Natural attenuation is described as a naturally occurring process, mostly in soils and also in groundwater, without human intervention, which transforms, reduces and destroys the organic and inorganic contaminants. As an eco-friendly, cost-effective and relatively simple technology, natural attenuation is widely used for the treatment of contaminated soils. However, the application of this technology must be carefully controlled and monitored not only for its efficiency and durability over time, but also for the migration of contaminants to ensure no risk to human health and ecosystems. Furthermore, the success of this technique requires a good knowledge of the type of contaminants, the physical and chemical characteristics of the soils, as well as the living actors, including plants, fauna, microorganisms and their interactions, that live in the soils to be treated and that will be involved in this process. The purpose of this chapter is to provide the most recent information regarding the principle of this technology, the role of the living actors and the interactions between plant, fauna and microorganisms, the advantages and disadvantages, and finally to discuss the efficiency of this technique in comparison with other techniques such as phytoremediation or bioremediation. In fine, we will discuss its social acceptability.