L929 Cell Conditioned Medium Protects RAW264.7 Cells From Oxidative Injury Through Inducing Antioxidant Enzymes

Pang, Zhan-Jun; Yuan, Chen

doi:10.1006/cyto.2000.0683

Cited by 12 publications

(3 citation statements)

References 22 publications

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“…The present results were opposite to our previous findings indicating that M-CSF reduced oxidative injury to RAW264.7 cells under certain conditions [24,25]. Several differences between the two experiments may explain the difference.…”

Section: The Morphological Change Of Raw2647 Cells Treated With Tboohcontrasting

confidence: 99%

“…Treatment of WHHL rabbits with rhM-CSF increased M-CSF staining in foam cells, which correlated with a reduced cholesterol content of these cells. In our previous research, exogenous M-CSF could alleviate tbOOH-induced oxidative injury to monocytes/macrophages through inducing the antioxidant system [24,25]. The antioxidant system included reduced glutathione (GSH) and antioxidant enzymes such as glutathione peroxidase (GPx) and manganese superoxide dismutase (MnSOD).…”

Section: The Morphological Change Of Raw2647 Cells Treated With Tboohmentioning

confidence: 99%

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Effect of macrophage colony stimulating factor overexpression on oxidative injury/resistance of RAW264.7 cells

2003

Clinical and Experimental Medicine

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Oxidative injury to monocytes/macrophages is considered one of the key factors in atherogenesis. Macrophage colony stimulating factor (M-CSF) also plays an important role in the stages of atherosclerosis. Some researchers showed that M-CSF accelerated pathological changes in the early stages of atherosclerosis. However, other reports suggested that exogenous M-CSF could prevent the progress of atherosclerosis. To further investigate the role of M-CSF in atherogenesis and to elucidate the effect of M-CSF on the oxidative injury to monocytes/macrophages, RAW264.7 cell lines overexpressing M-CSF were established by applying the lipofectin transfection method. The oxidative injurious effect of tert-butylhydroperoxide on the established cell lines was investigated. Two M-CSF-transfected RAW264.7 cell lines secreted large amounts of M-CSF. Compared with the non-transfected RAW264.7 cells, M-CSF-overexpressing RAW264.7 cells were more vulnerable to oxidative injury. We conclude that M-CSF could aggravate the oxidative injury due to macrophages in some situations.

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Section: The Morphological Change Of Raw2647 Cells Treated With Tboohcontrasting

confidence: 99%

Section: The Morphological Change Of Raw2647 Cells Treated With Tboohmentioning

confidence: 99%

Effect of macrophage colony stimulating factor overexpression on oxidative injury/resistance of RAW264.7 cells

2003

Clinical and Experimental Medicine

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“…An oxidant, tbOOH was used for free radical source when the oxidative injury to cells was investigated [19,20]. HDFs (1 Â 10 3 cells/well) were plated in 96-well plates in DMEM/F12 with 0.1% FBS for 24 h to arrest mitosis.…”

Section: Cell Survival Assaymentioning

confidence: 99%

Evidence supporting antioxidant action of adipose-derived stem cells: Protection of human dermal fibroblasts from oxidative stress

Kim

Park²,

Kim³

et al. 2008

Journal of Dermatological Science

241

148

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Expression of transforming growth factor-β and insulin-like growth factor in molar and placental tissues

Pang

Xing

2002

Arch Gynecol Obstet

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The semiquantitative reverse transcription polymerase chain reaction was employed to detect the expression of transforming growth factor beta (TGF-beta) and insulin-like growth factor (IGF) in complete hydatidiform mole, normal first-trimester villi, the normal term placenta (after vaginal/abdominal deliver) and the preeclamptic placenta at term. The expression of IGF-I mRNA was seen in all five tissues, but its level was much lower in the term placental tissues with preeclampsia than in other tissues. The content of IGF-I mRNA in villous tissues from molar pregnancy was slightly higher than in normal first-trimester villi. IGF-II mRNA was detected at similar levels in all three sorts of term placental tissues. However, the expression level of IGF-II mRNA in tissues of complete molar pregnancy was significantly lower than in normal first-trimester villi. TGF-beta(3) was found expressed in all five tissues, while TGF-beta(1) and TGF-beta(2) mRNA were not detected. Compared to the normal first-trimester villi, the expression of TGF-beta(3) in complete hydatidiform molar tissues was comparatively higher. Furthermore, the expression levels of TGF-beta(3) in the preeclamptic placenta and the normal placenta after cesarean birth were higher than in the placenta after vaginal delivery. We concluded that, the change of TGF-beta and IGF expression in placental tissues might be involved in the development of trophoblastic diseases of pregnancy.

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L929 Cell Conditioned Medium Protects RAW264.7 Cells From Oxidative Injury Through Inducing Antioxidant Enzymes

Cited by 12 publications

References 22 publications

Effect of macrophage colony stimulating factor overexpression on oxidative injury/resistance of RAW264.7 cells

Effect of macrophage colony stimulating factor overexpression on oxidative injury/resistance of RAW264.7 cells

Evidence supporting antioxidant action of adipose-derived stem cells: Protection of human dermal fibroblasts from oxidative stress

Expression of transforming growth factor-β and insulin-like growth factor in molar and placental tissues

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