Label-free, chemiresistor immunosensor for stress biomarker cortisol in saliva

Tlili, Chaker; Myung, Nosang V.; Shetty, Vivek; Mulchandani, Ashok

doi:10.1016/j.bios.2011.04.045

Cited by 104 publications

(72 citation statements)

References 17 publications

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“…26,27 The sensitivity of the biosensor is a critical parameter since the biomarkers in saliva are usually present at very low concentration. 28 Compared to other FET biosensors, the diameter of a silicon nanowire (SiNW) is comparable to the dimensions of many biological relevant molecules, offering an opportunity for efficient bioaffinity and high sensitivity in detecting biomarkers. 29 Again, thanks to the SiNW very small dimension, the SiNW obtains a high surface-to-volume ratio, allowing the SiNW FET biosensor to be a very sensitive tool for biomolecule detection.…”

Section: Introductionmentioning

confidence: 99%

Silicon Nanowire Biosensor for Highly Sensitive and Multiplexed Detection of Oral Squamous Cell Carcinoma Biomarkers in Saliva

Zhang

Rong-mei

et al. 2015

ANAL. SCI.

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Silicon nanowire (SiNW) field-effect transistor (FET) biosensors have already been used as powerful sensors for the direct detection of disease-related biomarkers. However, the multiplexed detection of biomarkers in real samples is still challenging. Interleukin 8 (IL-8) and tumor necrosis factor α (TNF-α) are two typical biomarkers of oral squamous cell carcinoma (OSCC). In this study, we developed a multiplexed detection methodology for IL-8 and TNF-α detection in saliva using SiNW FET biosensors. We fabricated the SiNW FET sensors using a top-down lithography fabrication technique. Subsequently, we achieved the multiplexed detection of two biomarkers in saliva by specific recognition of the two biomarkers with their corresponding antibodies, which were modified on the SiNW. The established method was found to have a limit of detection as low as 10 fg/mL in 1×PBS as well as 100 fg/mL in artificial saliva. Because of its advantages, including label-free and multiplexed detection, non-invasive analysis, highly sensitive and specific determination, the proposed method is expected to be widely used for the early diagnosis of OSCC.

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Section: Introductionmentioning

confidence: 99%

Silicon Nanowire Biosensor for Highly Sensitive and Multiplexed Detection of Oral Squamous Cell Carcinoma Biomarkers in Saliva

Zhang

Rong-mei

et al. 2015

ANAL. SCI.

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“…Thanks to their high conductivity and large surface-to-volume ratio, small changes on the conformation or displacement of the immobilized bioreceptors can be detected (243,244). This transduction mechanism has been reported in field-effect transistors (FET) (2,69,245,246) and may provide the basis of future devices like sensor-based bioelectronic noses and tongues (114,236,247), although, at present, the response patterns obtained by these devices are not stable over time (2).…”

Section: Applications Of Carbon Nanotubes and Graphene In Biomedical mentioning

confidence: 99%

Properties and Customization of Sensor Materials for Biomedical Applications

Zuliani¹,

Curto²,

Matzeu³

et al. 2014

Comprehensive Materials Processing

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“…Their physical properties are strongly dependent on the way that graphitic sheets are wrapped to form the tubes (chirality), causing them to exhibit metallic or semiconducting characteristics [99]. In particular, SWCNTs have been explored as promising building blocks in the construction of FET-based immunosensors with improved sensibility [58,69,76,[80][81][82]103]. SWCNTs display high electron transfer because all carbon atoms are present in their surface.…”

Section: Carbon Nanotubesmentioning

confidence: 99%

“…Therefore, the displacement represents a successful strategy for detecting such target analytes. For example, Tliti et al described a label-free FET immunosensor based on SWCNT for the ultrasensitive detection of stress biomarker cortisol in saliva [82]. SWCNTs were covalently functionalized with a cortisol analog (cortisol-3-CMO-NHS ester) followed by covalent conjugation with monoclonal anti-cortisol antibodies (large size and charged proteins).…”

Section: Carbon Nanotubesmentioning

confidence: 99%

“…SWCNTs were covalently functionalized with a cortisol analog (cortisol-3-CMO-NHS ester) followed by covalent conjugation with monoclonal anti-cortisol antibodies (large size and charged proteins). This chemiresistive biosensor was able to detect small size and uncharged cortisol molecules because the antibody bound to the SWCNT functionalized with cortisol analog was stripped/displaced, provoking a large change of the device resistance/conductance and a LOD of 1 pg·mL −1 [82]. Tan et al also fabricated a label-free immunoFET based on SWCNT by the displacement assay of the immobilized antibodies [91].…”

Section: Carbon Nanotubesmentioning

confidence: 99%

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Recent Trends in Field-Effect Transistors-Based Immunosensors

Moraes

Kubota

2016

Chemosensors

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Abstract:Immunosensors are analytical platforms that detect specific antigen-antibody interactions and play an important role in a wide range of applications in biomedical clinical diagnosis, food safety, and monitoring contaminants in the environment. Field-effect transistors (FET) immunosensors have been developed as promising alternatives to conventional immunoassays, which require complicated processes and long-time data acquisition. The electrical signal of FET-based immunosensors is generated as a result of the antigen-antibody conjugation. FET biosensors present real-time and rapid response, require small sample volume, and exhibit higher sensitivity and selectivity. This review brings an overview on the recent literature of FET-based immunosensors, highlighting a diversity of nanomaterials modified with specific receptors as immunosensing platforms for the ultrasensitive detection of various biomolecules.

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Label-free, chemiresistor immunosensor for stress biomarker cortisol in saliva

Cited by 104 publications

References 17 publications

Silicon Nanowire Biosensor for Highly Sensitive and Multiplexed Detection of Oral Squamous Cell Carcinoma Biomarkers in Saliva

Silicon Nanowire Biosensor for Highly Sensitive and Multiplexed Detection of Oral Squamous Cell Carcinoma Biomarkers in Saliva

Properties and Customization of Sensor Materials for Biomedical Applications

Recent Trends in Field-Effect Transistors-Based Immunosensors

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