Label-Free and Recalibrated Multilayer MoS₂ Biosensor for Point-of-Care Diagnostics

Abstract

Molybdenum disulfide (MoS) field-effect transistor (FET)-based biosensors have attracted significant attention as promising candidates for highly sensitive, label-free biomolecule detection devices. In this paper, toward practical applications of biosensors, we demonstrate reliable and quantitative detection of a prostate cancer biomarker using the MoS FET biosensor in a nonaqueous environment by reducing nonspecific molecular binding events and realizing uniform chemisorption of anti-PSA onto the MoS surface. A systematic and statistical study on the capability of the proposed device is presented, and the biological binding events are directly confirmed and characterized through intensive structural and electrical analysis. Our proposed biosensor can reliably detect various PSA concentrations with a limit of 100 fg/mL. Moreover, rigorous theoretical simulations provide a comprehensive understanding of the operating mechanism of the MoS FET biosensors, and further suggests the enhancement of the sensitivity through engineering device design parameters.

Membraneless reproducible MoS2 field-effect transistor biosensor for high sensitive and selective detection of FGF21

“…For MoS 2 FET biosensor, functionalization of MoS 2 is also an important process for the interactions between these 2D materials and biomolecules. In a nonaqueous environment of our experiment, there is less interference of Debye length [27]. The consistent current change result indicates that the MoS 2 FET devices we prepared have satisfactory reproducibility.…”

“…This process can provide experimental evidence for the consecutive trends of current change caused by the FGF21 concentration gradients. The 'pH memory theory' can be utilized as an excellent theoretical basis for achieving highly accurate and reproducible biosensor operation [27].…”

“…Recent reports have demonstrated MoS 2 FETs were used for electrical detection of various biomolecules. Park et al [27] investigated the FET device using multi-layered MoS 2 with alumina (Al 2 O 3 ) as a protective layer for detecting prostate specific antigen (PSA) to achieve high sensitivity, with a limit of 100 fg mL −1 . Lee et al [28] In addition, the non-aqueous environment offering excellent reliability and repeatability was used to quickly and accurately detect FGF21, reducing the influence of leakage.…”

Large area few-layer TMD film growths and their applications

“…Inherent n-typing and p-typing, given the material, enables digital logic circuitry in limited cases. Their thinness means they are very exposed and thus responsive to their environment, priming them for sensing applications and data storage/retrieval [24,[72][73][74].…”

High-Sensitivity Detection of the Lung Cancer Biomarker CYFRA21-1 in Serum Samples Using a Carboxyl-MoS2 Functional Film for SPR-Based Immunosensors

“…MoS 2 is composed of molybdenum (Mo) and sulfur (S) in sandwich layers, and it is one of the most studied two-dimensional layered transition metal dichalcogenide (TMDC) nanomaterials. Park et al used multilayer MoS 2 -based FET biosensors to detect prostate cancer antigen (PSA) at concentrations as low as 100 fg/mL (Park et al, 2017). In addition, Yoon et al (2017) used a MoS 2 nanoparticle-GO hybrid structure in an electrochemical biosensor composed of myoglobin to detect H 2 O 2 with an LOD of 20 nM.…”