental role in cellular protection against reactive oxygen metabolites (ROM; Liang et al, 2020). In addition, the semi-essential AA arginine (Arg) is also important for immune capacity and has antiinflammatory properties (de Andrade Bernal Fagiani et al, 2019). Previous studies underscored the role of Arg as a functional AA with benefits on the modulation of inflammatory responses in primary bovine mammary epithelial cells (BMEC; Wu et al, 2016) and lactating dairy cows (Zhao et al, 2018) and also through an increase in GSH levels (Dasgupta et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Alterations in immune and antioxidant gene networks by gamma-d-glutamyl-meso-diaminopimelic acid in bovine mammary epithelial cells are attenuated by in vitro supply of methionine and arginine

Dai

Coleman

Lopes

et al. 2021

Journal of Dairy Science

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l-arginine minimizes immunosuppression and prothrombin time and enhances the genotoxicity of 5-fluorouracil in rats

Cited by 7 publications

References 22 publications

Neurotoxic Effect of Fipronil in Male Wistar Rats: Ameliorative Effect of L-Arginine and L-Carnitine

Neurotoxic Effect of Fipronil in Male Wistar Rats: Ameliorative Effect of L-Arginine and L-Carnitine

Methionine and arginine supplementation alter inflammatory and oxidative stress responses during lipopolysaccharide challenge in bovine mammary epithelial cells in vitro

Alterations in immune and antioxidant gene networks by gamma-d-glutamyl-meso-diaminopimelic acid in bovine mammary epithelial cells are attenuated by in vitro supply of methionine and arginine

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